Recombinant Human FGF-6 Protein

R&D Systems | Catalog # 238-F6

R&D Systems
Discontinued Product
238-F6 has been discontinued. View all FGF-6 products.

Key Product Details

  • R&D Systems E. coli-derived Recombinant Human FGF-6 Protein (238-F6)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

E. coli

Accession Number

P10767.4

Applications

Bioactivity
View all FGF-6 Proteins and Enzymes »

Product Specifications

Source

E. coli-derived human FGF-6 protein
Gly41-Ile208

Purity

>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Gly41 (minor) & Gly67 (major)

Predicted Molecular Mass

19 kDa (Gly41 - Ile208) & 16 kDa (Gly67 - Ile208)

SDS-PAGE

15-22 kDa, reducing conditions

Activity

Measured in a cell proliferation assay using NR6R‑3T3 mouse fibroblast cells. Rizzino, A. et al. (1988) Cancer Res. 48:4266; Thomas, K. et al. (1987) Methods Enzymol. 147:120.
The ED50 for this effect is 0.1-0.3 ng/mL in the presence of 1 µg/mL of heparin.

Formulation, Preparation, and Storage

238-F6
Formulation Lyophilized from a 0.2 μm filtered solution in PBS and CHAPS with BSA as a carrier protein.
Reconstitution Reconstitute at 10 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: FGF-6

Fibroblast Growth Factor-6 (FGF-6), also known as HST-2, is a 25 - 28 kDa member of the FGF family of heparin binding polypeptides which are potent regulators of cell proliferation, differentiation, and function. FGF proteins contain a 120 amino acid (aa) core FGF domain that exhibits a beta -trefoil structure (1, 2). Mature human FGF-6 is a 171 aa protein that shares 94% aa sequence identity with mouse and rat FGF-6 (3). It binds and signals primarily through FGF R1c, 2c, and 4 (4). FGF-6 functions as a mitogen for fibroblasts, vascular endothelial cells, and prostate carcinoma cells, and N-linked glycosylation is required for the full mitogenic effect (5 - 7). FGF-6 expression is restricted to skeletal muscle during development although it can be upregulated in prostate cancer and Kaposi sarcoma (7 - 9). In the adult, FGF-6 is upregulated in injured skeletal muscle and is required for muscle regeneration (10). FGF-6 inhibits the terminal differentiation of myoblasts and also cooperates with TGF-beta 2 to promote chondrogenesis in embryonic somites (8, 11).

References

  1. Wiedlocha, A. and V. Sorensen (2004) Curr. Top. Microbiol. Immunol. 286:45.
  2. Mohammadi, M. et al. (2005) Cytokine Growth Factor Rev. 16:107.
  3. Marics, I. et al. (1989) Oncogene 4:335.
  4. Ornitz, D.M. et al. (1996) J. Biol. Chem. 271:15292.
  5. Pizette, S. et al. (1991) Cell Growth Differ. 2:561.
  6. Asada, M. et al. (1999) Growth Factors 16:293.
  7. Ropiquet, F. et al. (2000) Cancer Res. 60:4245.
  8. de Lapeyriere, O. et al. (1993) Development 118:601.
  9. Li, J. et al. (1993) Cancer 72:2253.
  10. Floss, T. et al. (1997) Genes Dev. 11:2040.
  11. Grass, S. et al. (1996) Development 122:141.

    Long Name

    Fibroblast Growth Factor 6

    Alternate Names

    FGF6, HBGF-6, HST2

    Entrez Gene IDs

    2251 (Human); 14177 (Mouse); 170700 (Rat)

    Gene Symbol

    FGF6

    UniProt

    P10767.4

    Additional FGF-6 Products

    Product Documents for Recombinant Human FGF-6 Protein

    Certificate of Analysis

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    Product Specific Notices for Recombinant Human FGF-6 Protein

    For research use only

    Related Research Areas

    FGF Family
    FGF Ligands

    Citations for Recombinant Human FGF-6 Protein

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