Recombinant Human Cyr61/CCN1 Fc Chimera Protein, CF

Catalog # Availability Size / Price Qty

SAVE +FREE SHIPPING with ACADEMIA23 (See Promo Details)

R&D Systems Recombinant Proteins and Enzymes
1 Image
Product Details
Citations (5)
Supplemental Products
Reviews (2)

Recombinant Human Cyr61/CCN1 Fc Chimera Protein, CF Summary

Product Specifications

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
Measured by its ability to mediate Balb/3T3 mouse embryonic fibroblast cell adhesion. Ball, D.K. et al. (2003) Reproduction 125:271. Recombinant Human Cyr61/CCN1 Fc Chimera immobilized at 1.5 μg/mL, 100 μL/well, will mediate >25% BALB/3T3 cell adhesion
(3 x 104 cell/well added).
Chinese Hamster Ovary cell line, CHO-derived human Cyr61/CCN1 protein
Human Cyr61
Accession # O00622
N-terminus C-terminus
Accession #
N-terminal Sequence
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
66.3 kDa
70-75 kDa, reducing conditions

Product Datasheets

You must select a language.



Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.


Formulation Lyophilized from a 0.2 μm filtered solution in PBS and NaCl.
Reconstitution Reconstitute at 250 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Background: Cyr61/CCN1

Cyr61, also known as CCN1, is a 40-45 kDa matricellular glycoprotein that plays an important role in cellular adhesion and migration (1). Cyr61 consists of an IGFBP domain, a VWF type C domain, a TSP type I domain, and a cysteine knot domain (2). Mature human Cyr61 shares 93% amino acid sequence identity with mouse and rat Cyr61. It is widely expressed during development and in adult tissues (2, 3). Cyr61 associates with the extracellular matrix (ECM) and with many cell surface molecules including Integrins alpha V beta 3, alpha V beta 5, alpha M beta 2, and alpha 6 beta 1, Syndecan-4, and heparan sulfate proteoglycans (1, 3). Cyr61 mediates the adhesion and migration of multiple cell types and also promotes vascular endothelial cell tubule formation (4-6). Plasmin cleavage of ECM-bound Cyr61 releases a 28 kDa N-terminal fragment which retains the ability to promote endothelial cell migration (7). Cyr61 exhibits both tumorigenic and tumor suppressor properties. It is up-regulated and promotes tumorigenesis, angiogenesis, and metastasis in breast, renal, gastric, squamous cell, and colorectal carcinomas as well as in glioma (8-12). In contrast, when
down-regulated, it suppresses tumor growth in endometrial, hepatic, and non-small cell lung cancers (8, 13, 14). Cyr61 is also up-regulated in injured skin and bone where it induces the expression of growth factors, cytokines, proteases, and integrins involved in wound repair (15, 16).

  1. Chen, Y. and X.Y. Du (2007) J. Cell. Biochem. 100:1337.
  2. Jay, P. et al. (1997) Oncogene 14:1753.
  3. Feng, P. et al. (2008) Int. J. Biochem. Cell Biol. 40:98.
  4. Chen, N. et al. (2000) J. Biol. Chem. 275:24953.
  5. Leu, S.-J. et al. (2002) J. Biol. Chem. 277:46248.
  6. Schutze, N. et al. (2007) BMC Cell Biol. 8:45.
  7. Pendurthi, U.R. et al. (2005) Cancer Res. 65:9705.
  8. O'Kelly, J. et al. (2008) Int. J. Oncol. 33:59.
  9. Chintalapudi, M.R. et al. (2008) Carcinogenesis 29:696.
  10. Xie, D. et al. (2004) Cancer Res. 64:1987.
  11. Lin, M.-T. et al. (2007) J. Biol. Chem. 282:34594.
  12. Monnier, Y. et al. (2008) Cancer Res. 68:7323.
  13. Tong, X. et al. (2001) J. Biol. Chem. 276:47709.
  14. Chien, W. et al. (2004) J. Biol. Chem. 279:53087.
  15. Hadjiargyrou, M. et al. (2000) J. Bone Miner. Res.15:1014.
  16. Chen, C.-C. et al. (2001) J. Biol. Chem. 276:47329.
Long Name
Cysteine-Rich, Angiogenic Inducer 61
Entrez Gene IDs
3491 (Human); 16007 (Mouse); 83476 (Rat)
Alternate Names
CCN family member 1; CCN1; CCN1IGF-binding protein 10; Cyr61; Cysteine-rich angiogenic inducer 61; cysteine-rich, angiogenic inducer, 61; GIG1; GIG1cysteine-rich heparin-binding protein 61; IBP-10; IGFBP-10; IGFBP10cysteine-rich, anigogenic inducer, 61; Insulin-like growth factor-binding protein 10; protein CYR61; Protein GIG1

Citations for Recombinant Human Cyr61/CCN1 Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
Filter your results:

Filter by:

  1. Astragaloside IV improves pulmonary arterial hypertension by increasing the expression of CCN1 and activating the ERK1/2 pathway
    Authors: Y Liu, BL Tang, ML Lu, HX Wang
    Journal of Cellular and Molecular Medicine, 2023-02-10;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  2. CCN1 interacts with integrins to regulate intestinal stem cell proliferation and differentiation
    Authors: JH Won, JS Choi, JI Jun
    Nature Communications, 2022-06-03;13(1):3117.
    Species: Mouse
    Sample Types: Organoids
    Applications: Bioassay
  3. CCN1/CYR61-mediated meticulous patrolling by Ly6Clow monocytes fuels vascular inflammation
    Proc Natl Acad Sci USA, 2016-08-01;113(33):E4847-56.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. L-Proline induces a mesenchymal-like invasive program in embryonic stem cells by remodeling H3K9 and H3K36 methylation.
    Authors: Comes, Stefania, Gagliardi, Miriam, Laprano, Nicola, Fico, Annalisa, Cimmino, Amelia, Palamidessi, Andrea, De Cesare, Dario, De Falco, Sandro, Angelini, Claudia, Scita, Giorgio, Patriarca, Eduardo, Matarazzo, Maria R, Minchiotti, Gabriell
    Stem Cell Reports, 2013-10-10;1(4):307-21.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  5. Sclerostin binds and regulates the activity of cysteine-rich protein 61.
    Authors: Craig TA, Bhattacharya R, Mukhopadhyay D, Kumar R
    Biochem. Biophys. Res. Commun., 2009-12-31;392(1):36-40.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay


No product specific FAQs exist for this product, however you may

View all Proteins and Enzyme FAQs

Reconstitution Buffers

Reviews for Recombinant Human Cyr61/CCN1 Fc Chimera Protein, CF

Average Rating: 5 (Based on 2 Reviews)

5 Star
4 Star
3 Star
2 Star
1 Star

Have you used Recombinant Human Cyr61/CCN1 Fc Chimera Protein, CF?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Filter by:

Recombinant Human Cyr61/CCN1 Fc Chimera Protein, CF
By Anonymous on 05/20/2019
Application: Enzymatic activity in vitro

Recombinant Human Cyr61/CCN1 Fc Chimera Protein, CF
By Anonymous on 09/30/2018
Application: In vitro bioactivity in cell culture