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Recombinant Mouse IL-2 Protein

R&D Systems | Catalog # 402-ML

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Key Product Details

  • R&D Systems E. coli-derived Recombinant Mouse IL-2 Protein (402-ML)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

E. coli

Accession Number

P04351

Applications

Bioactivity
View all IL-2 Proteins and Enzymes »

Product Specifications

Source

E. coli-derived mouse IL-2 protein
Ala21-Gln169, with and without an N-terminal Met

Purity

>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala21 & Met

Predicted Molecular Mass

17.2 kDa

Activity

Measured in a cell proliferation assay using CTLL‑2 mouse cytotoxic T cells. Gearing, A.J.H. and C.B. Bird (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 295.
The ED50 for this effect is 0.1-0.4 ng/mL.

Reviewed Applications

Read 9 reviews rated 4.8 using 402-ML in the following applications:

Scientific Data Images for Recombinant Mouse IL-2 Protein

Recombinant Mouse IL-2 Protein Bioactivity

Recombinant Mouse IL-2 Protein Bioactivity

Recombinant Mouse IL-2 (Catalog # 402-ML) stimulates cell proliferation of the CTLL-2 mouse cytotoxic T cell line. The ED50 for this effect is 0.1-0.4 ng/mL.
Recombinant Mouse IL-2 Protein SDS-PAGE

Recombinant Mouse IL-2 Protein SDS-PAGE

1 μg/lane of Recombinant Mouse IL-2 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a band(s) at 19 kDa.

Formulation, Preparation, and Storage

Carrier Free
What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Carrier: 402-ML
Formulation Lyophilized from a 0.2 μm filtered solution in Sodium Citrate and Tween®-80 with BSA as a carrier protein.
Reconstitution Reconstitute at 100-200 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Carrier Free: 402-ML/CF
Formulation Lyophilized from a 0.2 μm filtered solution in Sodium Citrate and Tween®-80.
Reconstitution Reconstitute at 100-200 μg/mL in sterile deionized water.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-2

Interleukin‑2 (IL‑2) is a O‑glycosylated four alpha ‑helix bundle cytokine that has potent stimulatory activity for antigen‑activated T cells. It is expressed by CD4+ and CD8+ T cells, gamma delta T cells, B cells, dendritic cells, and eosinophils (1‑3). Mature mouse IL‑2 shares 56% and 73% aa sequence identity with human and rat IL‑2, respectively. It shows strain‑specific heterogeneity in an N‑terminal region that contains a poly‑glutamine stretch (4). Mouse and human IL‑2 exhibit cross‑species activity (5). The receptor for IL‑2 consists of three subunits that are present on the cell surface in varying preformed complexes (6‑8). The 55 kDa IL‑2 R alpha is specific for IL‑2 and binds with low affinity. The 75 kDa IL‑2 R beta, which is also a component of the IL‑15 receptor, binds IL‑2 with intermediate affinity. The 64 kDa common gamma chain gamma c/IL-2 R gamma, which is shared with the receptors for IL‑4, ‑7, ‑9, ‑15, and ‑21, does not independently interact with IL‑2. Upon ligand binding, signal transduction is performed by both IL‑2 R beta and gamma c. IL‑2 is best known for its autocrine and paracrine activity on T cells. It drives resting T cells to proliferate and induces IL‑2 and IL‑2 R alpha synthesis (1, 2). It contributes to T cell homeostasis by promoting the Fas‑induced death of naïve CD4+ T cells but not activated CD4+ memory lymphocytes (9). IL‑2 plays a central role in the expansion and maintenance of regulatory T cells, although it inhibits the development of Th17 polarized cells (10‑12). Thus, IL‑2 may be a key cytokine in the natural suppression of autoimmunity (13, 14).

References

  1. Ma, A. et al. (2006) Annu. Rev. Immunol. 24:657.
  2. Gaffen, S.L. and K.D. Liu (2004) Cytokine 28:109.
  3. Kashima, N. et al. (1985) Nature 313:402.
  4. Matesanz, F. et al. (1993) Immunogenetics 38:300.
  5. Mosmann, T.R. et al. (1987) J. Immunol. 138:1813.
  6. Liparoto, S.F. et al. (2002) Biochemistry 41:2543.
  7. Wang, X. et al. (2005) Science 310:1159.
  8. Bodnar, A. et al. (2008) Immunol. Lett. 116:117.
  9. Jaleco, S. et al. (2003) J. Immunol. 171:61.
  10. Malek, T.R. (2003) J. Leukoc. Biol. 74:961.
  11. Laurence, A. et al. (2007) Immunity 26:371.
  12. Kryczek, I. et al. (2007) J. Immunol. 178:6730.
  13. Afzali, B. et al. (2007) Clin. Exp. Immunol. 148:32.
  14. Fehervari, Z. et al. (2006) Trends Immunol. 27:109.

Long Name

Interleukin 2

Alternate Names

Aldesleukin, IL2, Proleukin, TCGF

Entrez Gene IDs

3558 (Human); 16183 (Mouse); 116562 (Rat); 396868 (Porcine); 280822 (Bovine); 403989 (Canine); 100034204 (Equine); 751114 (Feline); 100302458 (Rabbit)

Gene Symbol

IL2

UniProt

P04351

Additional IL-2 Products

Product Documents for Recombinant Mouse IL-2 Protein

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS 402-ML (with carrier)
Download SDS 402-ML/CF (carrier free)

Product Specific Notices for Recombinant Mouse IL-2 Protein

For research use only

Citations for Recombinant Mouse IL-2 Protein

Customer Reviews for Recombinant Mouse IL-2 Protein (9)

4.8 out of 5
9 Customer Ratings
5 Stars
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4 Stars
22%
3 Stars
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1 Stars
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  • Recombinant Mouse IL-2 Protein
    Name: Anonymous
    Application: In vitro bioactivity in cell culture
    Verified Customer | Posted 06/17/2021
  • Recombinant Mouse IL-2 Protein
    Name: Anonymous
    Application: In vitro bioactivity in cell culture
    Verified Customer | Posted 02/20/2021
  • Recombinant Mouse IL-2 Protein
    Name: Anonymous
    Application: Cell Proliferation
    Verified Customer | Posted 05/23/2020
    Recombinant Mouse IL-2 Protein 402-ML
  • Recombinant Mouse IL-2 Protein
    Name: Anonymous
    Application: In vitro bioactivity in cell culture
    Verified Customer | Posted 09/16/2019
    Recombinant Mouse IL-2 Protein 402-ML
  • Recombinant Mouse IL-2 Protein
    Name: Anonymous
    Application: Cell migration/motility
    Verified Customer | Posted 05/03/2018
  • Recombinant Mouse IL-2 Protein
    Name: Anonymous
    Application: Cell Proliferation
    Verified Customer | Posted 04/26/2018
  • Recombinant Mouse IL-2 Protein
    Name: Anonymous
    Application: In vitro bioactivity in cell culture
    Verified Customer | Posted 04/22/2018
  • Recombinant Mouse IL-2 Protein
    Name: Tima Thomas
    Application: In vitro bioactivity in cell culture
    Verified Customer | Posted 01/21/2016
  • Recombinant Mouse IL-2 Protein
    Name: SOjan Abraham
    Application: In vitro bioactivity in cell culture
    Verified Customer | Posted 01/21/2016

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FAQs for Recombinant Mouse IL-2 Protein

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    • Q: The source information for Recombinant Mouse IL-2 Protein (Catalog# 402-ML) states "Ala21-Gln169, with and without an N-terminal Met". What does "with and without an N-terminal Met" mean?
      A: When the signal peptide gets cleaved off during post-translational modification, sometimes the N-terminal methionine also gets cleaved off. This does not affect protein function.
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