SDF-1 alpha and SDF-1 beta, members of the chemokine alpha subfamily that lack the ELR domain, were initially identified using the signal sequence trap cloning strategy from a mouse bone-marrow stromal cell line. These proteins were subsequently also cloned from a human stromal cell line as cytokines that supported the proliferation of a stromal cell‑dependent pre-B-cell line, and were named Pre‑B‑Cell Growth Stimulating Factor (PBSF). SDF‑1 alpha and SDF‑1 beta cDNAs encode precursor proteins of 89 and 93 amino acid residues, respectively. Both SDF-1 alpha and SDF‑1 beta are encoded by a single gene and arise by alternative splicing. The two proteins are identical except for the four amino acid residues that are present in the carboxy‑terminus of SDF-1 beta and absent from SDF‑1 alpha. SDF‑1/PBSF is highly conserved between species, with only one amino acid substitution between the mature human and mouse proteins. SDF‑1/PBSF acts via the chemokine receptor CXCR4 and has been shown to be a chemoattractant for T‑lymphocytes, monocytes, pro- and pre- B cells, but not neutrophils. Mice lacking SDF-1 or CXCR4 have been found to have impaired B‑lymphopoiesis, myelopoiesis, vascular development, cardiogenesis and abnormal neuronal cell migration and patterning in the central nervous system.
Recombinant Mouse CXCL12/SDF-1 alpha Protein
R&D Systems | Catalog # 460-SD
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Key Product Details
- R&D Systems E. coli-derived Recombinant Mouse CXCL12/SDF-1 alpha Protein (460-SD)
- Quality control testing to verify active proteins with lot specific assays by in-house scientists
- All R&D Systems proteins are covered with a 100% guarantee
Source
E. coli
Accession Number
Applications
Bioactivity
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Product Specifications
Source
E. coli-derived mouse CXCL12/SDF-1 alpha protein
Lys22-Lys89
Lys22-Lys89
Purity
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Lys22
Predicted Molecular Mass
8 kDa
Activity
Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with human CXCR4.
The ED50 for this effect is 0.0300-0.360 ng/mL.
The ED50 for this effect is 0.0300-0.360 ng/mL.
Reviewed Applications
Read 10 reviews rated 5 using 460-SD in the following applications:
Scientific Data Images for Recombinant Mouse CXCL12/SDF-1 alpha Protein
Recombinant Mouse CXCL12/SDF-1 alpha Protein Bioactivity
Recombinant Mouse CXCL12/SDF-1 alpha (Catalog # 460-SD) chemoattracts the BaF3 mouse pro-B cells transfected with human CXCR4. The ED50 for this effect is 0.0300-0.360 ng/mL.
Recombinant Mouse CXCL12/SDF-1 alpha Protein SDS-PAGE
1 μg/lane of Recombinant Mouse CXCL12/SDF-1 alpha was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 7 kDa.Formulation, Preparation, and Storage
Carrier Free
What does CF mean?CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
What formulation is right for me?In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
Carrier: 460-SD
| Formulation | Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Carrier Free: 460-SD/CF
| Formulation | Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Calculators
Background: CXCL12/SDF-1 alpha
References
- Ma, Q. et al. (1998) Proc. Natl. Acad. Sci. USA 95:9448.
- Zou, Y.R. et al. (1998) Nature 393:595.
- Tachibana, K. et al. (1998) Nature 393:591.
Alternate Names
SDF1 alpha, SDF1a
Gene Symbol
CXCL12
UniProt
Additional CXCL12/SDF-1 alpha Products
Product Documents for Recombinant Mouse CXCL12/SDF-1 alpha Protein
Product Specific Notices for Recombinant Mouse CXCL12/SDF-1 alpha Protein
For research use only
Related Research Areas
Citations for Recombinant Mouse CXCL12/SDF-1 alpha Protein
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Customer Reviews for Recombinant Mouse CXCL12/SDF-1 alpha Protein (10)
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Application: In vitro bioactivity in cell cultureVerified Customer | Posted 11/17/2021
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Application: In vitro bioactivity in cell cultureVerified Customer | Posted 04/23/2021
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Application: In vitro bioactivity in cell cultureVerified Customer | Posted 01/29/2021
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Application: Characterization prior to use in downstream assay.Verified Customer | Posted 11/04/2019
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Application: SDS-PAGE ControlVerified Customer | Posted 04/15/2019
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Application: Cell migration/motilityVerified Customer | Posted 01/28/2019
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Application: Enzymatic activity in vitroVerified Customer | Posted 01/28/2019
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Application: Cell migration/motilityVerified Customer | Posted 12/16/2016
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Application: Cell migration/motilityVerified Customer | Posted 08/03/2016
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Application: In vitro bioactivity in cell cultureVerified Customer | Posted 05/17/2016
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