Raw 264.7 Whole Cell - T0901317 treated Whole Cell Lysate

Novus Biologicals | Catalog # NBP1-30158

Novus Biologicals
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Key Product Details

Species

Mouse

Applications

Western Blot

Product Specifications

Application Notes

This lysate is prepared as a positive control for separation by SDS-PAGE and subsequent Western blot analysis. Lysates are prepared in denaturing buffer WITHOUT reducing agents (i.e. 2-mercaptoethanol (BME) or dithiothreitol (DTT)). If reducing conditions are desired, add a reducing agent prior to heating. Heat lysate to 95C for 5 minutes and rapidly cool. The recommended loading amount is 0.01-0.03 mg per lane.

Localization

Macrophage

Type

Cell

Tissue

Blood Lymph

Protein State

Denatured

Subcellular Fraction

Whole

Scientific Data Images for Raw 264.7 Whole Cell - T0901317 treated Whole Cell Lysate

Western Blot: Raw 264.7 Whole CellT0901317 treated Whole Cell Lysate [NBP1-30158]

Western Blot: Raw 264.7 Whole CellT0901317 treated Whole Cell Lysate [NBP1-30158]

Western Blot: Raw 264.7 Whole Cell Lysate [NBP1-30158] - Analysis of ABCA1, using NB400-105. 25 ug of whole cell lysate of RAW 264.7 cells either treated with T0901317 (NBP1-30158 lane 1) or untreated (NBP1-30157 lane 2).

Formulation, Preparation, and Storage

Formulation

Standard RIPA buffer including protease and phosphatase inhibitors and 0.1% SDS diluted in 1X lithium dodecyl sulfate (LDS) sample buffer with bromophenol blue and 40-70% glycerol, pH 8.4. BME has not been added.

Concentration

1.0 mg/ml

Shipping

The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store at -80C. Avoid freeze-thaw cycles.

Background: Raw 264.7 Whole Cell - T0901317 treated

T0901317 is an LXR agonist which acts to upregulate ABCA1 gene expression and promotes reverse cholesterol transport from macrophages.

Additional Raw 264.7 Whole Cell - T0901317 treated Products

Product Documents for Raw 264.7 Whole Cell - T0901317 treated Whole Cell Lysate

Certificate of Analysis

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Product Specific Notices for Raw 264.7 Whole Cell - T0901317 treated Whole Cell Lysate

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Citations for Raw 264.7 Whole Cell - T0901317 treated Whole Cell Lysate

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