Recombinant Mouse Chordin Protein

Carrier Free

Catalog # Availability Size / Price Qty
758-CN-050/CF

With Carrier

Catalog # Availability Size / Price Qty
758-CN-050
R&D Systems Recombinant Proteins and Enzymes
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Product Details
Citations (11)
FAQs
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Recombinant Mouse Chordin Protein Summary

Product Specifications

Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to inhibit BMP-4-induced alkaline phosphatase production by ATDC5 mouse chondrogenic cells. The ED50 for this effect is 2‑6 µg/mL in the presence of 30 ng/mL of Recombinant Human BMP-4 (Catalog # 314-BP) and 1 µg/mL of recombinant mouse TSG.
Source
Mouse myeloma cell line, NS0-derived mouse Chordin protein
Mouse Chordin
(Thr27-Ser948)
Accession # Q9Z0E2
IEGR GGGSGGGSGGGS 10-His tag
N-terminus C-terminus
Accession #
N-terminal Sequence
Analysis
Thr27
Predicted Molecular Mass
101.5 kDa
SDS-PAGE
100-115 kDa, reducing conditions

Product Datasheets

758-CN (with carrier)

758-CN/CF (carrier free)

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

758-CN

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 200 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

758-CN/CF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 200 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Chordin

Chordin is a secreted glycoprotein that regulates dorsoventral patterning during gastrulation. Chordin functions as a bone morphogenetic protein (BMP) antagonist that blocks their ventralizing activity by binding to the BMPs and inhibiting their interaction with their receptors. Mouse Chordin cDNA encodes a 948 amino acid (aa) residue precursor protein with a putative 26 aa residue signal peptide. Chordin contains four internal cysteine-rich repeats (CRs) that are conserved in the spacing of their ten cysteine residues. The CRs of chordin, especially CR1 and CR3, have been shown to be the functional domains for BMP binding. These conserved CRs are present in an expanding family of secreted molecules that antagonize BMP signaling. Xolloid (an extracellular zinc metalloproteinase) can cleave chordin at two specific sites resulting in chordin fragments with lower BMP-affinity. Cleavage of the chordin/BMP complex can reverse the BMP antagonist activity of chordin. Mouse chordin is expressed at high levels in 7 day postcoitum mouse embryos. Chordin expression is also detected in multiple fetal and adult tissues, most notably liver and cerebellum, suggesting additional roles for chordin in organogenesis and homeostasis.

References
  1. De Robertis, E.M. and Y. Sasai (1996) Nature 380:37.
  2. Larrain, J. et al. (2000) Development 127:821.
  3. Coffinier, C. et al. (2001) Mech. Dev. 100:119.
Entrez Gene IDs
8646 (Human); 12667 (Mouse)
Alternate Names
Chordin; CHRD; MGC133038; Sog

Citations for Recombinant Mouse Chordin Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

11 Citations: Showing 1 - 10
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  1. Hippocampal overexpression of chordin protects against the chronic social defeat stress-induced depressive-like effects in mice
    Authors: CN Wang, SN Gong, W Guan, JL Wang, TT Gao, Y Wang, F Sun, B Jiang
    Brain Res. Bull., 2020;158(0):31-39.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  2. Functional and structural studies of tolloid-like 1 (TLL1) mutants associated with atrial-septal defect 6 (ASD6)
    Authors: L Sieron, M Lesiak, I Schisler, Z Drzazga, A Fertala, AL Sieron
    Biosci. Rep., 2018;0(0):.
    Species: Mouse
    Sample Types: Protein
    Applications: Bioassay
  3. Chordin-induced lineage plasticity of adult SVZ neuroblasts after demyelination.
    Authors: Jablonska B, Aguirre A, Raymond M, Szabo G, Kitabatake Y, Sailor KA, Ming GL, Song H, Gallo V
    Nat. Neurosci., 2010;13(5):541-50.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  4. Crossveinless-2 is required for the relocalization of Chordin protein within the vertebral field in mouse embryos.
    Authors: Zakin L, Chang EY, Plouhinec JL, De Robertis EM
    Dev. Biol., 2010;347(1):204-15.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  5. A concentration-dependent endocytic trap and sink mechanism converts Bmper from an activator to an inhibitor of Bmp signaling.
    Authors: Kelley R, Ren R, Pi X, Wu Y, Moreno I, Willis M, Moser M, Ross M, Podkowa M, Attisano L, Patterson C
    J. Cell Biol., 2009;184(4):597-609.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  6. Self-renewing epiblast stem cells exhibit continual delineation of germ cells with epigenetic reprogramming in vitro.
    Authors: Hayashi K, Surani MA
    Development, 2009;136(21):3549-56.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  7. Cross-talk between fibroblast growth factor and bone morphogenetic proteins regulates gap junction-mediated intercellular communication in lens cells.
    Authors: Boswell BA, Lein PJ, Musil LS
    Mol. Biol. Cell, 2008;19(6):2631-41.
    Species: Chicken
    Sample Types: Whole Tissue
    Applications: Bioassay
  8. Crossveinless-2 Is a BMP feedback inhibitor that binds Chordin/BMP to regulate Xenopus embryonic patterning.
    Authors: Ambrosio AL, Taelman VF, Lee HX, Metzinger CA, Coffinier C, De Robertis EM
    Dev. Cell, 2008;15(2):248-60.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Binding Assay
  9. Activin a efficiently specifies definitive endoderm from human embryonic stem cells only when phosphatidylinositol 3-kinase signaling is suppressed.
    Authors: McLean AB, D&amp;amp;apos;Amour KA, Jones KL, Krishnamoorthy M, Kulik MJ, Reynolds DM, Sheppard AM, Liu H, Xu Y, Baetge EE, Dalton S
    Stem Cells, 2007;25(1):29-38.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  10. Activin A is an endogenous inhibitor of ureteric bud outgrowth from the Wolffian duct.
    Authors: Maeshima A, Vaughn DA, Choi Y, Nigam SK
    Dev. Biol., 2006;295(2):473-85.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: Bioassay
  11. Regulation of ADMP and BMP2/4/7 at opposite embryonic poles generates a self-regulating morphogenetic field.
    Authors: Reversade B, De Robertis EM
    Cell, 2005;123(6):1147-60.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Receptor Binding Assay

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