Recombinant Human PSGL-1/CD162 Fc Chimera Protein, CF

Catalog # Availability Size / Price Qty
3345-PS-050
Recombinant Human PSGL-1/CD162 Fc Chimera Protein Bioactivity
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Product Details
Citations (13)
FAQs
Supplemental Products
Reviews (1)

Recombinant Human PSGL-1/CD162 Fc Chimera Protein, CF Summary

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by the ability of the immobilized protein to support the adhesion of CHO Chinese hamster ovary cells transfected with P-Selectin. The ED50 for this effect is 0.04-0.32 µg/mL.
Source
Chinese Hamster Ovary cell line, CHO-derived human PSGL-1/CD162 protein
Human PSGL-1
(Gln42-Val295)
Accession # AAC50061
IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus
Accession #
N-terminal Sequence
Analysis

Gln42

Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
52.6 kDa (monomer)
SDS-PAGE
100-150 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

3345-PS

Formulation Lyophilized from a 0.2 μm filtered solution in Tris and NaCl.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Image

Bioactivity Recombinant Human PSGL-1/CD162 Fc Chimera Protein Bioactivity View Larger

Recombinant Human PSGL-1/CD162 Fc Chimera (Catalog # 3345-PS) supports the adhesion of CHO Chinese hamster ovary cells transfected with P-Selectin. The ED50for this effect is 0.04-0.32 μg/mL.

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Background: PSGL-1/CD162

PSGL-1 (P-Selectin Glycoprotein Ligand-1, designated CD162), is a 110-120 kDa mucin-type glycoprotein that plays a key role in leukocyte adhesion (1-3). Human PSGL-1 cDNA encodes 412 amino acids (aa) including a 17 aa signal sequence, a 24 aa propeptide, a 279 aa extracellular domain (ECD), a 21 aa transmembrane segment and a 71 aa cytoplasmic region (1). The ECD of human PSGL-1 contains 16 decameric repeats in the A allele (long form), which is expressed by 65-80% of the population. Alleles B and C contain 15 and 14 repeats, respectively, and mouse PSGL-1 contains 10-12 repeats (1, 3). Shorter forms may show weaker binding to P-selectin (9). Human PSGL-1 ECD shares less than 50% aa sequence identity with mouse, equine, canine and porcine PSGL-1 ECD. However, all share structural motifs and functions (1-3). The mature PSGL-1 (aa 42-412) is expressed as a disulfide-linked homodimer that signals intracellularly and promotes integrin activation (2‑4). Ligand binding relies on presence of O-glycosylation/sialylated Lewis X (LeX) and tyrosine sulfation within aa 42-57 (1, 5-7). Sulfation and glycosylation, which are constitutive on neutrophils and induced with activation on T cells, are essential for binding to P-selectin and L-selectin (3-6). E-Selectin requires only glycosylation (3). Proteases such as neutrophil elastase and cathepsin G can cleave within this region, impacting binding affinity (3, 8). Cleaved, soluble ectodomains of PSGL-1 are also known (3). PSGL-1 is found on virtually all leukocytes, dendritic cells, platelets, and some endothelial cells (3). It is primarily responsible for early events in extravasation, especially rolling adhesion of leukocytes to vascular endothelium (3, 4). In addition, PGSL-1 binds chemokines such as CCL19, CCL21 and CCL27, promoting chemotaxis of hematopoietic stem cells and plasma cells to the bone marrow and T cell homing to lymphoid organs (3, 10, 11). Recombinant PSGL-1 ECD interferes with this chemotaxis (13). PSGL-1 binds von Willebrand factor in endothelial-derived Weibel-Palade bodies, and the chondroitin sulfate, versican (3). It is present with Tissue Factor in pro-thrombotic microparticles from membranes of monocytes and cancer cells, and is required for particle shedding and fusion with activated platelets (12, 13).

References
  1. Yang, J. et al. (1999) Thromb. Haemost. 81:1.
  2. Sako, D. et al. (1993) Cell 75:1179.
  3. Carlow, D.A. et al. (2009) Immunol. Rev. 230:75.
  4. Zarbock, A. et al. (2011) Blood 278:37.
  5. Bernimoulin, M.P. et al. (2003) J. Biol. Chem. 278:37.
  6. Sako, D. et al. (1995) Cell 83:323.
  7. Xia, L. et al. (2003) Blood 101:552.
  8. Gardiner, E.E. et al. (2001) Blood 98:1440.
  9. Lozano, M.L. et al. (2001) Br. J. Haematol. 115:969.
  10. Veerman, K.M. et al. (2007) Nat. Immunol. 8:532.
  11. Hirata, T. et al. (2004) J. Biol. Chem. 279:51775.
  12. del Conde, I. et al. (2005) Blood 106:1604.
  13. Thomas, G.M. et al. (2009) J Exp. Med. 206:1913.
Long Name
P-Selectin Glycoprotein Ligand 1
Entrez Gene IDs
6404 (Human); 20345 (Mouse); 363930 (Rat)
Alternate Names
CD162 antigen; CD162; P-selectin glycoprotein ligand 1; PSGL1; PSGL-1; PSGL-1cutaneous lymphocyte-associated associated antigen; selectin P ligandCLA; SELPLG

Citations for Recombinant Human PSGL-1/CD162 Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

13 Citations: Showing 1 - 10
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  1. The mucin-selective protease StcE enables molecular and functional analysis of human cancer-associated mucins
    Authors: SA Malaker, K Pedram, MJ Ferracane, BA Bensing, V Krishnan, C Pett, J Yu, EC Woods, JR Kramer, U Westerlind, O Dorigo, CR Bertozzi
    Proc. Natl. Acad. Sci. U.S.A., 2019;0(0):.
    Species: Bacteria - E. Coli
    Sample Types: Recombinant Protein
    Applications: Bioassay
  2. Recombinant Human Vimentin Binds to P-Selectin and Blocks Neutrophil Capture and Rolling on Platelets and Endothelium
    Authors: FW Lam, Q Da, B Guillory, MA Cruz
    J. Immunol., 2018;0(0):.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Blocking
  3. Synthesis of Human Neutrophil Extracellular Traps Contributes to Angiopoietin-Mediated In Vitro Proinflammatory and Proangiogenic Activities
    Authors: SS Lavoie, E Dumas, B Vulesevic, PE Neagoe, M White, MG Sirois
    J. Immunol., 2018;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  4. Soluble Siglec-5 associates to PSGL-1 and displays anti-inflammatory activity
    Sci Rep, 2016;6(0):37953.
    Species: N/A
    Sample Types: Recombinant Protein
    Applications: Bioassay
  5. TleA, a Tsh-like autotransporter identified in a human enterotoxigenic Escherichia coli strain.
    Authors: Gutierrez D, Pardo M, Montero D, Onate A, Farfan M, Ruiz-Perez F, Del Canto F, Vidal R
    Infect Immun, 2015;83(5):1893-903.
    Species: Bacteria - E. Coli
    Sample Types: Cell Culture Supernates
    Applications: Bioassay
  6. Single Neutralizing Monoclonal Antibodies Targeting the VP1 GH Loop of Enterovirus 71 Inhibit both Virus Attachment and Internalization during Viral Entry.
    Authors: Ku Z, Ye X, Shi J, Wang X, Liu Q, Huang Z
    J Virol, 2015;89(23):12084-95.
    Species: Human
    Sample Types: Complex Sample Type
    Applications: Bioassay
  7. Broad spectrum activity of a lectin-like bacterial serine protease family on human leukocytes.
    Authors: Ayala-Lujan, Jorge Lu, Vijayakumar, Vidhya, Gong, Mei, Smith, Rachel, Santiago, Araceli, Ruiz-Perez, Fernando
    PLoS ONE, 2014;9(9):e107920.
    Species: Bacteria
    Sample Types: Protein
    Applications: Enzyme Assay Substrate
  8. Heterotropic modulation of selectin affinity by allosteric antibodies affects leukocyte rolling.
    Authors: Riese S, Kuehne C, Tedder T, Hallmann R, Hohenester E, Buscher K
    J Immunol, 2014;192(4):1862-9.
    Species: Human
    Sample Types: Protein
    Applications: Bioassay
  9. Bromelain decreases neutrophil interactions with P-selectin, but not E-selectin, in vitro by proteolytic cleavage of P-selectin glycoprotein ligand-1.
    Authors: Banks, Jessica, Herman, Christin, Bailey, Ryan C
    PLoS ONE, 2013;8(11):e78988.
    Species: N/A
    Sample Types: Recombinant Protein
    Applications: Bioassay
  10. Regulation of leukocyte recruitment by the long pentraxin PTX3.
    Authors: Deban L, Russo RC, Sironi M, Moalli F, Scanziani M, Zambelli V, Cuccovillo I, Bastone A, Gobbi M, Valentino S, Doni A, Garlanda C, Danese S, Salvatori G, Sassano M, Evangelista V, Rossi B, Zenaro E, Constantin G, Laudanna C, Bottazzi B, Mantovani A
    Nat. Immunol., 2010;11(4):328-34.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Binding Assay
  11. A versatile polyacrylamide gel electrophoresis based sulfotransferase assay.
    Authors: Wu ZL, Ethen CM, Larson S, Prather B, Jiang W
    BMC Biotechnol., 2010;10(0):11.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Enzyme Assay
  12. Kinetic analysis of heparin and glucan sulfates binding to P-selectin and its impact on the general understanding of selectin inhibition.
    Authors: Simonis D, Fritzsche J, Alban S, Bendas G
    Biochemistry, 2007;46(20):6156-64.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Surface Plasmon Resonance
  13. Crystal structures of the staphylococcal toxin SSL5 in complex with sialyl Lewis X reveal a conserved binding site that shares common features with viral and bacterial sialic acid binding proteins.
    Authors: Baker HM, Basu I, Chung MC, Caradoc-Davies T, Fraser JD, Baker EN
    J. Mol. Biol., 2007;374(5):1298-308.
    Species: N/A
    Sample Types: N/A
    Applications: Western Blot

FAQs

  1. Does the CHO-derived  rhPSGL-1/CD162, Catalog # 3345-PS, have O-glycosylation and the Sialyl-LewisX modification?

    • Yes. We use proprietary methods to produce a functional rhPSGL-1 protein with O-glycosylation and the Sialyl-Lewis X Modification. 

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Recombinant Human PSGL-1/CD162 Fc Chimera Protein, CF
By Anonymous on 08/07/2019
Application: Binding assay/Protein-protein interaction
Reason for Rating: Good binding for protein-protein interaction assays