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Recombinant Human UCH-L1/PGP9.5 Protein, CF

Catalog #: E-340
5 Citations 1 Review Datasheet / COA / SDS

Discontinued Product

E-340 has been discontinued.
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Recombinant Human UCH-L1/PGP9.5 Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.
Activity
Recombinant Human UCH-L1 is a Ubiquitin-specific deconjugating enzyme. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human UCH-L1 concentration of 10-50 nM. A 15 minute pre-incubation with 10 mM DTT is recommended to achieve maximum activity.
Source
E. coli-derived human UCH-L1/PGP9.5 protein
Accession #
P09936
Predicted Molecular Mass

25 kDa

Product Datasheets

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E-340

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

E-340

Formulation X mg/ml (X μM) in 50 mM HEPES pH 8.0, 100 mM NaCl, 1 mM EDTA, 2 mM DTT
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: UCH-L1/PGP9.5

Ubiquitin Carboxyl-terminal Esterase L1 (UCH-L1), also known as PGP 9.5, is a deubiquitinating enzyme with a predicted molecular weight of 25 kDa (1). The human protein shares 95% amino acid sequence identity with its mouse and rat orthologs. UCH-L1 is expressed abundantly in neurons, accounting for 1-2% of total soluble proteins in the brain (2). It localizes primarily to the cytoplasm, but a subpopulation has been shown to be transiently nuclear (3,4). UCH-L1 contains two catalytic residues, Cys90 and His161, which are required for isopeptide bond cleavage at the C-terminal glycine residue of Ubiquitin (5). The levels of free Ubiquitin appear to be partially regulated by UCH-L1 through the hydrolysis of small Ubiquitin chains and the stabilization of monomeric Ubiquitin (6). Mice lacking functional UCH-L1 show neuronal dysfunction and neurodegeneration, and mutations in this enzyme have been linked to Parkinson's disease, suggesting that it is important for proper central nervous system function (7,8). UCH-L1 also likely plays a complex role in cancer. It has been reported to function as an oncogene in lymphoma, colorectal cancer, and nonsmall cell lung carcinoma (9-11). In contrast, it is thought to function as a tumor suppressor protein in nasopharyngeal and breast cancers (12,13).

References
  1. Wilkinson, K.D. et al. (1989) Science 246:670.
  2. Solano, S.M. et al. (2000) Ann. Neurol. 47:201.
  3. Henderson, M.J. et al. (2010) J. Biol. Chem. 285:11314.
  4. Bheda, A. et al. (2010) Cell Cycle 9:980.
  5. Larsen, C.N. et al. (1996) Biochemistry 35:6735.
  6. Walters, B.J. et al. (2008) Mol. Cell. Neurosci. 39:539.
  7. Sakurai, M. et al. (2008) Eur. J. Neurosci. 27:691.
  8. Andersson, F.I. et al. (2011) J. Mol. Biol. 407:261.
  9. Hussain, S. et al. (2010) Leukemia 24:1641.
  10. Akishima-Fukasawa, Y. et al. (2010) Am. J. Clin. Pathol. 134:71.
  11. Kim, H.J. et al. (2009) Oncogene 28:117.
  12. Li, L. et al. (2010) Clin. Cancer Res. 16:2949.
  13. Xiang, T. et al. (2012) PLoS One 7:e29783.
 
Long Name
Ubiquitin C-terminal Hydrolase L1
Entrez Gene IDs
7345 (Human); 22223 (Mouse)
Alternate Names
EC 3.4.19.12; EC 6.-; Neuron cytoplasmic protein 9.5; PARK5; PGP 9.5; PGP9.5; PGP9.5Uch-L1; PGP95; ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase); ubiquitin carboxyl-terminal hydrolase isozyme L1; ubiquitin C-terminal hydrolase; Ubiquitin thioesterase L1; UCHL1; UCH-L1

Citations for Recombinant Human UCH-L1/PGP9.5 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. Reactive-site-centric chemoproteomics identifies a distinct class of deubiquitinase enzymes
    Authors: DS Hewings, J Heideker, TP Ma, AP AhYoung, F El Oualid, A Amore, GT Costakes, D Kirchhofer, B Brasher, T Pillow, N Popovych, T Maurer, C Schwerdtfe, WF Forrest, K Yu, J Flygare, M Bogyo, IE Wertz
    Nat Commun, 2018-03-21;9(1):1162.
    Species: Human
    Sample Types: Protein
    Applications: Bioassay
  2. Overexpression of ubiquitin carboxyl-terminal hydrolase 1 (UCHL1) in boys with cryptorchidism
    Authors: D Toliczenko, E Matuszczak, M Tylicka, B Szyma?ska, M Komarowska, E Gorodkiewi, W Debek, A Hermanowic
    PLoS ONE, 2018-02-05;13(2):e0191806.
    Species: Human
    Sample Types: Complex Sample Type
    Applications: Bioassay
  3. Overexpression of ubiquitin carboxyl-terminal hydrolase L1 (UCHL1) in serum of children after thermal injury
    Authors: E Matuszczak, M Tylicka, W D?bek, A Sankiewicz, E Gorodkiewi, A Hermanowic
    Adv Med Sci, 2017-02-11;62(1):83-86.
    Applications: Bioassay
  4. Proteasome addiction defined in Ewing's sarcoma is effectively targeted by a novel class of 19S proteasome inhibitors
    Authors: Neerav N Shukla
    Cancer Res, 2016-06-02;0(0):.
    Species: Human
    Sample Types: Protein
    Applications: Bioassay
  5. A selective USP1-UAF1 inhibitor links deubiquitination to DNA damage responses.
    Authors: Liang, Qin, Dexheimer, Thomas S, Zhang, Ping, Rosenthal, Andrew S, Villamil, Mark A, You, Changjun, Zhang, Qiuting, Chen, Junjun, Ott, Christin, Sun, Hongmao, Luci, Diane K, Yuan, Bifeng, Simeonov, Anton, Jadhav, Ajit, Xiao, Hui, Wang, Yinsheng, Maloney, David J, Zhuang, Zhihao
    Nat Chem Biol, 2014-02-16;10(4):298-304.
    Applications: Bioassay

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Recombinant Human UCH-L1/PGP9.5 Protein, CF
By Anonymous on 03/18/2019
Application: Enzymatic activity in vitro
Enzymatic activity in vitro Recombinant Human UCH-L1/PGP9.5 Protein, CF E-340