Rat CXCL2/GRO beta /MIP-2/CINC-3 Antibody

Catalog #: AF525
1 Citation Datasheet / COA / SDS

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AF525
AF525-SP

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Chemotaxis Induced by CXCL2/CINC‑3 and Neutralization by Rat CXCL2/CINC‑3 Antibody.

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All CXCL2/GRO beta/MIP-2/CINC-3 Antibodies

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Rat CXCL2/GRO beta /MIP-2/CINC-3 Antibody Summary

Species Reactivity

Rat

Specificity

Detects rat CXCL2/GRO beta /MIP‑2/CINC‑3 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 20% cross‑reactivity with recombinant rat (rr) CINC-2 alpha is observed, 15% cross-reactivity with rrCINC-2 beta, recombinant human (rh) GRO alpha, rhGRO beta, rhGRO gamma is observed and 5% cross-reactivity with rrCINC-1 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant rat CXCL2/GRO beta /MIP‑2/CINC‑3
Ser32-Asn100
Accession # P30348

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.20 EU per 1 μg of the antibody by the LAL method.

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Rat CXCL2/GRO beta /MIP‑2/CINC‑3 (Catalog # 525-C3)

Neutralization

Measured by its ability to neutralize CXCL2/GRO beta /MIP‑2/CINC‑3-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CXCR2. The Neutralization Dose (ND₅₀) is typically 0.5-2.5 µg/mL in the presence of 0.1 µg/mL Recombinant Rat CXCL2/GRO beta /MIP‑2/CINC‑3.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization

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Chemotaxis Induced by CXCL2/CINC‑3 and Neutralization by Rat CXCL2/CINC‑3 Antibody. Recombinant Rat CXCL2/CINC-3 (Catalog # 525-C3) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Rat CXCL2/CINC-3 (0.1 µg/mL) is neutralized (green line) by increasing concentrations of Rat CXCL2/CINC-3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF525). The ND₅₀ is typically 0.5-2.5 µg/mL.

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Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CXCL2/GRO beta/MIP-2/CINC-3

Cytokine-induced neutrophil chemoattractant 3 (CINC-3) has been found to be expressed by cytokine-stimulated rat alveolar macrophages and fibroblasts. Based on its protein and DNA sequences, CINC-3 (also known as rat MIP-2, GRO beta and CXCL2) is a member of the alpha (CXC) subfamily of chemokines.

CINC-3 cDNA encodes a 100 amino acid (aa) residue precursor protein with a 31 aa signal peptide that is cleaved to yield a 69 aa mature secreted protein. The protein sequence of rat CINC-3 shares approximately 88% identity with murine MIP-2. Characteristic of ELR containing CXC chemokines, CINC-3 is known to be a potent chemotactic factor for rat neutrophils in vitro and in vivo. On the basis of cross-desensitization results of the various CINC proteins, it has been postulated that rat neutrophils have at least two classes of CINC receptors: a class of CINC-3 specific receptors as well as a second common receptor shared by all CINCs.

References

Nakagawa, H. et al. (1994) Biochem. J. 301:545.
Huang, S. et al. (1992) Am. J. Pathol. 141:981.

Entrez Gene IDs

2920 (Human); 20310 (Mouse); 114105 (Rat)

Alternate Names

chemokine (C-X-C motif) ligand 2; CINC-2a; CINC3; CINC-3; C-X-C Motif Chemokine 2; CXCL2; GRO beta; GRO2 oncogene; GRO2; GROb; Gro-beta; Growth-regulated protein beta; Macrophage inflammatory protein 2-alpha; melanoma growth stimulatory activity beta; MGSA beta; MGSA-b; MGSA-beta; MIP2; MIP-2; MIP2A; MIP-2a; MIP2-alpha; SCYB2

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Citation for Rat CXCL2/GRO beta /MIP-2/CINC-3 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

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