Recombinant Human GLP-1R Fc Chimera Protein, CF

Name: Recombinant Human GLP-1R Fc Chimera Protein, CF
Brand: R&D Systems
SKU: 10646-GL
Rating: 5 (1 reviews)

Catalog #: 10646-GL 1 Review Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
10646-GL-050

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Recombinant Human GLP-1R Fc Chimera Protein Binding Activity.

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All GLP-1R Proteins and Enzymes

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human GLP-1R Fc Chimera Protein, CF Summary

R&D Systems CHO-derived Recombinant Human GLP-1R Fc Chimera Protein (10646-GL)
Quality control testing to verify active proteins with lot specific assays by in-house scientists
All R&D Systems proteins are covered with a 100% guarantee

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. When GLP-1 peptide (Tocris (Catalog # 5374) is immobilized at 0.25 µg/mL (100 µL/well), Recombinant Human GLP-1 Fc Chimera binds with an ED₅₀ of 2-12 ng/mL.

Source

Chinese Hamster Ovary cell line, CHO-derived human GLP-1R protein

Human GLP-1R (Ala21-Glu139) Accession # P43220.2	IEGRMD	Human IgG₁ (Pro100-Lys330)
N-terminus		C-terminus

Accession #

P43220.2

N-terminal Sequence
Analysis

Ala21 & Arg24

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

40 kDa

SDS-PAGE

60-72 kDa, under reducing conditions

Product Datasheets

10646-GL

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10646-GL

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity

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When GLP-1 peptide (5374) is immobilized at 0.25 µg/mL (100 µL/well), Recombinant Human GLP-1R Fc Chimera (Catalog # 10646-GL) binds with an ED₅₀ of 2-12 ng/mL.

SDS-PAGE

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2 μg/lane of Recombinant Human GLP-1R Fc Chimera (Catalog # 10646-GL) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 60-72 kDa and 120-144 kDa, respectively.

Reconstitution Calculator

Background: GLP-1R

Glucagon-like Peptide 1 Receptor (GLP-1R) is a member of the Class B or secretin receptor-like family of G protein-coupled receptors (GPCRs) (1, 2). Activation of GLP-1R signaling regulates secretion of insulin from pancreatic beta -cells in a glucose-dependent manner (3). Human GLP-1R consists of an N-terminal, extracellular domain (ECD) containing 6 conserved cysteine residues and a transmembrane domain with seven membrane-spanning alpha-helices, characteristic of GPCRs (2,4). The mature ECD of human GLP-1R shares 92% amino acid (aa) identity with mouse GLP-1R. GLP-1R is expressed in pancreas, lung, heart, kidney, hypothalamus, and stomach and it is a receptor for Glucagon-like Peptide, a regulator of insulin secretion by pancreatic beta cells (1, 4, 5). GLP1R agonists, including GLP-1, Boc5, and Cpd1, have been targeted for research and used as type 2 diabetes treatments (5, 6). GLP1R is also expressed in the brain where it is involved in the control of appetite (5).

References