Recombinant Human PRDC/GREM2 Protein, CF

Catalog #: 8436-PR Datasheet / COA / SDS

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8436-PR-050

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Recombinant Human PRDC/GREM2 Protein Bioactivity

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human PRDC/GREM2 Protein, CF Summary

R&D Systems E. coli-derived Recombinant Human PRDC/GREM2 Protein (8436-PR)
Quality control testing to verify active proteins with lot specific assays by in-house scientists
All R&D Systems proteins are covered with a 100% guarantee

Product Specifications

Purity

>95%, by SDS-PAGE with silver staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its ability to inhibit BMP-4-induced activity in MC3T3‑E1 mouse preosteoblast cells. The ED₅₀ for this effect is 0.03-0.12 μg/mL

Source

E. coli-derived human PRDC/GREM2 protein
Arg22-Gln168 with an N-terminal Met

Accession #

Q9H772

N-terminal Sequence
Analysis

Met

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

17 kDa

SDS-PAGE

18 kDa, reducing conditions

Product Datasheets

8436-PR

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

8436-PR

Formulation	Lyophilized from a 0.2 μm filtered solution in HCl.
Reconstitution	Reconstitute at 500 μg/mL in 4 mM HCl
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity

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Recombinant Human PRDC/GREM2 (Catalog # 8436-PR) inhibits BMP-4-induced activity in MC3T3‑E1 mouse preosteoblast cells. The ED₅₀ for this effect is 0.03-0.12 μg/mL

Reconstitution Calculator

Background: PRDC/GREM2

PRDC/GREM2 (Protein Related to DAN and Cerberus/Gremlin-2) is a secreted BMP antagonist belonging to the Cerberus/DAN (CAN) family. Similar to other CAN family members, PRDC/GREM2 has 6 conserved cysteine residues that form a cysteine knot, and two additional cysteine residues located in the loops of the cysteine knot (1-3). Mature human PRDC/GREM2 is synthesized as a 168 amino acid (aa) monomer. At the amino acid level, mature human PRDC/GREM2 shares 97% sequence identity with mature mouse PRDC/GREM2. PRDC/GREM2 is biologically active as a homodimer that assembles its subunits in a head-to-tail orientation. PRDC/GREM2 is up-regulated through Wnt/ beta -catenin signaling and has been shown to potently inhibit BMP-2, -4, and -7 (4-6). During early development PRDC/GREM2 is involved in tooth morphogenesis, osteogenesis, and neurogenesis (7-9). In the adult, PRDC/GREM2 is involved in bone repair and bone remodeling (10). PRDC/GREM2 has been detected in the adult ovary, brain, spleen, and colon (11). The biological activity of PRDC/GREM2 can be blocked by binding to heparin (4).

References

Pearce, J.J. et al. (1999) Dev. Biol. 209:98.
Kattamuri, C. et al. (2012) Protein Expr. Purif. 82:389.
Minabe-Saegusa, C. et al. (1998) Dev. Growth Differ. 40:343.
Nolan, K. et al. (2013) Structure 21:1417.
Kattamuri, C. et al. (2012) J. Mol. Biol. 424:313.
Im, J. et al. (2007) Biochem. Biophys. Res. Commun. 354:296.
Vogel, P. et al. (2014) Vet. Pathol. [Epub ahead of print; PMID 24686385].
Kriebitz, N.N. et al. (2009) Dev. Biol. 336:280.
Ideno, H. et al. (2009) Exp. Cell Res. 315:474.
Dean, D.B. et al. (2010) J. Anat. 216:625.
Sudo, S. et al. (2004) J. Biol. Chem. 279:23134.

Long Name

Protein Related to DAN and Cerberus/Gremlin-2

Entrez Gene IDs

64388 (Human); 23893 (Mouse)

Alternate Names

CKTSF1B2; CKTSF1B2FLJ21195; Cysteine knot superfamily 1, BMP antagonist 2; DAN domain family member 3; DAND3; DAND3Prdc; GREM2; gremlin 2; gremlin 2, cysteine knot superfamily, homolog; gremlin-2; PRDC; PRDCgremlin 2, cysteine knot superfamily, homolog (Xenopus laevis); Protein related to DAN and cerberus