Recombinant Cynomolgus/Rhesus IL-21R Fc Chimera Protein, CF

Name: Recombinant Cynomolgus/Rhesus IL-21R Fc Chimera Protein, CF
Brand: R&D Systems
SKU: 10482-R2
Price: 491 USD
Availability: InStock

R&D Systems | Catalog # 10482-R2

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Product Specifications
Scientific Data
Preparation & Storage
Calculators
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

R&D Systems CHO-derived Recombinant Cynomolgus/Rhesus IL-21R Fc Chimera Protein (10482-R2)
Quality control testing to verify active proteins with lot specific assays by in-house scientists
All R&D Systems proteins are covered with a 100% guarantee

Source

CHO

Accession Number

XP_005591578.1

Structure / Form

Disulfide-linked homodimer

Applications

Bioactivity

View all IL-21R Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived IL-21R protein

Cynomolgus Monkey/Rhesus Macaque IL‑21R (Cys20-Pro236) Accession # XP_005591578.1	IEGRMD	Human IgG₁ (Pro100-Lys330)
N-terminus		C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Cys20

Predicted Molecular Mass

52 kDa

SDS-PAGE

60-80 kDa, under reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human IL-21 Protein (Catalog # 8879-IL) is immoblized at 2.5 µg/mL (100 µL/well), the concentration of Recombinant Cynomolgus Monkey/Rhesus Macaque IL-21R Fc Chimera (Catalog # 10482-R2) that produces a 50% optimal binding response is found to be 0.6-3.6 µg/mL.

Scientific Data Images for Recombinant Cynomolgus/Rhesus IL-21R Fc Chimera Protein, CF

Recombinant Cynomolgus/Rhesus IL-21R Fc Chimera Protein Binding Activity

When Recombinant Human IL-21 Protein (8879-IL) is immobilized at 2.5 μg/mL (100 μL/well), the concentration of Recombinant Cynomolgus Monkey/Rhesus Macaque IL-21R Fc Chimera (10482-R2) that produces a 50% optimal binding response is found to be 0.6-3.6 μg/mL.

Recombinant Cynomolgus/Rhesus IL-21R Fc Chimera Protein SDS-PAGE

2 μg/lane of Recombinant Cynomolgus Monkey/Rhesus Macaque IL-21R Fc Chimera Protein (Catalog # 10482-R2) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 60-80 kDa and 110-160 kDa, respectively.

Formulation, Preparation, and Storage

10482-R2

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: IL-21R

Interleukin-21 Receptor (IL-21 R) is a type I transmembrane glycoprotein within the class I cytokine receptor family (1). IL-21 R associates with the common gamma chain ( gamma _c) which is also a component of the receptors for IL-2, IL-4, IL-7, IL-9, IL-13, and IL-15 (2, 3). Based on its similarity with human IL-21 R, mature cynomolgus/rhesus IL-21 R is predicted to consist of a 213 amino acid (aa) extracellular domain (ECD), a fibronectin type III domain, and a WSxWS motif, followed by a 21 aa transmembrane domain and a 253 aa cytoplasmic domain with a Box 1 motif, a kinase domain, and several sites for tyrosine phosphorylation (4, 5). Within the ECD, cynomolgus/rhesus IL-21 R shares 98% aa identity with human IL-21 R. IL-21 R is expressed mainly on B cells (highest on mature, activated, follicular and germinal center B cells), NK cells, and activated T cells, but is also found on dendritic cells, alternatively activated macrophages, intestinal lamina propria fibroblasts and epithelial cells, and keratinocytes (1, 4, 5). Both IL-21 and IL-4 are necessary for efficient B cell IgG1 production and normal germinal center architecture (6). B cell IL-21 R engagement induces Blimp-1 (which mediates plasma cell differentiation) and is important for memory responses (7, 8). IL-21 R engagement enhances NK cell mediated cytotoxic activity and IFN-gamma production (4, 9), control of viral infection and tumor growth by CD8⁺ T cells (10), development of regulatory T cells (11), IL-23 responsiveness of encephalitogenic Th17 cells (12), but suppresses the accumulation of IL-17 secreting gamma delta T cells in the airway (13). IL-21 R expression is often upregulated in allergic skin inflammation, systemic lupus erythematosus and diffuse large B cell lymphoma (DLBCL) (14, 15).