Mouse RAR beta/NR1B2 ELISA Kit (Colorimetric)

Novus Biologicals | Catalog # NBP3-00453

Novus Biologicals
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma, cell supernatant, and other biological fluids (100 ul)

Sensitivity

0.09 ng/mL

Assay Range

0.16 - 10 ng/mL
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Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Species

Mouse

Specificity

This kit recognizes Mouse RAR beta/NR1B2 in samples. No significant cross-reactivity or interference between Mouse RAR beta/NR1B2 and analogues was observed.

精度

Intra-Assay Precision (Precision within an assay) 4.62%

Inter-Assay Precision (Precision between assays) 4.96%

Recovery for Mouse RAR beta/NR1B2 ELISA Kit (Colorimetric)

Recovery

83 - 100%

Linearity

Mouse RAR beta/NR1B2 ELISA Kit (Colorimetric)

Scientific Data Images for Mouse RAR beta/NR1B2 ELISA Kit (Colorimetric)

Mouse RAR beta/NR1B2 ELISA Kit (Colorimetric)

ELISA: Mouse RAR beta/NR1B2 ELISA Kit (Colorimetric) [NBP3-00453] -

ELISA: Mouse RAR beta/NR1B2 ELISA Kit (Colorimetric) [NBP3-00453] - Standard Curve Reference

Kit Contents for Mouse RAR beta/NR1B2 ELISA Kit (Colorimetric)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: RAR beta/NR1B2

Retinoids can reverse various premalignant lesions as well as prevent some second primary tumors. The effects of retinoids are regulated by retinoic acid receptors (RAR) and retinoid X receptors, which act as ligand-activated transcription factors. Ligand-dependent transcriptional activation of RARs is a multistep process that ends with the formation of a multimeric co-activator complex on regulated promoters (1,2). Loss of RAR-beta expression and the accumulation of p53 and Ki67 proteins may help in the early identification of esophageal cancer in high-risk populations (3).

Long Name

Retinoic Acid Receptor, beta

Alternate Names

HAP, NR1B2, RARB, RRB2

Gene Symbol

RARB

Additional RAR beta/NR1B2 Products

Product Documents for Mouse RAR beta/NR1B2 ELISA Kit (Colorimetric)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Mouse RAR beta/NR1B2 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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FAQs for Mouse RAR beta/NR1B2 ELISA Kit (Colorimetric)

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    • Q: I am planning to perform IHC staining of RARB on FFPE samples. I have found the reference "J Cutan Pathol 2009;36; 1141-1145" used an antibody from your company. Would you let me know any other references using the antibody for IHC staining of RARB?
      A: Unfortunately we are not aware of any other publication wherein our Retinoic Acid Receptor beta antibodies were cited for their use in IHC application. From our sales records, it looks like the researcher used catalog number NB200-323 in the publication you mentioned. Retinoic Acid Receptor beta antibody NB200-323 is our best selling product among this category and it has also been cited for its use in WB application by Yang et al. in Am J Physiol Renal Physiol. 2008 Jun;294(6):F1433-40. We have at least 7 primary antibody products to Retinoic Acid Receptor beta that have been validated for IHC-P application.. We stand behind the quality of our products and offer 100% guarantee for the applications/species mentioned on the datasheets. We are always there to help if you come up with any difficulties and yes, we provide replacements/refunds after troubleshooting a problem, if you do not get your desired outcome! Therefore, please let us know if you have any specific questions or concerns on the IHC-P application of our Retinoic Acid Receptor beta antibodies. You may contact us at technical@novusbio.com.
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