Recombinant Human IL-2 GMP Protein, CF

Name: Recombinant Human IL-2 GMP Protein, CF
Brand: R&D Systems
SKU: 202-GMP
Rating: 5 (1 reviews)

Catalog #: 202-GMP
3 Citations 1 Review Datasheet / COA / SDS

Newer Version Available: BT-002-GMP

NEW

Animal-Free

Discontinued Product

202-GMP has been discontinued and is replaced by BT-002-GMP.

Recombinant Human IL-2 GMP Protein Bioactivity

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All IL-2 Proteins and Enzymes

Product Details

Citations (3)

FAQs

Reviews (1)

Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human IL-2 GMP Protein, CF Summary

Product Specifications

Purity

>97%, by SDS-PAGE with silver staining, under reducing conditions. Intact mass analysis of recombinant human IL-2 confirms the predicted molecular mass of 15549 Da

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

Activity

Measured in a cell proliferation assay using CTLL‑2 mouse cytotoxic T cells. Gearing, A.J.H. and C.B. Bird (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 295. The ED₅₀ for this effect is 0.05-0.25 ng/mL.

The specific activity of recombinant human IL-2 is >5.00 x 10⁶ IU/mg, which is calibrated against an internal reference standard value assigned against the human IL-2 WHO International Standard (NIBSC code: 86/500).

Source

E. coli-derived human IL-2 protein
Ala21-Thr153, with an N-terminal Met
Produced using non-animal reagents in an animal-free laboratory.
Manufactured and tested under cGMP guidelines.

Accession #

P60568.1

N-terminal Sequence
Analysis

Met-Ala₂₁-Pro-Thr-Ser-Ser-Ser-Thr-Lys-Lys

Predicted Molecular Mass

15.5 kDa

SDS-PAGE

13 kDa, reducing conditions

Product Datasheets

202-GMP

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

202-GMP

Formulation	Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Reconstitution	Reconstitute at 100 μg/mL in 100 mM acetic acid. Alternatively, reconstitute at 100 μg/mL in sterile deionized water and use within 24 hours, store at 2 to 8 °C.
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. A minimum of 12 months when stored at ≤ -20 °C as supplied. Refer to lot specific COA for the Use by Date. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity

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GMP-grade Recombinant Human IL-2 (Catalog # 202-GMP) stimulates cell proliferation of the CTLL‑2 mouse cytotoxic T cell line. The ED₅₀ for this effect is 0.05-0.25 ng/mL. Three independent lots were tested for activity and plotted on the same graph to show lot-to-lot consistency of GMP IL-2.

Bioactivity

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Equivalent bioactivity of GMP (Catalog # 202-GMP) and Animal-Free (AFL202) grades of Recombinant Human IL-2 as measured in a cell proliferation assay using CTLL 2 mouse cytotoxic T cells (orange and green, respectively).

SDS-PAGE

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1 μg/lane of GMP-grade Recombinant Human IL-2 (Catalog # 202-GMP) was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 13 kDa.

Mass Spectrometry

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MALDI-TOF analysis of GMP-grade Recombinant Human IL-2 (Catalog # 202-GMP). The major peak corresponds to the calculated molecular mass, 15549 Da. The minor peak at 15764 Da is a matrix-associated artifact of the MALDI-TOF.

Reconstitution Calculator

Background: IL-2

Interleukin-2 (IL-2) is a O-glycosylated, four alpha -helix bundle cytokine that has potent stimulatory activity for antigen-activated T cells. It is expressed by CD4⁺ and CD8⁺ T cells, gamma δ T cells, B cells, dendritic cells, and eosinophils (1-3). Mature human IL-2 shares 56% and 66% aa sequence identity with mouse and rat IL-2, respectively. Human and mouse IL-2 exhibit cross-species activity (4). The receptor for IL-2 consists of three subunits that are present on the cell surface in varying preformed complexes (5-7). The 55 kDa IL-2 R alpha is specific for IL-2 and binds with low affinity. The 75 kDa IL-2 R beta, which is also a component of the IL-15 receptor, binds IL-2 with intermediate affinity. The 64 kDa common gamma chain gamma c/IL-2 R gamma, which is shared with the receptors for IL-4, -7, -9, -15, and -21, does not independently interact with IL-2. Upon ligand binding, signal transduction is performed by both IL-2 R beta and gamma c. IL-2 is best known for its autocrine and paracrine activity on T cells. It drives resting T cells to proliferate and induces IL-2 and IL-2 R alpha synthesis (1, 2). It contributes to T cell homeostasis by promoting the Fas-induced death of naïve CD4⁺ T cells but not activated CD4⁺ memory lymphocytes (8). IL-2 plays a central role in the expansion and maintenance of regulatory T cells, although it inhibits the development of Th17 polarized cells (9-11). Thus, IL-2 may be a key cytokine in the natural suppression of autoimmunity (12, 13).

References

Ma, A. et al. (2006) Annu. Rev. Immunol. 24:657.
Gaffen, S.L. and K.D. Liu (2004) Cytokine 28:109.
Taniguchi, T. et al. (1983) Nature 302:305.
Mosmann, T.R. et al. (1987) J. Immunol. 138:1813.
Liparoto, S.F. et al. (2002) Biochemistry 41:2543.
Wang, X. et al. (2005) Science 310:1159.
Bodnar, A. et al. (2008) Immunol. Lett. 116:117.
Jaleco, S. et al. (2003) J. Immunol. 171:61.
Malek, T.R. (2003) J. Leukoc. Biol. 74:961.
Laurence, A. et al. (2007) Immunity 26:371.
Kryczek, I. et al. (2007) J. Immunol. 178:6730.
Afzali, B. et al. (2007) Clin. Exp. Immunol. 148:32.
Fehervari, Z. et al. (2006) Trends Immunol. 27:109.

Long Name

Interleukin 2

Entrez Gene IDs

3558 (Human); 16183 (Mouse); 116562 (Rat); 396868 (Porcine); 280822 (Bovine); 403989 (Canine); 100034204 (Equine); 751114 (Feline); 100302458 (Rabbit)

Alternate Names

Aldesleukin; IL2; IL-2; IL-2lymphokine; interleukin 2; interleukin-2; involved in regulation of T-cell clonal expansion; Proleukin; T cell growth factor; T-cell growth factor; TCGF

Manufacturing Specifications

GMP Proteins
R&D Systems, a Bio-Techne Brand's GMP proteins are produced according to relevant sections of the following documents: USP Chapter 1043, Ancillary Materials for Cell, Gene and Tissue-Engineered Products and Eu. Ph. 5.2.12, Raw Materials of Biological Origin for the Production of Cell-based and Gene Therapy Medicinal Products.

R&D Systems' quality focus includes:

Manufactured and tested under an ISO 9001:2015 and ISO 13485:2016 certified quality system
Documented processes and QA control of documentation and process changes
Personnel training programs
Raw material testing and vendor qualification/monitoring
Fully validated equipment, processes and test methods
Equipment calibration schedules using a computerized calibration program
Facility maintenance, safety programs and pest control
Material review process for variances
Monitoring of stability over product shelf-life

R&D Systems strives to provide our customers with the analytical characteristics of each product so that customers may determine whether our products are appropriate for their research. The Certificate of Analysis provided contains the following lot specific information:

N-terminal amino acid analysis, SDS-PAGE analysis, and endotoxin level (as determined by LAL assay) performed on each bulk QC lot, not on individual bottlings of each QC lot
Post-bottling lot-specific bioassay results (compliance with an established range) and results of microbial testing according to USP
Host Cell Protein testing performed by ELISA
Mycoplasma testing by ribosomal RNA hybridization assay

Additional testing and documentation requested by the customer can be arranged at an additional cost.

Production records and facilities are available for examination by appropriate personnel on-site at R&D Systems in Minneapolis, Minnesota USA.

R&D Systems sells GMP grade products for preclinical or clinical ex vivo use. They are not for in vivo use. Please read the following End User Terms prior to using this product.

Animal-Free Manufacturing Conditions
Our dedicated controlled-access animal-free laboratories ensure that at no point in production are the products exposed to potential contamination by animal components or byproducts. Every stage of manufacturing is conducted in compliance with R&D Systems' stringent Standard Operating Procedures (SOPs). Production and purification procedures use equipment and media that are confirmed animal-free.

Production

All molecular biology procedures use animal-free media and dedicated labware.
Dedicated fermentors are utilized in committed animal-free areas.

Purification

Protein purification columns are animal-free.
Bulk proteins are filtered using animal-free filters.
Purified proteins are stored in animal-free containers in a dedicated cold storage room.

Quality Assurance

Low Endotoxin Level.
No impairment of biological activity.
High quality product obtained under stringent conditions.

Please read our complete Animal-Free Statement.

Product Specific Notices

Full terms and conditions of sale can be found online in the Protein Sciences Segment T&Cs at: Terms & Conditions.

Related Research Areas

Citations for Recombinant Human IL-2 GMP Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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Deciphering regulation of FOXP3 expression in human conventional T cells
Authors: Umhoefer, JM;Arce, MM;Dajani, R;Belk, JA;Mowery, CT;Nguyen, V;Gowen, BG;Simeonov, DR;Curie, GL;Corn, JE;Chang, HY;Marson, A;
bioRxiv : the preprint server for biology
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
Feasibility of iNKT cell and PD-1+CD8+ T cell-based immunotherapy in patients with lung adenocarcinoma: Preliminary results of a phase I/II clinical trial.
Authors: Cheng X, Wang J, Qiu C, Jin Y, Xia B, Qin R, Hu H, Yan J, Zhang X, Xu J
Clin Immunol, 2022-03-30;238(0):108992.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
Eradicating tumor in a recurrent cervical cancer patient with autologous tumor-infiltrating lymphocytes and a modified lymphodepleting regimen.
Authors: Guo J, Luo N, Ai G, Yang W, Zhu J, Li C, Chen R, Zhang C, Liu S, Jin H, Cheng Z
J Immunother Cancer, 2022-02-01;10(2):.
Species: Human
Sample Types: Tissue Homogenates
Applications: Bioassay