Recombinant Mouse NOV/CCN3 Protein, CF
Recombinant Mouse NOV/CCN3 Protein, CF Summary
Ser26-Ile354, with a C-terminal 10-His tag
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS.|
|Reconstitution||Reconstitute at 100 μg/mL in sterile PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
NOV, also called CCN3, is one of six CCN (CYR61/CTGF/NOV) secreted proteins which share a common multimodular organization (1 - 4). NOV/CCN3 contains an N-terminal IGFBP domain that appears to be non-functional and a vWF type C and thrombospondin type I domain which mediate oligomerization and matrix interactions, respectively (1, 2). The C-terminal cysteine knot domain interacts with several partners, including the matrix protein fibulin 1C (5), Notch-1 (6), and CCN2, which it may heterodimerize (2). NOV/CCN3 also interacts with the gap junction protein Connexin43 and mediates suppression of proliferation (7). It also binds the calcium binding protein S100A4 and promotes calcium channel activation (8). The 354 amino acid (aa), 44 kDa human NOV/CCN3 shares 80% aa identity with mouse, rat and canine NOV/CCN3, and 78% aa identity with bovine NOV/CCN3. NOV/CCN3 also shows 38 - 50% aa identity with other family members including WISP proteins, except for WISP-2/CCN5 which lacks the cysteine knot (1). NOV/CCN3 is widely expressed developmentally, especially in muscle, endothelium, nervous system, adrenal cortex and chondrocytes (1 - 4). In transformed cells, a 32 kDa N-terminally truncated form lacks the signal sequence is localized to the nucleus. Truncation allows a C-terminal nuclear localization sequence to be active (9). Nuclear NOV/CCN3 acts as a transcriptional repressor but promotes proliferation, presumably by interfering with growth control (9). Full length NOV/CCN3 is a secreted matricellular protein which inhibits cell growth. Interaction of NOV/CCN3 with integrins alpha v beta 3 and alpha 5 beta 1 mediates endothelial cell adhesion, induces chemotaxis and promotes angiogenesis (10, 11). Over-expression of NOV/CCN3 downregulates myogenic genes such as MyoD (12).
- Perbal, B. (2004) Lancet 363:62.
- Perbal, B. (2006) Cell Commun. Signal. 4:3.
- Martinerie, C. et al. (1994) Oncogene 9:2729.
- Snaith, M. R. et al. (1996) Genomics 38:425.
- Perbal, B. et al. (1999) Proc. Natl. Acad. Sci. USA 96:869.
- Sakamoto, K. et al. (2002) J. Biol. Chem. 277:29399.
- Fu, C. T. et al. (2004) J. Biol. Chem. 279:36943.
- Li, C. L. et al. (2002) Mol. Pathol. 55:250.
- Planque, N. et al. (2006) J. Cell. Biochem. Apr. 5 [e-pub ahead of print].
- Lin, C. G. et al. (2003) J. Biol. Chem. 278:24200.
- Lin, C. G. et al. (2003) J. Biol. Chem. 280:8229.
- Calhabeu, F. et al. (2006) Exp. Cell. Res. 312:1876.
Citations for Recombinant Mouse NOV/CCN3 Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 5
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CCN3/NOV Regulates Proliferation and Neuronal Differentiation in Mouse Hippocampal Neural Stem Cells via the Activation of the Notch/PTEN/AKT Pathway
Authors: Luan, Y;Zhang, H;Ma, K;Liu, Y;Lu, H;Chen, X;Liu, Y;Zhang, Z;
International journal of molecular sciences
Sample Types: Whole Cells
Ascl2-Dependent Cell Dedifferentiation Drives Regeneration of Ablated Intestinal Stem Cells
Authors: K Murata, U Jadhav, S Madha, J van Es, J Dean, A Cavazza, K Wucherpfen, F Michor, H Clevers, RA Shivdasani
Cell Stem Cell, 2020-02-20;0(0):.
Sample Types: Whole Cells
Applications: Cell Culture
Regulatory T cells promote myelin regeneration in the central nervous system
Authors: Y Dombrowski, T O'Hagan, M Dittmer, R Penalva, SR Mayoral, P Bankhead, S Fleville, G Eleftheria, C Zhao, M Naughton, R Hassan, J Moffat, J Falconer, A Boyd, P Hamilton, IV Allen, A Kissenpfen, PN Moynagh, E Evergren, B Perbal, AC Williams, RJ Ingram, JR Chan, RJ Franklin, DC Fitzgerald
Nat. Neurosci, 2017-03-13;0(0):.
Sample Types: Cell Culture Supernates
Cancer stem cells regulate cancer-associated fibroblasts via activation of Hedgehog signaling in mammary gland tumors
Authors: G Valenti, HM Quinn, GJ Heynen, L Lan, JD Holland, R Vogel, A Wulf-Golde, W Birchmeier
Cancer Res, 2017-02-15;0(0):.
Sample Types: Whole Cells
CCN3 (NOV) is a negative regulator of CCN2 (CTGF) and a novel endogenous inhibitor of the fibrotic pathway in an in vitro model of renal disease.
Authors: Riser BL, Najmabadi F, Perbal B, Peterson DR, Rambow JA, Riser ML, Sukowski E, Yeger H, Riser SC
Am. J. Pathol., 2009-04-09;174(5):1725-34.
Applications: ELISA (Standard)
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