CXCL7, also known as Thymus Chemokine-1, is a member of the alpha (CXC) subfamily of chemokines. It functions as a chemoattractant for neutrophils, macrophages and T cells that express CXCR2/IL-8 R beta. Rat CXCL7 cDNA encodes a 111 amino acid (aa) precursor protein. Rat CXCL7 shares 72% aa sequence identity with mouse CXCL7.
Rat CXCL7/Thymus Chemokine-1 Antibody (186701)
R&D Systems | Catalog # MAB1116
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Key Product Details
Species Reactivity
Rat
Applications
Western Blot, ELISA Capture (Matched Antibody Pair), Neutralization
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 186701
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Product Specifications
Immunogen
E. coli-derived recombinant rat CXCL7/Thymus Chemokine-1
Ile46-Ile107
Accession # NP_714943
Ile46-Ile107
Accession # NP_714943
Specificity
Detects rat CXCL7/Thymus Chemokine‑1 in ELISAs and Western blots. In sandwich ELISAs, no cross-reactivity or interference with recombinant human CXCL5, recombinant mouse CXCL7/Thymus Chemokine-1, recombinant rat (rr) CXCL1/CINC1, rrCXCL2/CINC3, rrCXCL3/CINC-2 alpha, rrCXCL3/CINC-2 beta, or rrCXCL5 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Scientific Data Images for Rat CXCL7/Thymus Chemokine-1 Antibody (186701)
Chemotaxis Induced by CXCL7/Thymus Chemokine‑1 and Neutralization by Rat CXCL7/Thymus Chemokine‑1 Antibody.
Recombinant Rat CXCL7/Thymus Chemokine-1 (Catalog # 1116-CK) chemo-attracts the BaF3 mouse pro-B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Rat CXCL7/ Thymus Chemokine-1 (15 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Rat CXCL7/ Thymus Chemokine-1 Monoclonal Antibody (Catalog # MAB1116). The ND50 is typically 4-20 µg/mL.Applications for Rat CXCL7/Thymus Chemokine-1 Antibody (186701)
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Recombinant Rat CXCL7/Thymus Chemokine‑1 (Catalog # 1116-CK)
Sample: Recombinant Rat CXCL7/Thymus Chemokine‑1 (Catalog # 1116-CK)
Neutralization
Measured by its ability to neutralize CXCL7/Thymus Chemokine‑1-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CXCR2. The Neutralization Dose (ND50) is typically 4-20 µg/mL in the presence of 15 ng/mL Recombinant Rat CXCL7/Thymus Chemokine‑1.
Rat Thymus Chemokine-1 Sandwich Immunoassay
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: CXCL7/Thymus Chemokine-1
Alternate Names
Thymus Chemokine1
Gene Symbol
PPBP
UniProt
Additional CXCL7/Thymus Chemokine-1 Products
Product Documents for Rat CXCL7/Thymus Chemokine-1 Antibody (186701)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Rat CXCL7/Thymus Chemokine-1 Antibody (186701)
For research use only
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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Associated Pathways
