Recombinant Human Notch-1 Fc Chimera Protein, CF

R&D Systems | Catalog # 3647-TK

R&D Systems
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Key Product Details

  • R&D Systems NS0-derived Recombinant Human Notch-1 Fc Chimera Protein (3647-TK)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

NS0

Accession Number

P46531

Structure / Form

Disulfide-linked homodimer

Applications

Binding Activity
View all Notch-1 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human Notch-1 protein
Human Notch-1
(Ala19 - Gln526)
Accession # P46531		 IEGRMD Human IgG1
(Pro100 - Lys330)
N-terminus C-terminus

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.1 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala19

Predicted Molecular Mass

80.1 kDa (monomer)

SDS-PAGE

115-125 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
Immobilized rhNotch-1 Fc Chimera at 5 µg/mL (100 µL/well) can bind rrJagged-1/Fc Chimera with an apparent KD <5 nM in a functional ELISA.

Reviewed Applications

Read 3 reviews rated 4 using 3647-TK in the following applications:

Formulation, Preparation, and Storage

3647-TK
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution

Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Notch-1

Human Notch-1 is a 300 kDa type I transmembrane glycoprotein that is one of four human Notch homologues involved in developmental processes (1 - 3). Notch signaling is important for maintaining stem cells and inducing differentiation, especially in the nervous system and lymphoid tissues (2 - 4). Notch can specify binary cell fates; for example, promoting T- over B-cell development from a common precursor (2). More than 50% of human T-lineage acute lymphoblastic leukemia (T-ALL) have activating mutations of Notch1 (1, 5). Human Notch-1 is synthesized as a 2556 amino acid (aa) precursor that contains an 18 aa signal sequence, a 1718 aa extracellular domain (ECD) with 36 EGF-like repeats and three Lin-12/Notch repeats (LNR), a 23 aa transmembrane (TM) segment and a 785 aa cytoplasmic domain containing six ankyrin repeats, a glutamine-rich domain and a PEST sequence. The 11th and 12th EGF-like repeats bind ligands including Jagged and Delta-like families in humans (6). O-fucosylation by Fringe family members at a site within this region can inhibit the interaction of Notch with Jagged ligands, thereby promoting Delta-like ligand interactions (7). Notch-1 receptor undergoes post-translational furin-type proteolytic cleavage, forming a heterodimer through interaction of a hydrophobic area C-terminal to the LNR on the 1647 aa ligand-binding extracellular region with the 891 aa transmembrane/cytoplasmic portion (8, 9). Upon ligand binding, additional sequential proteolysis by TNF-converting enzyme (ADAM-17) and the presenilin-dependent gamma -secretase results in the release of the Notch intracellular domain (NICD) which translocates into the nucleus, activating transcription of Notch-responsive genes (10). Human Notch-1 ECD aa 19 - 526, including the first 13 EGF repeats, shows 91% aa identity with corresponding regions of mouse and rat, 89% with canine, and 79% with chicken Notch-1. This region also exhibits 60% aa identity with human Notch-2 and Notch-3.

References

  1. Ellisen, L. W. et al. (1991) Cell 66:649.
  2. Dumortier, A. et al. (2005) Int. J. Hematol. 82:277.
  3. Yoon, K. and N. Gaiano (2005) Nat. Neurosci. 8:709.
  4. Androutsellis-Theotokis, A. et al. (2006) Nature 442:823.
  5. Weng, A. P. et al. (2004) Science 306:269.
  6. Hambleton, S. et al. (2004) Structure 12:2173.
  7. Yang, L. et al. (2005) Mol. Biol. Cell 16:927.
  8. Sanchez-Irizarry, C. et al. (2004) Mol. Cell. Biol. 24:9265.
  9. Logeat, F. et al. (1998) Proc. Natl. Acad. Sci. USA 95:8108.
  10. Mumm, J.S. and R. Kopan (2000) Dev. Biol. 228:151.

Alternate Names

Notch1, TAN1

Entrez Gene IDs

4851 (Human); 25496 (Rat)

Gene Symbol

NOTCH1

UniProt

P46531

Additional Notch-1 Products

Product Documents for Recombinant Human Notch-1 Fc Chimera Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Human Notch-1 Fc Chimera Protein, CF

For research use only

Citations for Recombinant Human Notch-1 Fc Chimera Protein, CF

Customer Reviews for Recombinant Human Notch-1 Fc Chimera Protein, CF (3)

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  • Name: Jignesh Ganeshbhai Patel
    Application: Binding assay/Protein-protein interaction
    Verified Customer | Posted 09/10/2018
  • Name: Jignesh Patel
    Application: Enzymatic activity in vitro
    Verified Customer | Posted 09/10/2018
  • Name: Jignesh Patel
    Application: Binding assay/Protein-protein interaction
    Verified Customer | Posted 08/21/2018

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