CXCL5, also known as epithelial cell-derived neutrophil-activating peptide (ENA-78), is an 8 kDa proinflammatory member of the CXC subfamily of chemokines. Its Glu-Leu-Arg (ELR) motif confers angiogenic properties and distinguishes it from ELR-CXC chemokines which are angiostatic (1-3). Human CXCL5 shares 57% amino acid (aa) sequence identity with mouse and rat CXCL5. Among other human ELR+ chemokines, it shares 77% aa sequence identity with CXCL6/GCP-2 and 35%-51% with CXCL1/GRO alpha, CXCL2/GRO beta, CXCL3/GRO gamma, CXCL7/NAP-2, and CXCL8/IL-8. Inflammatory stimulation up-regulates CXCL5 production in multiple hematopoietic cell types, fibroblasts, endothelial cells, and vascular smooth muscle cells. In vivo, CXCL5 is elevated at sites of inflammation and pulmonary fibrosis where it promotes neutrophil infiltration and activation as well as angiogenesis (3-6). Its up-regulation contributes to increased vascularization, tumor growth, and metastasis in many cancers (6-9). Full length CXCL5 (78 aa) is trimmed at the N-terminus by cathepsin G and chymotrypsin to ENA-74 (74 aa) and ENA-70 (70 aa), with the shortened forms showing increased potency relative to full length CXCL5 (10, 11). CXCL5 exerts its effects primarily through interactions with CXCR2 (6, 12). It also binds duffy antigen receptor for chemokines (DARC), which can limit CXCR2-mediated responses (13, 14).
Recombinant Human CXCL5/ENA-78 Protein
R&D Systems | Catalog # 254-XB
Key Product Details
- R&D Systems E. coli-derived Recombinant Human CXCL5/ENA-78 Protein (254-XB)
- Quality control testing to verify active proteins with lot specific assays by in-house scientists
- All R&D Systems proteins are covered with a 100% guarantee
Source
Accession Number
Applications
Product Specifications
Source
Ala37-Asn114
Purity
Endotoxin Level
N-terminal Sequence Analysis
Predicted Molecular Mass
SDS-PAGE
Activity
The ED50 is 3-15 ng/mL
Reviewed Applications
Read 2 reviews rated 4.5 using 254-XB in the following applications:
Formulation, Preparation, and Storage
Carrier Free
What does CF mean?CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
What formulation is right for me?In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
Carrier: 254-XB
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS containing BSA as carrier protein |
| Reconstitution | Reconstitute at 100 μg/mL in PBS containing at least 0.1% human or bovine serum albumin. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Carrier Free: 254-XB/CF
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 100 μg/mL in PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Calculators
Background: CXCL5/ENA-78
References
- Strieter, R.M. et al. (2005) Cytokine Growth Factor Rev. 16:593.
- Balestrieri, M.L. et al. (2008) Cardiovasc. Res. 78:250.
- Walz, A.. et al. (1991) J. Exp. Med. 174:1355.
- Strieter, R.M. et al. (1992) Immunol. Invest. 21:549.
- Koch, A.E. et al. (1994) J. Clin. Invest. 94:1012.
- Begley, L.A. et al. (2008) Neoplasia 10:244.
- Vandercappellen, J. et al. (2008) Cancer Lett. 267:226.
- Arenberg, D.A. et al. (1998) J. Clin. Invest. 102:465.
- Miyazaki, H. et al. (2006) Cancer Res. 66:4279.
- Wuyts, A. et al. (1999) Eur. J. Biochem. 260:421.
- Nufer, O. et al. (1999) Biochemistry 38:636.
- Ahuja, S.K. and P.M. Murphy (1996) J. Biol. Chem. 271:20545.
- Kashiwazaki, M. et al. (2003) Int. Immunol. 15:1219.
- Horton, L.W. et al. (2007) Cancer Res. 67:9791.
Alternate Names
Entrez Gene IDs
Gene Symbol
UniProt
Additional CXCL5/ENA-78 Products
Product Documents for Recombinant Human CXCL5/ENA-78 Protein
Product Specific Notices for Recombinant Human CXCL5/ENA-78 Protein
For research use only
Related Research Areas
Citations for Recombinant Human CXCL5/ENA-78 Protein
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Application: Immunoassay StandardVerified Customer | Posted 08/23/2018Used with MAB654 in an immunoassay.
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Application: In vitro bioactivity in cell cultureVerified Customer | Posted 08/20/2018
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