Recombinant Human/Mouse/Rat GDF-8/Myostatin Protein, CF New

Catalog #: 11658-G8 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
11658-G8-020
11658-G8-050
11658-G8-100
11658-G8-250

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Recombinant Human/Mouse/Rat GDF‑8/Myostatin Protein Bioactivity.

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human/Mouse/Rat GDF-8/Myostatin Protein, CF Summary

R&D Systems CHO-derived Recombinant Human/Mouse/Rat GDF-8/Myostatin Protein (11658-G8)
Quality control testing to verify active proteins with lot specific assays by in-house scientists
All R&D Systems proteins are covered with a 100% guarantee

Product Specifications

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its ability to induce cytotoxicity using MPC-11 mouse B lymphocyte cells. The ED₅₀ for this effect is 4.00-40.0 ng/mL.

Source

Chinese Hamster Ovary cell line, CHO-derived GDF-8/Myostatin protein
Asp267-Ser375

Accession #

O14793.1

N-terminal Sequence
Analysis

Asp267

Structure / Form

Disulfide linked homodimer

Predicted Molecular Mass

12 kDa

SDS-PAGE

10-14 kDa, under reducing conditions.

Product Datasheets

11658-G8

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

11658-G8

Formulation	Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with Trehalose.
Reconstitution	Reconstitute at 250 μg/mL in water.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity

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Recombinant Human/Mouse/Rat GDF-8/Myostatin Protein (Catalog # 11658-G8) induces cytotoxicity on MCP-11 cells. The ED₅₀ for this effect is 4.00-40.0 ng/mL.

SDS-PAGE

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1 μg/lane of Recombinant Human/Mouse/Rat GDF‑8/Myostatin Protein (Catalog # 11658-G8) was resolved with SDS-PAGE under reducing (R) condition and visualized by silver staining, showing bands at 10-14 kDa.

Reconstitution Calculator

Background: GDF-8/Myostatin

Growth Differentiation Factor 8 (GDF-8), also known as myostatin, is a member of the TGF-beta superfamily that is expressed specifically in developing and adult skeletal muscle. GDF-8 cDNA encodes a 376 amino acid (aa) prepropeptide with a 24 aa residue signal peptide, a 223 aa residue amino-terminal propeptide, and a 109 aa residue carboxy-terminal mature protein. Mature GDF-8 contains the canonical 7-cysteine motif common to other TGF-beta superfamily members. Similar to the TGF‑ beta s, activins and BMP-11, GDF-8 also contains one extra pair of cysteine residues that is not found in other family members. The bioactive form of GDF-8 is a homodimer with an apparent molecular weight of approximately 25 kDa. GDF-8 is highly conserved across species. At the amino acid sequence level, mature human, mouse, rat and cow GDF-8 are 100% identical. Within the TGF-beta superfamily, GDF-8 is most closely related to BMP-11, a mammalian protein that acts as a dorsal mesoderm and neural inducer in Xenopus explants. The two proteins share 90% amino acid sequence identity within their mature chain. A targeted disruption of GDF-8 in mouse results in large mice with a widespread increase in skeletal muscle mass, indicating that GDF-8 is a negative regulator of skeletal muscle growth. A mutation in the bovine GDF-8 gene has been shown to be responsible for the double-muscled phenotype in cattle breeds such as Belgian Blue cattle that is characterized by an increase in muscle mass. GDF-8 has also been shown to inhibit preadipocyte differentiation to adipocytes. Mature GDF-8 binds to activin type II receptors and the binding is antagonized by the activin-binding protein, follistatin. R&D Systems recombinant GDF-8 preparations have been shown to act similarly to Activin A in both the Xenopus animal cap and the K562 assays.

References