ErbB2, also called Neu and Her2 (human epidermal growth factor receptor 2), is a type I membrane glycoprotein that is a member of the ErbB family of tyrosine kinase receptors. ErbB family members serve as receptors for the epidermal growth factor (EGF) family of growth factors. ErbB2 is widely expressed in epithelial cells and has also been found to be over-expressed in a large number of breast carcinomas. Among ErbB family members, ErbB2 is unique in that it has no identified ligands. Rather, ErbB2 heterodimerizes with the other members of the ErbB family (ErbB1 (EGFR), ErbB3, ErbB4) to form higher affinity signaling complexes. Because ErbB3 contains a defective kinase domain, the kinase domain of ErbB2 is responsible for initiating the tyrosine phosphorylation signal through the heterodimeric receptor. It has been found that a discrete three amino acid signal in the ErbB3 cytoplasmic domain is critical for transactivation of ErbB2. Interestingly, this same three amino acid signal has also been found in ErbB1 and ErbB4. Phosphoinositide 3-kinase has been shown to play a role in ErbB2 signal transduction. The cytoplasmic domain of ErbB2 has been shown to associate with beta-catenin and plakoglobin. Human ErbB2 consists of 1255 amino acids (aa) with a 21 aa signal sequence, a 631 aa extracellular domain, a 23 aa transmembrane region, and a 580 aa cytoplasmic domain. ErbB2 can be shed from the cell surface by proteolytic cleavage by an unidentified protease. ErbB2 appears to play roles in development, cancer, communication at the neuromuscular junction and regulation of cell growth and differentiation (1-10).
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Recombinant Human ErbB2/Her2 Fc Chimera Protein, CF
R&D Systems | Catalog # 1129-ER
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Key Product Details
- R&D Systems NS0-derived Recombinant Human ErbB2/Her2 Fc Chimera Protein (1129-ER)
- Quality control testing to verify active proteins with lot specific assays by in-house scientists
- All R&D Systems proteins are covered with a 100% guarantee
Source
NS0
Accession Number
Structure / Form
Disulfide-linked homodimer
Applications
Bioactivity
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Product Specifications
Source
Mouse myeloma cell line, NS0-derived human ErbB2/Her2 protein
| Human Erb B2 (Thr23-Thr652) Accession # NP_004439 |
IEGRMD | Human IgG1 (Pro100-Lys330) |
6 His-tag |
| N-terminus | C-terminus | ||
Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Thr23
Predicted Molecular Mass
96.8 kDa (monomer)
SDS-PAGE
125-130 kDa, reducing conditions
220-250 kDa, non-reducing conditions
220-250 kDa, non-reducing conditions
Activity
Measured by its ability to block anti-ErbB2 mediated inhibition of SK‑BR‑3 human breast cancer cell proliferation. Brodowicz, T. et al. (1997) Int. J. Cancer 73:875.
The ED50 for this effect is 0.3-1.2 μg/mL in the presence of 0.6 µg/mL Anti-Human ErbB2/Her2 Monoclonal Antibody
(Trastuzumab, Catalog #
MAB9589).
Reviewed Applications
Read 10 reviews rated 4.9 using 1129-ER in the following applications:
- Apoptosis assay (1 Review)
- Binding assay/Protein-protein interaction (2 Reviews)
- Cell Proliferation (1 Review)
- Characterization prior to use. (1 Review)
- ELISA Control (1 Review)
- Enzymatic activity in vitro (2 Reviews)
- Immunoassay Standard (1 Review)
- Media additive for protein or antibody production (1 Review)
Scientific Data Images for Recombinant Human ErbB2/Her2 Fc Chimera Protein, CF
Bioactivity of Human ErbB2 / Her2 Protein
1 μg/lane of Recombinant Human ErbB2 Fc Chimera was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing major bands at 125-130 kDa and 220-250 kDa, respectively.
Bioactivity of Human ErbB2 / Her2 Protein
Recombinant human ErB2 / Her2 (1129-ER) blocks anti-ErbB2 mediated inhibition of SK-BR-3 human breast cancer proliferation. The ED50 for this effect is 0.3-1.2 µg/mL in the presence of 0.6 µg/mL Anti-Human ErbB2/Her2 Monoclonal Antibody (Trastuzumab, MAB9589).
Formulation, Preparation, and Storage
1129-ER
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS.
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| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Calculators
Background: ErbB2/Her2
References
- Coussens, L. et. al. (1985) Science 230:1132.
- Yamamoto, T. et. al. (1986) Nature 319:230.
- Kanai, Y. et. al. (1995) Biochem. Biophys. Res. Commun. 208:1067.
- Codony-Servat, J. et. al. (1999) Cancer Res. 59:1196.
- Carraway, K.L. 3rd et. al. (1994) J. Biol. Chem. 269:14303.
- Emkey, R. and C.R. Kahn (1997) J. Biol. Chem. 272:31172.
- Schaefer, G. et. al. (1999) J. Biol. Chem. 274:859.
- Schlessinger, J. (2000) Cell 103:211.
- Hellyer, N.J. et. al. (2001) J. Biol. Chem. 276:42153.
- Daly, R.J. (1999) Growth Factors 16:255.
Long Name
Receptor Tyrosine Protein Kinase ErbB2
Alternate Names
CD340, HER2, Neu Oncogene, NGL, TKR1
Gene Symbol
ERBB2
UniProt
Additional ErbB2/Her2 Products
Product Documents for Recombinant Human ErbB2/Her2 Fc Chimera Protein, CF
Product Specific Notices for Recombinant Human ErbB2/Her2 Fc Chimera Protein, CF
For research use only
Related Research Areas
Citations for Recombinant Human ErbB2/Her2 Fc Chimera Protein, CF
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Customer Reviews for Recombinant Human ErbB2/Her2 Fc Chimera Protein, CF (10)
4.9 out of 5
10 Customer Ratings
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Application: Binding assay/Protein-protein interactionVerified Customer | Posted 05/20/2021
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Application: Media additive for protein or antibody productionVerified Customer | Posted 02/08/2019
-
Application: Enzymatic activity in vitroVerified Customer | Posted 01/30/2019
-
Application: Apoptosis assayVerified Customer | Posted 12/06/2018
-
Application: Enzymatic activity in vitroVerified Customer | Posted 09/18/2018
-
Application: Characterization prior to use.Verified Customer | Posted 01/10/2018
-
Application: Immunoassay StandardVerified Customer | Posted 12/01/2017
-
Application: Cell ProliferationVerified Customer | Posted 10/20/2017
-
Application: Binding assay/Protein-protein interactionVerified Customer | Posted 05/18/2016
-
Application: ELISA ControlSample Tested: Human recombinant antibodySpecies: HumanVerified Customer | Posted 10/26/2015Used as a control for Human ErbB2/Her2 Antibody (catalog number MAB1129).
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