Rat CD19 Antibody

Name: Rat CD19 Antibody
Brand: R&D Systems
SKU: AF7489
Price: 469 USD
Availability: InStock

R&D Systems | Catalog # AF7489

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Calculators
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Validated:

Rat

Cited:

Mouse

Applications

Validated:

Western Blot

Cited:

Immunohistochemistry

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG

View all CD19 Primary Antibodies »

Product Specifications

Immunogen

Chinese hamster ovary cell line recombinant rat CD19
Arg19-Gly287
Accession # NP_001013255

Specificity

Detects rat CD19 in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross-reactivity with recombinant human CD19 is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Rat CD19 Antibody

Detection of Rat CD19 by Western Blot.

Western blot shows lysates of rat splenocytes. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Rat CD19 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7489) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Specific bands were detected for CD19 at approximately 85-100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Rat CD19 Antibody

Application

Recommended Usage

Western Blot

1 µg/mL
Sample: Rat splenocytes

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: CD19

CD19 (also surface antigen B4 and Leu12) is a 95-110 kDa member of the Immunoglobulin superfamily of molecules. It is expressed by B cells, and interacts with CD21 for the purpose of reducing the threshold of the antigen signal needed to with activate the BCR. CD19 ligation also promotes B cell:follicular dendritic cell (FDC) interaction and B cell proliferation in the FDC zone of the spleen. Mature rat CD19 is a 529 amino acid (aa) type I transmembrane glycoprotein (aa 19-547). Based on mouse, it contains a 269 aa extracellular region (aa 19-287) plus a 236 aa cytoplasmic domain. The extracellular region possesses two C2-type Ig-like domains (aa 20-113 and 171-271) and one utilized phosphorylation site at Ser225. The cytoplasmic domain contains five potential Tyr phosphorylation sites. There is one splice form that shows a two aa substitution after Gly489. Over aa 19-287, rat CD19 shares 88% and 57% aa sequence identity with mouse and human CD19, respectively.