Rat M-CSF Antibody

Catalog #: MAB5364 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB5364
MAB5364-SP

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Product Details

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Rat M-CSF Antibody Summary

Species Reactivity

Rat

Specificity

Detects rat M-CSF in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse M‑CSF and recombinant human M‑CSF is observed.

Source

Monoclonal Mouse IgG₁ Clone # 427406

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant rat M‑CSF
Glu33-Glu261
Accession # Q8JZQ0

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Rat M-CSF

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: M-CSF

M-CSF, also known as CSF-1, is a four-alpha −helical-bundle cytokine that is essential for survival, proliferation and differentiation of macrophages and osteoclast progenitors. M-CSF is produced by fibroblasts, activated macrophages, endometrial secretory epithelium, bone marrow stromal cells and activated endothelial cells. Full-length rat M-CSF transcripts encode a 534 amino acid (aa) type I transmembrane mature protein. In humans this encodes a 70 kDa protein that forms covalent homodimers. Human 22 kDa, 43 kDa and proteoglycan-modified 200 kDa secreted forms and 34 kDa transmembrane forms may also occur in rats. Mature rat M-CSF shares 89% and 74% aa identity with mouse and human M-CSF, respectively, within the first 229 aa.

Long Name

Macrophage Colony Stimulating Factor

Entrez Gene IDs

1435 (Human); 12977 (Mouse)

Alternate Names

colony stimulating factor 1 (macrophage); CSF1; CSF-1; Lanimostim; macrophage colony stimulating factor; macrophage colony-stimulating factor 1; MCSF; M-CSF; MCSFlanimostim; MGC31930