Recombinant Human Tsukushi/TSK Protein, CF

Catalog #: 3940-TS
1 Citation Datasheet / COA / SDS

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3940-TS-050

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Recombinant Human Tsukushi/TSK Protein, CF Summary

R&D Systems NS0-derived Recombinant Human Tsukushi/TSK Protein (3940-TS)
Quality control testing to verify active proteins with lot specific assays by in-house scientists
All R&D Systems proteins are covered with a 100% guarantee

Product Specifications

Purity

>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. Immobilized rhBMP-4 at 1 µg/mL can bind rhTSK with an apparent K_D <15 nM.

Source

Mouse myeloma cell line, NS0-derived human Tsukushi/TSK protein
Thr17-Leu353, with a C-terminal 6-His tag

Accession #

Q8WUA8

N-terminal Sequence
Analysis

Thr17

Predicted Molecular Mass

40 kDa

SDS-PAGE

40 kDa, reducing conditions

Product Datasheets

3940-TS

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

3940-TS

Formulation	Lyophilized from a 0.2 μm filtered solution in HCl.
Reconstitution	Reconstitute at 100 μg/mL in sterile 4 mM HCl.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: Tsukushi/TSK

TSK, also known as Tsukushi, is an atypical member of the small leucine-rich proteoglycan (SLRP) family of extracellular matrix proteins (1, 2). All contain leucine-rich repeats (LRR) flanked by conserved cysteines, but the C-terminus of TSK resembles the structure of nyctalopin and chondroadherin rather than class I - III SLRPs (1). The human TSK cDNA encodes a 16 amino acid (aa) signal sequence and a 337 aa secreted (presumably glycosylated) protein that contains nine LRR (2). Proteoglycan modification of TSK has not been shown. SLRP-like activity or expression of TSK has been identified in several contexts. TSK mRNA is upregulated by estrogen treatment in breast cancer cell lines, and so is proposed to be involved in hormonally regulated extracellular matrix remodeling (2). TSK is upregulated along with bone markers in Vitamin K2-treated osteosarcoma cell lines, mediated by the pregnane X receptor, PXR (3). TSK was also shown to contribute to vitamin K2-mediated enhancement of collagen accumulation. Other SLRPs have been shown to form a protective coat around collagen fibrils, shielding them from proteolysis (4). In rats, expression of TSK in liver, intestines and islets of Langerhans resembles the pattern of Glut2 (5). TSK transcription is upregulated by both estrogen and insulin in hepatocytes. In Xenopus and chick embryos, TSK is expressed in the middle primitive streak, where it binds and antagonizes BMP-4 and promotes dorsalization and formation of the neural crest and the primitive organizer (6, 7). Xenopus TSK also binds the extracellular region of delta-1, modulating Notch activity (7). Human TSK shows 87%, 86%, 86%, 85%, 52% and 50% aa identity with canine, mouse, rat, bovine, chick, and Xenopus TSK, respectively, with the highest identity in the first four LRR.

References

McEwan, P.A. et al. (2006) J. Struct. Biol. 155:294.
Charpentier, A.H. et al. (2000) Cancer Res. 60:5977.
Ichikawa, T. et al. 2006, J. Biol. Chem. 281:16927.
Geng, Y. et al. (2006) Matrix Biol. 25:484.
Coffy, S. et al. (2005) Biochem. J. 385:165.
Ohta, K. et al. (2004) Dev. Cell 7:347.
Kuriyama, S. et al. (2006) Development 133:75.

Long Name

Tsukushin Leucine-rich Repeat Containing Protein 54

Entrez Gene IDs

25987 (Human)

Alternate Names

E2IG4; E2IG4E2-induced gene 4 protein; leucine rich repeat containing 54; Leucine-rich repeat-containing protein 54; LRRC54; TSK; TSKtsukushi homolog; TSKU; tsukushi small leucine rich proteoglycan homolog (Xenopus laevis); Tsukushi; tsukushi, small leucine rich proteoglycan; tsukushin

Related Research Areas

Citation for Recombinant Human Tsukushi/TSK Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

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