Rat IL-17/IL-17A Alexa Fluor® 488-conjugated Antibody

Applications

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Preparation and Storage

Background: IL-17/IL-17A

Rat IL-17A/F is an approximately 37 kDa, secreted, disulfide-linked glycosylated heterodimeric protein comprised of two members of the IL-17 family of cytokines, IL-17A and IL-17F (1, 2). Members of this family demonstrate a structural motif termed a cysteine knot that also characterizes a large superfamily of growth factors such as TGF-beta (1). Although most cysteine knot superfamily members use three intrachain disulfide bonds to create a knot, IL-17 family molecules generate the same structural form with only two disulfide links (1, 3-5). Mature rat IL-17A and IL-17F share 60% and 59% amino acid sequence identity with human and 61% and 90% with mouse IL-17A and IL-17F, respectively. They also share 50% aa sequence identity with each other (1). IL-17A/F heterodimer and the IL-17A and IL-17F homodimers are produced by IL-23 activated Th17 cells (1, 6-10, 14). The widely expressed receptors IL-17 RA and IL-17 RC form a heterodimer for the binding of
IL-17A and IL-17F, as well as the heterodimeric IL-17A/F (6, 11, 12). IL-17A/F is a biologically active protein that induces chemokine production and airway neutrophilia with intermediate potency between IL-17A (most potent) and IL-17F (least potent) (7, 12). It is up-regulated in immune cells during inflammatory arthritis and contributes to disease severity (13).

Product Datasheets

Related Research Areas

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