Rapamycin

Tocris Bioscience | Catalog # 1292

mTOR inhibitor; immunosuppressant
Tocris Bioscience

Key Product Details

Description

mTOR inhibitor; immunosuppressant
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Product Description

Rapamycin is an antifungal and immunosuppressant. Rapamycin is a specific inhibitor of mTOR (mammalian target of rapamycin); complexes with FKBP-12 and binds mTOR inhibiting its activity. Rapamycin inhibits interleukin-2-induced phosphorylation and activation of p70 S6 kinase and induces autophagy in yeast and mammalian cell lines. Rapamycin drives hPSC differentiation to mesendoderm and blood progenitor cells. Also used as a chemical dimerizer; rapamycin and GA3-AM chemically inducible dimerization systems are orthogonal. Inhibits MERS-CoV infection of Huh7 cells in a plaque reduction assay. Enhances lentiviral transduction of hematopoietic stem cells.

External Portal Information

Chemicalprobes.org

is a portal that offers independent guidance on the selection and/or application of small molecules for research. The use of Rapamycin is reviewed on the chemical probes website.

Product Specifications for Rapamycin

Molecular Weight

914.18

Formula

C51H79NO13

Storage

Store at -20°C

Purity

≥98% (HPLC)

Chemical Name

(3S,6R,7E,9R,10R,12R,14S,15E,17E,19E,21S,23S,26R,27R,34aS)-9,10,12,13,14,21,22,23,24,25,26,27,32,33,34,34a-Hexadecahydro-9,27-dihydroxy-3-[(1R)-2-[(1S,3R,4R)-4-hydroxy-3-methoxycyclohexyl]-1-methylethyl]-10,21-dimethoxy-6,8,12,14,20,26-hexamethyl-23,27-epoxy-3H-pyrido[2,1-c][1,4]oxaazacyclohentriacontine-1,5,11,28,29(4H,6H,31H)-pentone

CAS Number

53123-88-9

PubChem ID

5284616

InChI Key

QFJCIRLUMZQUOT-HPLJOQBZSA-N

SMILES

O=C([C@H](C)\C=C([C@@H](O)[C@@H](OC)C([C@@H]4C)=O)/C)C[C@]([C@H](C)C[C@H]2C[C@@H](OC)[C@H](O)CC2)([H])OC([C@@]1([H])N(C(C([C@@]3(O)[C@H](C)CC[C@](C[C@@H](/C(C)=C/C=C/C=C/[C@H](C4)C)OC)([H])O3)=O)=O)CCCC1)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 18.28 20

Preparing Stock Solutions for Rapamycin

The following data is based on the product molecular weight 914.18.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.2 mM 5.47 mL 27.35 mL 54.69 mL
1 mM 1.09 mL 5.47 mL 10.94 mL
2 mM 0.55 mL 2.73 mL 5.47 mL
10 mM 0.11 mL 0.55 mL 1.09 mL

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Product Documents for Rapamycin

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Rapamycin

For research use only

Related Research Areas

Autophagy

Customer Reviews for Rapamycin (3)

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  • Cytotoxicity testing
    Name: MARINA KOUTSIOUMPA
    Species: Human
    Assay Type: In Vitro
    Verified Customer | Posted 10/18/2022
    Studies were performed with rapamycin in chemotherapy-treated cultured cancer cell lines for the evaluation of cytotoxic effects. The product is highly recommended.
  • The role of mTOR signaling in genotoxics-induced stress.
    Name: MARINA KOUTSIOUMPA
    Species: Human
    Assay Type: In Vitro
    Cell Line/Tissue: MIA PaCa_2
    Verified Customer | Posted 06/10/2021
    Expected effects. IC50 equals 1nM upon 3 days of treatment of MIA PaCa_2 pancreatic cancer cell line.
  • rapamycin support human ES cells differentiation
    Name: Pinyuan Tian
    Species: Human
    Verified Customer | Posted 05/22/2019
    Rapamycin treatment (10nM) on human embryonic stem cells could enhance some cartilage differentiation markers such as SOX9.
    Rapamycin 1292

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