Recombinant Human IL-8/CXCL8 Protein

Formulations:
Catalog # Availability Size / Price Qty
208-IL-010
208-IL-050
Recombinant Human IL-8/CXCL8 Protein Data
2 Images
Product Details
Citations (106)
FAQs
Reviews (4)

Recombinant Human IL-8/CXCL8 Protein Summary

Purity
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with human CXCR2. The ED50 for this effect is 0.5-2.5 ng/mL.
Source
E. coli-derived human IL-8/CXCL8 protein
Ser28-Ser99
Accession #
N-terminal Sequence
Analysis
Ser28
Predicted Molecular Mass
8 kDa

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

208-IL

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.

208-IL/CF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Data Images

Bioactivity View Larger

Recombinant Human IL-8/CXCL8 (Catalog # 208-IL) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR2. The ED50for this effect is 0.5-2.5 ng/mL.

SDS-PAGE View Larger

1 μg/lane of Recombinant Human IL-8/CXCL8 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 8 kDa.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-8/CXCL8

Interleukin-8 (IL-8), also known as CXCL8, GCP-1, and NAP-1, is a widely expressed proinflammatory member of the CXC family of chemokines. Near its N-terminus, this 8-9 kDa chemokine contains an ELR motif which is important for its angiogenic properties (1). IL-8/CXCL8 can associate into a homodimer or a heterodimer with CXCL4/PF4 (2), and it can also interact with matrix and cell surface glycosaminoglycans (3). Mature human IL-8/CXCL8 shares 65%-69% amino acid (aa) sequence identiity with canine, feline, and porcine IL-8/CXCL8 (4). There is no IL-8/CXCL8 gene counterpart in rodent. N-terminal truncation by multiple proteases generates a range of shorter forms, and an alternative splice form of human IL-8/CXCL8 carries an eleven aa substitution at the C-terminus (5). The bioactivity of IL-8/CXCL8 is regulated by these truncations, by IL-8/CXCL8 citrullination at Arg5 (N-terminal to the ELR motif) (6), and by the decoy receptor DARC (7). IL-8/CXCL8 effects are mediated through CXCR1/IL-8 RA, which is also used by CXCL6, and through CXCR2/IL-8 RB, which is used by multiple CXC chemokines (1). CXCR1 and CXCR2 associate into functional homodimers and heterodimers with each other (8). Through both CXCR1 and CXCR2, CXCL8 promotes neutrophil adhesion to the vascular endothelium and migration to sites of inflammation (9). It triggers the antimicrobial activation of neutrophils through CXCR1 (10). CXCL8 also binds to Serpin A1/alpha-1 Antitrypsin, and this prevents IL-8/CXCL8 interaction with CXCR1 (11). IL-8/CXCL8 is upregulated in atherosclerotic lesions and other cardiac pathologies where it exacerbates inflammatory tissue damage (12). In addition, it induces VEGF expression, vascular endothelial cell proliferation, angiogenesis, and tumor cell invasiveness (13-16).

References
  1. Lazennec, G. and A. Richmond (2010) Trends Mol. Med. 16:133.
  2. Nesmelova, I.V. et al. (2005) J. Biol. Chem. 280:4948.
  3. Pichert, A. et al. (2012) Biomatter 2:142.
  4. Schmid, J. and C. Weissmann (1987) J. Immunol. 139:250.
  5. Mortier, A. et al. (2008) Pharmacol. Ther. 120:197.
  6. Proost, P. et al. (2008) J. Exp. Med. 205:2085.
  7. Neote, K. et al. (1994) Blood 84:44.
  8. Munoz, L.M. et al. (2009) J. Immunol. 183:7337.
  9. Gerszten, R.E. et al. (1999) Nature 398:718.
  10. Jones, S.A. et al. (1996) Proc. Natl. Acad. Sci. USA 93:6682.
  11. Bergin, D.A. et al. (2010) J. Clin. Invest. 120:4236.
  12. Apostolakis, S. et al. (2009) Cardiovasc. Res. 84:353.
  13. Martin, D. et al. (2009) J. Biol. Chem. 284:6038.
  14. Li, A. et al. (2005) Angiogenesis 8:63.
  15. Waugh, D.J. and C. Wilson (2008) Clin. Cancer Res. 14:6735.
  16. Fernando, R.I. et al. (2011) Cancer Res. 71:5296.
Long Name
Interleukin 8
Entrez Gene IDs
3576 (Human); 396880 (Porcine); 403850 (Canine); 493836 (Feline)
Alternate Names
3-10C; AMCF-I; C-X-C motif chemokine 8; CXCL8; CXCL8SCYB8; Emoctakin; GCP1; GCP-1TSG-1; IL8; IL-8; interleukin 8; K60; LAI; LECT; LUCT; LYNAP; MDNCF; MDNCFb-ENAP; member 8; MONAP; MONAPGCP1; NAF; NAP1; NAP-1NAP1; NCF; Neutrophil-activating protein 1; Protein 3-10C; T cell chemotactic factor; T-cell chemotactic factor; TCF; TSG1

Citations for Recombinant Human IL-8/CXCL8 Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

106 Citations: Showing 1 - 10
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  1. Effective and Rapid Generation of Functional Neutrophils from Induced Pluripotent Stem Cells Using ETV2-Modified mRNA
    Authors: VS Brok-Volch, DA Bennin, K Suknuntha, LC Klemm, A Huttenloch, I Slukvin
    Stem Cell Reports, 2019;13(6):1099-1110.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  2. The Synthetic Dipeptide Pidotimod Shows a Chemokine-Like Activity through CXC Chemokine Receptor 3 (CXCR3)
    Authors: F Caccuri, A Bugatti, S Corbellini, S Roversi, A Zani, P Mazzuca, S Marsico, A Caruso, C Giagulli
    Int J Mol Sci, 2019;20(21):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  3. Effect of iron overload from multi walled carbon nanotubes on neutrophil-like differentiated HL-60 cells
    Authors: Y Tabei, H Fukui, A Nishioka, Y Hagiwara, K Sato, T Yoneda, T Koyama, M Horie
    Sci Rep, 2019;9(1):2224.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  4. A Novel Gastric Spheroid Co-Culture Model Reveals Chemokine-Dependent Recruitment of Human Dendritic Cells to the Gastric Epithelium
    Authors: TA Sebrell, M Hashimi, B Sidar, R Wilkinson, L Kirpotina, MT Quinn, Z Malkoç, PJ Taylor, JN Wilking, D Bimczok
    Cell Mol Gastroenterol Hepatol, 2019;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  5. The BRAF-inhibitor PLX4720 inhibits CXCL8 secretion in BRAFV600E mutated and normal thyroid cells: a further anti-cancer effect of BRAF-inhibitors
    Authors: F Coperchini, L Croce, M Denegri, O Awwad, ST Ngnitejeu, M Muzza, V Capelli, F Latrofa, L Persani, L Chiovato, M Rotondi
    Sci Rep, 2019;9(1):4390.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  6. Secreted nuclear protein DEK regulates hematopoiesis through CXCR2 signaling
    Authors: ML Capitano, N Mor-Vaknin, AK Saha, S Cooper, M Legendre, H Guo, R Contreras-, F Kappes, MA Sartor, CT Lee, X Huang, DM Markovitz, HE Broxmeyer
    J. Clin. Invest., 2019;130(0):2555-2570.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  7. Frontline Science: A flexible kink in the transmembrane domain impairs β2 integrin extension and cell arrest from rolling
    Authors: H Sun, Z Fan, AR Gingras, MA Lopez-Rami, MH Ginsberg, K Ley
    J. Leukoc. Biol., 2019;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  8. MicroRNA-29a Mitigates Subacromial Bursa Fibrosis in Rotator Cuff Lesion with Shoulder Stiffness
    Authors: JY Ko, WS Lian, TC Tsai, YS Chen, CK Hsieh, CW Kuo, FS Wang
    Int J Mol Sci, 2019;20(22):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Culture
  9. Genistein inhibits stemness of SKOV3 cells induced by macrophages co-cultured with ovarian cancer stem-like cells through IL-8/STAT3 axis
    Authors: Y Ning, W Feng, X Cao, K Ren, M Quan, A Chen, C Xu, Y Qiu, J Cao, X Li, X Luo
    J. Exp. Clin. Cancer Res., 2019;38(1):19.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Immunodepletion
  10. HelixComplex snail mucus exhibits pro-survival, proliferative and pro-migration effects on mammalian fibroblasts
    Authors: C Trapella, R Rizzo, S Gallo, A Alogna, D Bortolotti, F Casciano, G Zauli, P Secchiero, R Voltan
    Sci Rep, 2018;8(1):17665.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Bioassay
  11. Neutrophil recruitment to inflamed joints can occur without cellular priming
    Authors: L Björkman, K Christenso, L Davidsson, J Mårtensson, F Amirbeagi, A Welin, H Forsman, A Karlsson, C Dahlgren, J Bylund
    J. Leukoc. Biol., 2018;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  12. Silver nanoparticles promote the emergence of heterogeneic human neutrophil sub-populations
    Authors: JA Fraser, S Kemp, L Young, M Ross, M Prach, GR Hutchison, E Malone
    Sci Rep, 2018;8(1):7506.
    Applications: ELISA (Standard)
  13. Ten weeks of high-intensity interval walk training is associated with reduced disease activity and improved innate immune function in older adults with rheumatoid arthritis: a pilot study
    Authors: DB Bartlett, LH Willis, CA Slentz, A Hoselton, L Kelly, JL Huebner, VB Kraus, J Moss, MJ Muehlbauer, G Spielmann, WE Kraus, JM Lord, KM Huffman
    Arthritis Res. Ther., 2018;20(1):127.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  14. IL-8 associates with a pro-angiogenic and mesenchymal subtype in glioblastoma
    Authors: S Conroy, FAE Kruyt, M Wagemakers, KPL Bhat, WFA den Dunnen
    Oncotarget, 2018;9(21):15721-15731.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  15. Distinct and common expression of receptors for inflammatory mediators in vagal nodose versus jugular capsaicin-sensitive/TRPV1-positive neurons detected by low input RNA sequencing
    Authors: J Wang, M Kollarik, F Ru, H Sun, B McNeil, X Dong, G Stephens, S Korolevich, P Brohawn, R Kolbeck, B Undem
    PLoS ONE, 2017;12(10):e0185985.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  16. C-X-C motif chemokine ligand 8 promotes endothelial cell homing via the Akt-signal transducer and activator of transcription pathway to accelerate healing of ischemic and hypoxic skin ulcers
    Authors: L Shen, P Zhang, S Zhang, L Xie, L Yao, W Lang, J Lian, W Qin, M Zhang, L Ji
    Exp Ther Med, 2017;13(6):3021-3031.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  17. A possible role for neutrophils in allergic rhinitis revealed after cellular subclassification
    Authors: J Arebro, S Ekstedt, E Hjalmarsso, O Winqvist, S Kumlien Ge, LO Cardell
    Sci Rep, 2017;7(0):43568.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  18. Papillary renal cell carcinoma-derived chemerin, IL-8, and CXCL16 promote monocyte recruitment and differentiation into foam-cell macrophages
    Authors: KM Krawczyk, H Nilsson, R Allaoui, D Lindgren, M Arvidsson, K Leandersso, ME Johansson
    Lab. Invest., 2017;97(11):1296-1305.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  19. Possible involvement of NETosis in inflammatory processes in the eye: Evidence from a small cohort of patients
    Authors: T Barliya, R Dardik, Y Nisgav, M Dachbash, D Gaton, G Kenet, R Ehrlich, D Weinberger, T Livnat
    Mol. Vis., 2017;23(0):922-932.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  20. NET-producing CD16(high) CD62L(dim) Neutrophils Migrate to Tumor Sites and Predict Improved Survival in Patients with HNSCC
    Authors: C Rydberg Mi, Å Kågedal, S Kumlien Ge, O Winqvist, R Uddman, R Razavi, E Munck-Wikl, L Olaf Carde
    Int. J. Cancer, 2017;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  21. Cytokines TNF-?, IL-6, IL-17F, and IL-4 Differentially Affect Osteogenic Differentiation of Human Adipose Stem Cells.
    Authors: Angela P Bastidas-C, Astrid D Bakker, Behrouz Zandieh-D, Cornelis J Kleverlaa, Nathalie Bravenboe, Tim Forouzanf, Jenneke Klein-Nul
    Stem Cells International, 2016;0(0):1687-966X.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  22. Macrophages Enhance Migration in Inflammatory Breast Cancer Cells via RhoC GTPase Signaling
    Sci Rep, 2016;6(0):39190.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  23. Interactions between Adipocytes and Breast Cancer Cells Stimulate Cytokine Production and Drive Src/Sox2/miR-302b-Mediated Malignant Progression.
    Authors: Picon-Ruiz M, Pan C, Drews-Elger K, Jang K, Besser A, Zhao D, Morata-Tarifa C, Kim M, Ince T, Azzam D, Wander S, Wang B, Ergonul B, Datar R, Cote R, Howard G, El-Ashry D, Torne-Poyatos P, Marchal J, Slingerland J
    Cancer Res, 2016;76(2):491-504.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  24. Senescent peritoneal mesothelium creates a niche for ovarian cancer metastases
    Cell Death Dis, 2016;7(12):e2565.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  25. Mycobacterium abscessus-Induced Granuloma Formation Is Strictly Dependent on TNF Signaling and Neutrophil Trafficking
    PLoS Pathog., 2016;12(11):e1005986.
    Species: Zebrafish
    Sample Types: In Vivo
    Applications: In Vivo
  26. The Influence of Chronic Wound Extracts on Inflammatory Cytokine and Histatin Stability
    Authors: MA Boink, S Roffel, K Nazmi, C van Montfr, JG Bolscher, A Gefen, EC Veerman, S Gibbs
    PLoS ONE, 2016;11(3):e0152613.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  27. Characterization of the MMP/TIMP Imbalance and Collagen Production Induced by IL-1? or TNF-? Release from Human Hepatic Stellate Cells
    Authors: S Robert, T Gicquel, A Bodin, V Lagente, E Boichot
    PLoS ONE, 2016;11(4):e0153118.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  28. Hyperbaric Oxygen Reduces Production of Reactive Oxygen Species in Neutrophils from Polytraumatized Patients Yielding in the Inhibition of p38 MAP Kinase and Downstream Pathways
    PLoS ONE, 2016;11(8):e0161343.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  29. Adsorption as a Contributor for Inflammatory Mediators Removal by Different Hemofiltration Membranes: A Pilot Study
    Authors: Benjamin Malard
    Artif Organs, 2016;0(0):.
    Species: Human
    Sample Types: Plasma
    Applications: Hemofiltration Procedure
  30. Reciprocal cellular cross-talk within the tumor microenvironment promotes oncolytic virus activity.
    Authors: Ilkow C, Marguerie M, Batenchuk C, Mayer J, Ben Neriah D, Cousineau S, Falls T, Jennings V, Boileau M, Bellamy D, Bastin D, de Souza C, Alkayyal A, Zhang J, Le Boeuf F, Arulanandam R, Stubbert L, Sampath P, Thorne S, Paramanthan P, Chatterjee A, Strieter R, Burdick M, Addison C, Stojdl D, Atkins H, Auer R, Diallo J, Lichty B, Bell J
    Nat Med, 2015;21(5):530-6.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  31. Loss of RACK1 Promotes Metastasis of Gastric Cancer by Inducing a miR-302c/IL8 Signaling Loop.
    Authors: Chen L, Min L, Wang X, Zhao J, Chen H, Qin J, Chen W, Shen Z, Tang Z, Gan Q, Ruan Y, Sun Y, Qin X, Gu J
    Cancer Res, 2015;75(18):3832-41.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  32. Cleavage/alteration of interleukin-8 by matrix metalloproteinase-9 in the female lower genital tract.
    Authors: Zariffard, M Reza, Anastos, Kathryn, French, Audrey L, Munyazesa, Elisapha, Cohen, Mardge, Landay, Alan L, Spear, Gregory
    PLoS ONE, 2015;10(1):e0116911.
    Species: Human
    Sample Types: Vaginal Lavage Fluid
    Applications: Bioassay
  33. Inhibition of T-cell activation by retinal pigment epithelial cells derived from induced pluripotent stem cells.
    Authors: Sugita S, Kamao H, Iwasaki Y, Okamoto S, Hashiguchi T, Iseki K, Hayashi N, Mandai M, Takahashi M
    Invest Ophthalmol Vis Sci, 2015;56(2):1051-62.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  34. The role of phosphoinositide 3-kinases in neutrophil migration in 3D collagen gels.
    Authors: Martin, Kayleigh, Muessel, Michelle, Pullar, Christin, Willars, Gary B, Wardlaw, Andrew J
    PLoS ONE, 2015;10(2):e0116250.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  35. The cerebral cavernous malformation 3 gene is necessary for senescence induction.
    Authors: Guerrero A, Iglesias C, Raguz S, Floridia E, Gil J, Pombo C, Zalvide J
    Aging Cell, 2015;14(2):274-83.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  36. CXCR2 and its related ligands play a novel role in supporting the pluripotency and proliferation of human pluripotent stem cells.
    Authors: Jung J, Lee S, Kim J, Lee S, Sung H, An J, Park Y, Kim B
    Stem Cells Dev, 2015;24(8):948-61.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  37. Chemokine CXCL8 promotes HIV-1 replication in human monocyte-derived macrophages and primary microglia via nuclear factor-kappaB pathway.
    Authors: Mamik M, Ghorpade A
    PLoS ONE, 2014;9(3):e92145.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  38. High activities of BACE1 in brains with mild cognitive impairment.
    Authors: Cheng X, He P, Lee T, Yao H, Li R, Shen Y
    Am J Pathol, 2014;184(1):141-7.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  39. Pulmonary endothelial protein kinase C-delta (PKCdelta) regulates neutrophil migration in acute lung inflammation.
    Authors: Mondrinos M, Zhang T, Sun S, Kennedy P, King D, Wolfson M, Knight L, Scalia R, Kilpatrick L
    Am J Pathol, 2014;184(1):200-13.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  40. Immunogenic calreticulin exposure occurs through a phylogenetically conserved stress pathway involving the chemokine CXCL8.
    Authors: Sukkurwala A, Martins I, Wang Y, Schlemmer F, Ruckenstuhl C, Durchschlag M, Michaud M, Senovilla L, Sistigu A, Ma Y, Vacchelli E, Sulpice E, Gidrol X, Zitvogel L, Madeo F, Galluzzi L, Kepp O, Kroemer G
    Cell Death Differ, 2014;21(1):59-68.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  41. Complex cellular composition of solitary fibrous tumor of the prostate.
    Authors: Gharaee-Kermani M, Mehra R, Robinson D, Wei J, Macoska J
    Am J Pathol, 2014;184(3):732-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  42. Impaired neutrophil extracellular trap formation: a novel defect in the innate immune system of aged individuals.
    Authors: Hazeldine J, Harris P, Chapple I, Grant M, Greenwood H, Livesey A, Sapey E, Lord J
    Aging Cell, 2014;13(4):690-8.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  43. Neutrophil microparticles modulate cytokine production by natural killer cells.
    Authors: Pliyev B, Kalintseva M, Abdulaeva S, Yarygin K, Savchenko V
    Cytokine, 2013;65(2):126-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  44. Human Herpesvirus 8 (HHV8) sequentially shapes the NK cell repertoire during the course of asymptomatic infection and Kaposi sarcoma.
    Authors: Dupuy S, Lambert M, Zucman D, Choukem SP, Tognarelli S, Pages C, Lebbe C, Caillat-Zucman S
    PLoS Pathog., 2012;8(1):e1002486.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  45. Mycobacterium tuberculosis infection and tissue factor expression in macrophages.
    Authors: Kothari H, Rao L, Vankayalapati R, Pendurthi U
    PLoS ONE, 2012;7(9):e45700.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  46. Daily exposure to dust alters innate immunity.
    Authors: Sahlander K, Larsson K, Palmberg L
    PLoS ONE, 2012;7(2):e31646.
  47. Adenovirus-engineered human dendritic cells induce natural killer cell chemotaxis via CXCL8/IL-8 and CXCL10/IP-10.
    Authors: Vujanovic, Lazar, Ballard, Wenners, Thorne, Stephen, Vujanovic, Nikola L, Butterfield, Lisa H
    Oncoimmunology, 2012;1(4):448-457.
  48. Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors.
    Authors: Wilson TR, Fridlyand J, Yan Y, Penuel E, Burton L, Chan E, Peng J, Lin E, Wang Y, Sosman J, Ribas A, Li J, Moffat J, Sutherlin DP, Koeppen H, Merchant M, Neve R, Settleman J
    Nature, 2012;487(7408):505-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  49. Neurexophilin 1 suppresses the proliferation of hematopoietic progenitor cells.
    Authors: Kinzfogl J, Hangoc G, Broxmeyer HE
    Blood, 2011;118(3):565-75.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  50. Delay of migrating leukocytes by the basement membrane deposited by endothelial cells in long-term culture.
    Authors: Burton VJ, Butler LM, McGettrick HM, Stone PC, Jeffery HC, Savage CO, Rainger GE, Nash GB
    Exp. Cell Res., 2011;317(3):276-92.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  51. Phosphoinositide 3-kinase gamma plays a critical role in bleomycin-induced pulmonary inflammation and fibrosis in mice.
    Authors: Russo RC, Garcia CC, Barcelos LS, Rachid MA, Guabiraba R, Roffe E, Souza AL, Sousa LP, Mirolo M, Doni A, Cassali GD, Pinho V, Locati M, Teixeira MM
    J. Leukoc. Biol., 2011;89(2):269-82.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  52. Kallikrein expression and cathelicidin processing are independently controlled in keratinocytes by calcium, vitamin D(3), and retinoic acid.
    Authors: Morizane S, Yamasaki K, Kabigting FD, Gallo RL
    J. Invest. Dermatol., 2010;130(5):1297-306.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  53. IL-8 dictates glycosaminoglycan binding and stability of IL-18 in cystic fibrosis.
    Authors: Reeves EP, Williamson M, Byrne B, Bergin DA, Smith SG, Greally P, O'Kennedy R, O'Neill SJ, McElvaney NG
    J. Immunol., 2010;184(3):1642-52.
    Species: Human
    Sample Types: Carbohydrates
    Applications: Binding Assay
  54. CCL19 is a specific ligand of the constitutively recycling atypical human chemokine receptor CRAM-B.
    Authors: Leick M, Catusse J, Follo M, Nibbs RJ, Hartmann TN, Veelken H, Burger M
    Immunology, 2010;129(4):536-46.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  55. RAGE and ICAM-1 cooperate in mediating leukocyte recruitment during acute inflammation in vivo.
    Authors: Frommhold D, Kamphues A, Hepper I, Pruenster M, Lukic IK, Socher I, Zablotskaya V, Buschmann K, Lange-Sperandio B, Schymeinsky J, Ryschich E, Poeschl J, Kupatt C, Nawroth PP, Moser M, Walzog B, Bierhaus A, Sperandio M
    Blood, 2010;116(5):841-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  56. Oxidized alpha1-antitrypsin stimulates the release of monocyte chemotactic protein-1 from lung epithelial cells: potential role in emphysema.
    Authors: Li Z, Alam S, Wang J, Sandstrom CS, Janciauskiene S, Mahadeva R
    Am. J. Physiol. Lung Cell Mol. Physiol., 2009;297(2):L388-400.
    Species: N/A
    Sample Types: N/A
    Applications: ELISA (Standard)
  57. IL-17 induces monocyte migration in rheumatoid arthritis.
    Authors: Shahrara S, Pickens SR, Dorfleutner A, Pope RM
    J. Immunol., 2009;182(6):3884-91.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  58. Chemerin expression marks early psoriatic skin lesions and correlates with plasmacytoid dendritic cell recruitment.
    Authors: Albanesi C, Scarponi C, Pallotta S, Daniele R, Bosisio D, Madonna S, Fortugno P, Gonzalvo-Feo S, Franssen JD, Parmentier M, De Pita O, Girolomoni G, Sozzani S
    J. Exp. Med., 2009;206(1):249-58.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  59. Interleukin-7 stimulates secretion of S100A4 by activating the JAK/STAT signaling pathway in human articular chondrocytes.
    Authors: Yammani RR, Long D, Loeser RF
    Arthritis Rheum., 2009;60(3):792-800.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  60. Estradiol increases IL-8 secretion of normal human breast tissue and breast cancer in vivo.
    Authors: Bendrik C, Dabrosin C
    J. Immunol., 2009;182(1):371-8.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  61. Prostate cancer promotes CD11b positive cells to differentiate into osteoclasts.
    Authors: Mizutani K, Sud S, Pienta KJ
    J. Cell. Biochem., 2009;106(4):563-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  62. Spatiotemporal expression of chemokines and chemokine receptors in experimental anti-myeloperoxidase antibody-mediated glomerulonephritis.
    Authors: van der Veen BS, Petersen AH, Belperio JA, Satchell SC, Mathieson PW, Molema G, Heeringa P
    Clin. Exp. Immunol., 2009;158(1):143-53.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  63. Maternal and cord plasma cytokine and chemokine profile in pregnancies complicated by asthma.
    Authors: Osei-Kumah A, Smith R, Clifton VL
    Cytokine, 2008;43(2):187-93.
    Species: N/A
    Sample Types: N/A
    Applications: ELISA (Standard)
  64. Regulation of D6 chemokine scavenging activity by ligand- and Rab11-dependent surface up-regulation.
    Authors: Bonecchi R, Borroni EM, Anselmo A, Doni A, Savino B, Mirolo M, Fabbri M, Jala VR, Haribabu B, Mantovani A, Locati M
    Blood, 2008;112(3):493-503.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  65. Cognate CD4+ T-cell-dendritic cell interactions induce migration of immature dendritic cells through dissolution of their podosomes.
    Authors: Nobile C, Lind M, Miro F, Chemin K, Tourret M, Occhipinti G, Dogniaux S, Amigorena S, Hivroz C
    Blood, 2008;111(7):3579-90.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  66. Cytokines induced during chronic hepatitis B virus infection promote a pathway for NK cell-mediated liver damage.
    Authors: Dunn C, Brunetto M, Reynolds G, Christophides T, Kennedy PT, Lampertico P, Das A, Lopes AR, Borrow P, Williams K, Humphreys E, Afford S, Adams DH, Bertoletti A, Maini MK
    J. Exp. Med., 2007;204(3):667-80.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  67. Growth factor induction of Cripto-1 shedding by glycosylphosphatidylinositol-phospholipase D and enhancement of endothelial cell migration.
    Authors: Watanabe K, Bianco C, Strizzi L, Hamada S, Mancino M, Bailly V, Mo W, Wen D, Miatkowski K, Gonzales M, Sanicola M, Seno M, Salomon DS
    J. Biol. Chem., 2007;282(43):31643-55.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  68. Ultrasensitive flow-based immunoassays using single-molecule counting.
    Authors: Todd J, Freese B, Lu A, Held D, Morey J, Livingston R, Goix P
    Clin. Chem., 2007;53(11):1990-5.
    Species: N/A
    Sample Types: N/A
    Applications: ELISA (Standard)
  69. Tumour necrosis factor-alpha (TNF-alpha) increases nuclear factor kappaB (NFkappaB) activity in and interleukin-8 (IL-8) release from bovine mammary epithelial cells.
    Authors: Fitzgerald DC, Meade KG, McEvoy AN, Lillis L, Murphy EP, Machugh DE, Baird AW
    Vet. Immunol. Immunopathol., 2007;116(1):59-68.
    Species: N/A
    Sample Types: N/A
    Applications: ELISA (Standard)
  70. S100A11, an dual mediator for growth regulation of human keratinocytes.
    Authors: Sakaguchi M, Sonegawa H, Murata H, Kitazoe M, Futami J, Kataoka K, Yamada H, Huh NH
    Mol. Biol. Cell, 2007;19(1):78-85.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  71. Fluorescence single-molecule counting assays for high-sensitivity detection of cytokines and chemokines.
    Authors: Qui H, Ferrell EP, Nolan N, Phelps BH, Tabibiazar R, Whitney DH, Naelfski EA
    Clin. Chem., 2007;53(11):2010-2.
    Species: N/A
    Sample Types: N/A
    Applications: ELISA (Standard)
  72. Murine CXCR1 is a functional receptor for GCP-2/CXCL6 and interleukin-8/CXCL8.
    Authors: Fan X, Patera AC, Pong-Kennedy A, Deno G, Gonsiorek W, Manfra DJ, Vassileva G, Zeng M, Jackson C, Sullivan L, Sharif-Rodriguez W, Opdenakker G, Van Damme J, Hedrick JA, Lundell D, Lira SA, Hipkin RW
    J. Biol. Chem., 2007;282(16):11658-66.
    Species: Mouse
    Sample Types: Complex Sample Type
    Applications: Binding Assay
  73. TF/FVIIa transactivate PDGFRbeta to regulate PDGF-BB-induced chemotaxis in different cell types: involvement of Src and PLC.
    Authors: Siegbahn A, Johnell M, Nordin A, Aberg M, Velling T
    Arterioscler. Thromb. Vasc. Biol., 2007;28(1):135-41.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  74. IL-1beta-stimulated activation of ERK1/2 and p38alpha MAPK mediates the transcriptional up-regulation of IL-6, IL-8 and GRO-alpha in HeLa cells.
    Authors: Yang HT, Cohen P, Rousseau S
    Cell. Signal., 2007;20(2):375-80.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  75. Increased expression of Th2-associated chemokines in bullous pemphigoid disease. Role of eosinophils in the production and release of these chemokines.
    Authors: Gounni Abdelilah S, Wellemans V, Agouli M, Guenounou M, Hamid Q, Beck LA, Lamkhioued B
    Clin. Immunol., 2006;120(2):220-31.
  76. Apoptotic neutrophils release macrophage migration inhibitory factor upon stimulation with tumor necrosis factor-alpha.
    Authors: Daryadel A, Grifone RF, Simon HU, Yousefi S
    J. Biol. Chem., 2006;281(37):27653-61.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  77. Proteinase-activated receptor2 agonists upregulate granulocyte colony-stimulating factor, IL-8, and VCAM-1 expression in human bronchial fibroblasts.
    Authors: Ramachandran R, Morice AH, Compton SJ
    Am. J. Respir. Cell Mol. Biol., 2006;35(1):133-41.
    Species: N/A
    Sample Types: N/A
    Applications: ELISA (Standard)
  78. PAPP-A and osteoprotegerin, together with interleukin-8 and RANTES, are elevated in the peritoneal fluid of women with endometriosis.
    Authors: Bersinger NA, von Roten S, Wunder DM, Raio L, Dreher E, Mueller MD
    Am. J. Obstet. Gynecol., 2006;195(1):103-8.
    Species: N/A
    Sample Types: N/A
    Applications: ELISA (Standard)
  79. Increased levels of neutrophil-activating peptide-2 in acute coronary syndromes: possible role of platelet-mediated vascular inflammation.
    Authors: Smith C, Damas JK, Otterdal K, ØIe E, Sandberg WJ, Yndestad A, Waehre T, Scholz H, Endresen K, Olofsson PS, Halvorsen B, Gullestad L, Froland SS, Hansson GK, Aukrust P
    J. Am. Coll. Cardiol., 2006;48(8):1591-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  80. Novel antiviral activity of chemokines.
    Authors: Nakayama T, Shirane J, Hieshima K, Shibano M, Watanabe M, Jin Z, Nagakubo D, Saito T, Shimomura Y, Yoshie O
    Virology, 2006;350(2):484-92.
    Species: Virus
    Sample Types: Virus
    Applications: Binding Assay
  81. Combination therapy: Synergistic suppression of virus-induced chemokines in airway epithelial cells.
    Authors: Edwards MR, Johnson MW, Johnston SL
    Am. J. Respir. Cell Mol. Biol., 2006;34(5):616-24.
    Species: N/A
    Sample Types: N/A
    Applications: ELISA (Standard)
  82. Lack of in vitro and in vivo recognition of Francisella tularensis subspecies lipopolysaccharide by Toll-like receptors.
    Authors: Hajjar AM, Harvey MD, Shaffer SA, Goodlett DR, Sjostedt A, Edebro H, Forsman M, Bystrom M, Pelletier M, Wilson CB, Miller SI, Skerrett SJ, Ernst RK
    Infect. Immun., 2006;74(12):6730-8.
    Species: N/A
    Sample Types: N/A
    Applications: ELISA (Standard)
  83. The injury response of oligodendrocyte precursor cells is induced by platelets, macrophages and inflammation-associated cytokines.
    Authors: Rhodes KE, Raivich G, Fawcett JW
    Neuroscience, 2006;140(1):87-100.
    Species: Rat
    Sample Types: In Vivo
    Applications: In Vivo
  84. An inflammation-induced mechanism for leukocyte transmigration across lymphatic vessel endothelium.
    Authors: Johnson LA, Clasper S, Holt AP, Lalor PF, Baban D, Jackson DG
    J. Exp. Med., 2006;203(12):2763-77.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  85. Transforming growth factor-alpha: a major human serum factor that promotes human keratinocyte migration.
    Authors: Li Y, Fan J, Chen M, Li W, Woodley DT
    J. Invest. Dermatol., 2006;126(9):2096-105.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  86. Increased ENA-78 in the follicular fluid of patients with endometriosis.
    Authors: Wunder DM, Mueller MD, Birkhauser MH, Bersinger NA
    Acta Obstet Gynecol Scand, 2006;85(3):336-42.
    Species: N/A
    Sample Types: N/A
    Applications: ELISA (Standard)
  87. Proinflammatory mediators elicit secretion of the intracellular B-lymphocyte stimulator pool (BLyS) that is stored in activated neutrophils: implications for inflammatory diseases.
    Authors: Scapini P, Carletto A, Nardelli B, Calzetti F, Roschke V, Merigo F, Tamassia N, Pieropan S, Biasi D, Sbarbati A, Sozzani S, Bambara L, Cassatella MA
    Blood, 2005;105(2):830-7.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  88. Expression of PDGF and their receptors in human retinal pigment epithelial cells and fibroblasts: regulation by TGF-beta.
    Authors: Nagineni CN, Kutty V, Detrick B, Hooks JJ
    J. Cell. Physiol., 2005;203(1):35-43.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  89. Expression and activity of SRC regulate interleukin-8 expression in pancreatic adenocarcinoma cells: implications for angiogenesis.
    Authors: Trevino JG, Summy JM, Gray MJ, Nilsson MB, Lesslie DP, Baker CH, Gallick GE
    Cancer Res., 2005;65(16):7214-22.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  90. Interleukin-6 and interleukin-8 in cervical fluid in a population of Swedish women in preterm labor: relationship to microbial invasion of the amniotic fluid, intra-amniotic inflammation, and preterm delivery.
    Authors: Holst RM, Mattsby-Baltzer I, Wennerholm UB, Hagberg H, Jacobsson B
    Acta Obstet Gynecol Scand, 2005;84(6):551-7.
    Species: N/A
    Sample Types: N/A
    Applications: ELISA (Standard)
  91. Tissue inhibitor of metalloproteinase-1 deficiency amplifies acute lung injury in bleomycin-exposed mice.
    Authors: Kim KH, Burkhart K, Chen P, Frevert CW, Randolph-Habecker J, Hackman RC, Soloway PD, Madtes DK
    Am. J. Respir. Cell Mol. Biol., 2005;33(3):271-9.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  92. Human CXCR2 (hCXCR2) takes over functionalities of its murine homolog in hCXCR2 knockin mice.
    Authors: Mihara K, Smit MJ, Krajnc-Franken M, Gossen J, Rooseboom M, Dokter W
    Eur. J. Immunol., 2005;35(9):2573-82.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  93. Specific CXC but not CC chemokines cause elevated monocyte migration in COPD: a role for CXCR2.
    Authors: Traves SL, Smith SJ, Barnes PJ, Donnelly LE
    J. Leukoc. Biol., 2004;76(2):441-50.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  94. IL-17 markedly up-regulates beta-defensin-2 expression in human airway epithelium via JAK and NF-kappaB signaling pathways.
    Authors: Kao CY, Chen Y, Thai P, Wachi S, Huang F, Kim C, Harper RW, Wu R
    J. Immunol., 2004;173(5):3482-91.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  95. Plasmacytoid dendritic cell recruitment by immobilized CXCR3 ligands.
    Authors: Kohrgruber N, Groger M, Meraner P, Kriehuber E, Petzelbauer P, Brandt S, Stingl G, Rot A, Maurer D
    J. Immunol., 2004;173(11):6592-602.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  96. Direct comparison of traditional ELISAs and membrane protein arrays for detection and quantification of human cytokines.
    Authors: Copeland S, Siddiqui J, Remick D
    J. Immunol. Methods, 2004;284(1):99-106.
    Species: N/A
    Sample Types: N/A
    Applications: ELISA (Standard)
  97. 17beta-estradiol (E2) modulates cytokine and chemokine expression in human monocyte-derived dendritic cells.
    Authors: Bengtsson AK, Ryan EJ, Giordano D, Magaletti DM, Clark EA
    Blood, 2004;104(5):1404-10.
    Species: N/A
    Sample Types: N/A
    Applications: ELISA (Standard)
  98. Rapid analysis of inflammatory cytokines in cerebrospinal fluid using chip-based immunoaffinity electrophoresis.
    Authors: Phillips TM
    Electrophoresis, 2004;25(10):1652-9.
    Species: N/A
    Sample Types: N/A
    Applications: Standard
  99. Evasion from NK cell immunity by MHC class I chain-related molecules expressing colon adenocarcinoma.
    Authors: Doubrovina ES, Doubrovin MM, Vider E, Sisson RB, O&amp;amp;apos;Reilly RJ, Dupont B, Vyas YM
    J. Immunol., 2003;171(12):6891-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  100. Expression of functional CCR and CXCR chemokine receptors in podocytes.
    Authors: Huber TB, Reinhardt HC, Exner M, Burger JA, Kerjaschki D, Saleem MA, Pavenstadt H
    J. Immunol., 2002;168(12):6244-52.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  101. Distribution, cellular localization and functional role of CCR2 chemokine receptors in adult rat brain.
    Authors: Banisadr G, Queraud-Lesaux F, Boutterin MC, Pelaprat D, Zalc B, Rostene W, Haour F, Parsadaniantz SM
    J. Neurochem., 2002;81(2):257-69.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: Binding Assay
  102. Imbalance in the expression of CXC chemokines correlates with bronchoalveolar lavage fluid angiogenic activity and procollagen levels in acute respiratory distress syndrome.
    Authors: Keane MP, Donnelly SC, Belperio JA, Goodman RB, Dy M, Burdick MD, Fishbein MC, Strieter RM
    J. Immunol., 2002;169(11):6515-21.
    Species: N/A
    Sample Types: N/A
    Applications: ELISA (Standard)
  103. Secretion of oncostatin M by infiltrating neutrophils: regulation of IL-6 and chemokine expression in human mesothelial cells.
    Authors: Hurst SM, McLoughlin RM, Monslow J, Owens S, Morgan L, Fuller GM, Topley N, Jones SA
    J. Immunol., 2002;169(9):5244-51.
    Species: N/A
    Sample Types: N/A
    Applications: ELISA (Standard)
  104. Metabolic replacement of kidney function in uremic animals with a bioartificial kidney containing human cells.
    Authors: Humes HD, Fissell WH, Weitzel WF, Buffington DA, Westover AJ, MacKay SM, Gutierrez JM
    Am. J. Kidney Dis., 2002;39(5):1078-87.
    Species: N/A
    Sample Types: N/A
    Applications: ELISA (Standard)
  105. Chemoattractant factors in breast milk from allergic and nonallergic mothers.
    Authors: Bottcher MF, Jenmalm MC, Bjorksten B, Garofalo RP
    Pediatr. Res., 2000;47(5):592-7.
    Species: N/A
    Sample Types: N/A
    Applications: ELISA (Standard)
  106. Complement fragment C5a and inflammatory cytokines in neutrophil recruitment during intramammary infection with Escherichia coli.
    Authors: Shuster DE, Kehrli ME, Rainard P, Paape M
    Infect. Immun., 1997;65(8):3286-92.
    Species: Bovine
    Sample Types: Milk
    Applications: Bioassay

FAQs

  1. Are mouse or rat CXCL8/IL-8 products offered?

    • Mouse and rat do not have a gene which encodes CXCL8/IL-8. In mouse, the functional homologs to human CXCL8/IL-8 are CXCL1/KC and CXCL2/MIP-2. In rat, the functional homolog to CXCL8/human IL-8 is CXCL3/CINC-2.

  2. What is the difference between 208-IL (rhCXCL8/IL-8) and  618-IL (rhEndothelial Cell-Derived IL-8/CXCL8)?

    • Several N-terminal processed forms of CXCL8/IL-8 are produced by proteolytic cleavage after secretion by cells such as peripheral blood monocytes, leukcocytes and endothelial cells. Catalog # 208-IL consists only of the sequence Ser28 to Ser99 (also known as IL-8, 6-77), which is the most prominent form of IL-8. According to literature, IL-8(6-77) has a 5-10-fold higher activity in neutrophil activation. Catalog # 618-IL consists of Ser28 to Ser99 (IL-8, 6-77) and Ala23 to Ser99 (IL-8).  In our chemoattraction assay, 208-IL has a 2-fold higher activity than 618-IL.Several N-terminal processed forms of CXCL8/IL-8 are produced by proteolytic cleavage after secretion by cells such as peripheral blood monocytes, leukcocytes and endothelial cells. Catalog # 208-IL consists only of the sequence Ser28 to Ser99 (also known as IL-8, 6-77), which is the most prominent form of IL-8. According to literature, IL-8(6-77) has a 5-10-fold higher activity in neutrophil activation. Catalog # 618-IL consists of Ser28 to Ser99 (IL-8, 6-77) and Ala23 to Ser99 (IL-8).  In our chemoattraction assay, 208-IL has a 2-fold higher activity than 618-IL.

  3. Can Recombinant Human IL-8/CXCL8 Protein (Catalog # 208-IL) be used with mouse cells?

    • Bioactivity of Recombinant Human IL-8/CXCL8 Protein (Catalog # 208-IL) has only been evaluated using human cells transfected with human CXCR2 (the human receptor for IL-8), and we have not evaluated the activity of this protein using mouse cells. There is no IL-8/CXCL8 gene counterpart in rodent species. We are aware of a few publications citing use of 208-IL in mouse assay systems, so we would recommend reviewing the publications listed on the Citations tab of the product-specific web page to find reported use of our products in similar experimental layouts.

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Reviews for Recombinant Human IL-8/CXCL8 Protein

Average Rating: 4.8 (Based on 4 Reviews)

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Recombinant Human IL-8/CXCL8 Protein
By Anonymous on 12/03/2018
Application: In vitro bioactivity in cell culture

rhIL-8 induce β-arrestin release in CXCR1/U2OS reporter cell line


Recombinant Human IL-8/CXCL8 Protein
By Anonymous on 11/26/2018
Application: Immunoassay Standard

The recombinant human IL-8 protein was used as the calibration reagent in MSD assay with biotinylated MAB208 (capture) and Sulfo-Tag labeled AF-208-NA (detection) antibodies.


Recombinant Human IL-8/CXCL8 Protein
By Anonymous on 11/10/2017
Application: activation of neutrophils from blood in vitro.
Reason for Rating: It worked well for the activation of neutrophils.

Recombinant Human IL-8/CXCL8 Protein
By Anonymous on 04/07/2017
Application: Neutrophils were activated with 208-IL and assessed for morphological changes and migratory activity on 2D cell culture plate.