Recombinant Mouse PDGF-D Protein, CF
Recombinant Mouse PDGF-D Protein, CF Summary
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in HCl.|
|Reconstitution||Reconstitute at 250 μg/mL in 4 mM HCl.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Recombinant Mouse PDGF-D (Catalog # 9738-SB) stimulates cell proliferation of the NR6R-3T3 mouse fibroblast cell line. The ED50 for this effect is 15-90 ng/mL.
The platelet-derived growth factor (PDGF) family consists of four disulfide-linked homodimers and one heterodimer (PDGF-AB). These proteins regulate diverse cellular functions through interactions with PDGF R alpha and R beta (1, 2). Mature PDGF-DD associates with PDGF R beta and triggers signaling through PDGF R beta homodimers and PDGF R alpha / beta heterodimers (3-5). The mouse PDGF-DD cDNA encodes a 370 amino acid (aa) precursor that includes a 23 aa signal sequence, a 226 aa CUB domain, and a 121 aa PDGF/VEGF domain (3, 4). The PDGF/VEGF domain shares 27-35% aa sequence identity with the corresponding regions of other PDGF family members. Mouse PDGF-DD shares 91% and 98% aa sequence identity with human and rat PDGF-DD, respectively. PDGF-DD is secreted as a 100 kDa latent homodimer which is activated by proteolysis to release a 35 kDa bioactive protein containing the disulfide-linked dimer of PDGF/VEGF domain (3, 4, 6, 7). A splice variant of PDGF-DD has a 6 aa deletion near the N-terminus. A 72 aa deletion within the PDGF/VEGF domain generates an inactive protein in mouse but has not been detected in human (8). PDGF-DD is widely expressed in embryonic and adult tissues (3, 9, 10), and PDGF R beta is expressed in a generally complementary pattern (9, 11, 12). PDGF-DD functions as a growth factor for renal artery smooth muscle cells and lens epithelial cells, and as a macrophage chemoattractant (5, 9-11). PDGF-DD is over-expressed in and contributes to several disease states, including renal and hepatic fibrosis, mesangial proliferative glomerulopathy, pulmonary lymphoid infiltration, and many cancers (6, 11-15). PDGF-DD functions in both paracrine and autocrine manners (6, 7, 14).
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