Recombinant Human Aminopeptidase PILS/ARTS1 Protein, CF

R&D Systems | Catalog # 2334-ZN

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Key Product Details

  • R&D Systems NS0-derived Recombinant Human Aminopeptidase PILS/ARTS1 Protein (2334-ZN)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

NS0

Accession Number

EAW96077

Applications

Enzyme Activity
View all Aminopeptidase PILS/ARTS1 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human Aminopeptidase PILS/ARTS1 protein
Ala37-Met941, with a C-terminal 10-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala37

Predicted Molecular Mass

105 kDa

SDS-PAGE

108 kDa, reducing conditions

Activity

Measured by its ability to cleave the fluorogenic peptide substrate, Leu-AMC.
The specific activity is >125 pmol/min/µg, as measured under the described conditions.

Formulation, Preparation, and Storage

2334-ZN
Formulation Supplied as a 0.2 μm filtered solution in Tris and NaCl.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: Aminopeptidase PILS/ARTS1

The name of Aminopetidase PILS (Puromycin-Insensitive Leucyl-Specific) describes the two basic properties of this zinc metalloprotease in vitro (1). Also known as ALAP (Adipocyte-derived Leucin AminoPeptidase), type 1 tumor necrosis factor receptor (TNFR) shedding aminopeptidase regulator and ER aminopeptidase ERAP1 or ERAAP, it is encoded by the ARTS1 gene (2-4). Aminopeptidase PILS has been identified to regulate antigen presentation, promote TNFR1 ectodomain shedding and associate with hypertension (2-5).

References

  1. Schomburg, L. (2004) in Handbook of Proteolytic Enzymes (ed. Barrett, et al.) pp. 311, Academic Press, San Diego.
  2. Cui, X. et al. (2002) J. Clin. Invest. 110:515.
  3. York, I.A. et al. (2002) Nat. Immunol. 3:1177.
  4. Serwold, T. et al. (2002) Nature 419:480.
  5. Yamamoto, N. et al. (2002) Hum. Mutat. 19:251.

Long Name

Aminopeptidase Puromycin-insensitive Leucyl-specific

Alternate Names

A-LAP, ARTS1, ERAAP1, ERAP1, PILS-AP

Entrez Gene IDs

51752 (Human); 80898 (Mouse); 102146570 (Cynomolgus Monkey)

Gene Symbol

ERAP1

UniProt

EAW96077

Additional Aminopeptidase PILS/ARTS1 Products

Product Documents for Recombinant Human Aminopeptidase PILS/ARTS1 Protein, CF

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Recombinant Human Aminopeptidase PILS/ARTS1 Protein, CF

For research use only

Related Research Areas

Metalloproteases and Regulators

Citations for Recombinant Human Aminopeptidase PILS/ARTS1 Protein, CF

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Protocols

View specific protocols for Recombinant Human Aminopeptidase PILS/ARTS1 Protein, CF (2334-ZN):

Materials
  • Assay Buffer: 25 mM Tris, pH 8.0
  • Recombinant Human Aminopeptidase PILS/ARTS1 (rhARTS1) (Catalog # 2334-ZN)
  • Substrate: Leu-AMC (Bachem, Catalog # I-1240)
  • F16 Black Maxisorp Plate (Nunc, Catalog # 475515)
  • Fluorescent Plate Reader (Model: SpectraMax Gemini EM by Molecular Devices) or equivalent
  1. Dilute rhARTS1 to 2 µg/mL in Assay Buffer.
  2. Dilute Substrate to 600 µM in Assay Buffer.
  3. Load 50 µL of 2 µg/mL rhARTS1 into the plate, and start the reaction by adding 50 µL of 600 µM Substrate. Include a Substrate Blank containing Assay Buffer and Substrate.
  4. Read at excitation and emission wavelengths of 380 nm and 460 nm (respectively), in kinetic mode for 5 minutes.
  5. Calculate specific activity:

     Specific Activity (pmol/min/µg) =

 Adjusted Vmax* (RFU/min) x Conversion Factor** (pmol/RFU)
amount of enzyme (µg)

     *Adjusted for Substrate Blank
     **Derived using calibration standard 7-amino, 4-Methyl Coumarin (Sigma, Catalog # A-9891).

Per Well:
  • rhARTS1: 0.1 µg
  • Substrate: 300 µM

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