Recombinant Human CCL28 Protein

R&D Systems | Catalog # 717-VC

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Key Product Details

  • R&D Systems E. coli-derived Recombinant Human CCL28 Protein (717-VC)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

E. coli

Accession Number

Q9NRJ3

Applications

Bioactivity
View all CCL28 Proteins and Enzymes »

Product Specifications

Source

E. coli-derived human CCL28 protein
Ile23-Tyr127

Purity

>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ile23

Predicted Molecular Mass

12 kDa

Activity

Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with human CCR10.
The ED50 for this effect is 0.4-2.0 µg/mL.

Scientific Data Images for Recombinant Human CCL28 Protein

Recombinant Human CCL28 Protein Bioactivity

Recombinant Human CCL28 Protein Bioactivity

Recombinant Human CCL28 (Catalog # 717-VC) chemoattracts the BaF3 mouse pro-B cells transfected with mouse CCR10. The ED50 for this effect is 0.4‑2.0 μg/mL.
Recombinant Human CCL28 Protein SDS-PAGE

Recombinant Human CCL28 Protein SDS-PAGE

1 μg/lane of Recombinant Human CCL28 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 14 kDa.

Formulation, Preparation, and Storage

Carrier Free
What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Carrier: 717-VC
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 25 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Carrier Free: 717-VC/CF
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CCL28

Human CCL28 (CC chemokine ligand 28) is a novel CC chemokine identified by TBLASTN searches of the Human Genome Systems (HGS) and Genbank dbEst database using a human chemokine consensus sequence. Human CCL28 cDNA encodes a 127 amino acid (aa) residue precursor protein with a putative 22 aa residue signal peptide that is cleaved to produce the 105 aa residue mature protein. Human and mouse CCL28 are highly conserved, sharing 83% aa identity in their mature regions. Among CC chemokines, CCL28 shares the most homology with CCL27/CTACK. The mouse CCL28 gene has been mapped to the distal region of chromosome 13. Human and mouse CCL28 RNA expression was found to be highest in normal and pathologic colon with the protein being expressed by epithelial cells. Human CCL28 RNA was also present in normal and asthmatic lung tissues. The receptor for CCL28 has been identified as the CCR10 (GPR2 orphan receptor) which is also the receptor for CCL27/CTACK.

References

  1. Wang, W. et al. (2000) J. Biol. Chem. 275:22313.

Alternate Names

CCL28, MEC, VIC

Entrez Gene IDs

56477 (Human); 56838 (Mouse)

Gene Symbol

CCL28

UniProt

Q9NRJ3

Additional CCL28 Products

Product Documents for Recombinant Human CCL28 Protein

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS 717-VC (with carrier)
Download SDS 717-VC/CF (carrier free)

Product Specific Notices for Recombinant Human CCL28 Protein

For research use only

Related Research Areas

Chemokines and Receptors

Citations for Recombinant Human CCL28 Protein

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