Recombinant Human DLL4 Fc Chimera Protein, CF

R&D Systems | Catalog # BT-DLL4

Analyzed by SEC-MALS
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Key Product Details

Source

CHO

Structure / Form

Disulfide-linked homodimer

Applications

Bioactivity
View all DLL4 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human DLL4 protein
Human DLL4
(Ser27-Pro524)
Accession # Q9NR61.1		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE with quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ser 27

Predicted Molecular Mass

81 kDa

SDS-PAGE

89-98 kDa, under reducing conditions.

Activity

Measured by its binding ability in a functional ELISA.
Recombinant Human DLL4 Fc Chimera (Catalog # BT-DLL4) binds to Recombinant Human Notch-1 Fc Chimera (Catalog # 3647-TK) with an ED50 of 2.00-20.0 ng/mL.

Scientific Data Images for Recombinant Human DLL4 Fc Chimera Protein, CF

Recombinant Human DLL4 Fc Chimera Protein SEC-MALS.

Recombinant Human DLL4 Fc Chimera Protein (Catalog # BT-DLL4) has a molecular weight (MW) of 168-185 kDa as analyzed by SEC-MALS, suggesting that this protein is a homodimer. MW may differ from predicted MW due to post-translational modifications (PTMs) present (i.e. Glycosylation).

Equivalent Binding Activity of GMP and RUO grades of Recombinant Human DLL4.

Equivalent binding activity of GMP (BT-DLL4-GMP) and RUO (Catalog # BT-DLL4) grades of RecombinantHuman DLL4 as measured in a functional ELISA with Recombinant Human Notch-1 Protein (orange and red, respectively).

Recombinant Human DLL4 Fc Chimera Protein Binding Activity.

In a functional ELISA, Recombinant Human DLL4 Fc Chimera Protein (Catalog # BT-DLL4) binds to Recombinant Human Notch-1 Fc Chimera (3647-TK) with an ED50 of 2.00-20.0 ng/mL.

Recombinant Human DLL4 Fc Chimera Protein SDS-PAGE.

2 μg/lane of Recombinant Human DLL4 Fc Chimera Protein (Catalog # BT-DLL4) was resolved with SDS-PAGE under reducing (R) condition and visualized by Coomassie® Blue staining, showing bands at 89-98 kDa.

Formulation, Preparation, and Storage

BT-DLL4
Formulation Lyophilized from a 0.2 μm filtered solution in HBS and Tween®-80 with Trehalose.
Reconstitution

Reconstitute the 20 μg size at 100 μg/mL in water. Reconstitute all other sizes at 500 μg/mL in water.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: DLL4

Delta-like protein 4 (DLL4) is a type I membrane protein belonging to the Delta/Serrate/Lag2 (DSL) family of Notch ligands (1). Notch signaling is an evolutionarily conserved pathway that controls cell fate and is required in multiple developmental processes including vascular development, hematopoiesis, somatogenesis, myogenesis, and neurogenesis (2-4). Dysregulation in the Notch pathway is associated with various human diseases. In mammals, four Notch homologs (Notch 1 to 4) and five ligands (DLL 1, 3 and 4, Jagged 1 and 2) have been identified. Notch ligands are transmembrane proteins with a DSL motif necessary for Notch binding, tandem EGF repeats, a transmembrane region and a short intracellular domain (ICD). Notch ligands are categorized into two subfamilies based on the presence of an extracellular cysteine-rich domain and insertions that interrupt some EGF repeats in the Jagged but not the Delta ligand family. Interactions of Notch receptors with their ligands results in reciprocal regulated intramembrane proteolysis (RIP) (4). RIP is a mechanism for transmembrane signal transduction that involves the sequential processing by a disintegrin metalloprotease (ADAM) and then by presenilin/ gamma secretase, resulting in shedding of the extracellular domains and the generation of the soluble ICD signaling fragments, respectively. The Notch ICD translocates to the nucleus and interacts with transcriptional coactivators, resulting in the transcription of target genes. The ICDs of the Notch ligands have also been shown to translocate to the nucleus where they may have a signaling function (5, 6). DLL4 is expressed highly and selectively within the arterial endothelium and has been shown to function as a ligand for Notch 1 and Notch 4. Human and mouse DLL4 share 86% amino acid sequence identity (1). The use of DLL4, in conjunction with VCAM1, has been shown as an effective method for generating hematopoietic progenitors and T cells from hPSCs in a serum- and feeder-free environment.

References

  1. Shutter, J.R. et al. (2000) Genes Dev. 14:1313.
  2. Iso, Tatsuya et al. (2002) Arterioscler. Thromb. Vasc. Biol. 23:543.
  3. Walker, L. et al. (2001) Stem Cells 19:543.
  4. Baron, M. (2002) Semin. Cell Dev. Biol. 14:113.
  5. Ikeuchi, T. and S.S. Sisodia (2003) J. Biol. Chem. 278:7751.
  6. Bland, C.E. et al. (2003) J. Biol. Chem. 278:13607.

Long Name

Delta-like 4

Alternate Names

delta 4, Delta 4 precursor, delta ligand 4, delta4, delta-like 4 (Drosophila), delta-like 4 homolog, delta-like 4 homolog (Drosophila), delta-like 4 protein, delta-like protein 4, Drosophila Delta homolog 4, hdelta2, MGC126344, notch ligand delta-2, notch ligand DLL4

Entrez Gene IDs

54567 (Human); 54485 (Mouse); 100152163 (Porcine)

Gene Symbol

DLL4

Additional DLL4 Products

Product Documents for Recombinant Human DLL4 Fc Chimera Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Human DLL4 Fc Chimera Protein, CF

For research use only

Related Research Areas

Cancer Stem Cell Markers
Notch Pathway

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Associated Pathways

Notch Signaling Pathways Notch Signaling Pathway Thumbnail