Recombinant Porcine IL-8/CXCL8 Protein

Formulations:
Catalog # Availability Size / Price Qty
535-IN-025
Product Details
Citations (4)
FAQs
Reviews (2)

Recombinant Porcine IL-8/CXCL8 Protein Summary

Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to induce myeloperoxidase release from cytochalasin B-treated human neutrophils. Schröder, J.M. et al. (1987) J. Immunol. 139:3474. The ED50 for this effect is 0.4‑0.6 µg/mL. Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with human CXCR2. The ED50 for this effect is 1-5 ng/mL.
Source
E. coli-derived porcine IL-8/CXCL8 protein
Ala26-Gln104
Accession #
N-terminal Sequence
Analysis
Ala26
Predicted Molecular Mass
9.4 kDa

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

535-IN

Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Reconstitution Reconstitute at 25 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

535-IN/CF

Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-8/CXCL8

Interleukin-8 (IL-8), also known as CXCL8, GCP-1, and NAP-1, is a widely expressed proinflammatory member of the CXC family of chemokines. This 8-9 kDa chemokine circulates as a monomer, homodimer, and heterodimer with CXCL4/PF4 (1, 2). Its oligomerization is modulated by interactions with matrix and cell surface glycosaminoglycans (GAGs) (3). Mature porcine CXCL8 shares 82%, 78%, and 65% amino acid (aa) sequence identiity with canine, feline, and human CXCL8 (4). There is no CXCL8 gene counterpart in rodent. N-terminal truncation of CXCL8 by multiple proteases generates a range of shorter forms (5). The bioactivity of CXCL8 is regulated by these truncations and also by CXCL8 citrullination (6). CXCL8 effects are mediated through CXCR1/IL-8 RA, which is also used by CXCL6, and through CXCR2/IL-8 RB, which is used by multiple CXC chemokines (1). These receptors associate into functional homodimers and heterodimers with each other (7). Through both CXCR1 and CXCR2, CXCL8 promotes neutrophil adhesion to the vascular endothelium and migration to sites of inflammation (8). It triggers the antimicrobial activation of neutrophils through CXCR1 (9). CXCL8 also binds to Serpin A1/alpha-1 Antitrypsin, and this prevents CXCL8 interaction with CXCR1 (10). CXCL8 is up-regulated in atherosclerotic lesions and other cardiac pathologies where it exacerbates inflammatory tissue damage (11). In addition, it induces VEGF expression, vascular endothelial cell proliferation, angiogenesis, and tumor cell invasiveness (12-15).

References
  1. Lazennec, G. and A. Richmond (2010) Trends Mol. Med. 16:133.
  2. Nesmelova, I.V. et al. (2005) J. Biol. Chem. 280:4948.
  3. Pichert, A. et al. (2012) Biomatter 2:142.
  4. Goodman, R.B. et al. (1992) Biochemistry 31:10483.
  5. Mortier, A. et al. (2008) Pharmacol. Ther. 120:197.
  6. Proost, P. et al. (2008) J. Exp. Med. 205:2085.
  7. Munoz, L.M. et al. (2009) J. Immunol. 183:7337.
  8. Gerszten, R.E. et al. (1999) Nature 398:718.
  9. Jones, S.A. et al. (1996) Proc. Natl. Acad. Sci. USA 93:6682.
  10. Bergin, D.A. et al. (2010) J. Clin. Invest. 120:4236.
  11. Apostolakis, S. et al. (2009) Cardiovasc. Res. 84:353.
  12. Martin, D. et al. (2009) J. Biol. Chem. 284:6038.
  13. Li, A. et al. (2005) Angiogenesis 8:63.
  14. Waugh, D.J. and C. Wilson (2008) Clin. Cancer Res. 14:6735.
  15. Fernando, R.I. et al. (2011) Cancer Res. 71:5296.
Long Name
Interleukin 8
Entrez Gene IDs
3576 (Human); 396880 (Porcine); 403850 (Canine); 493836 (Feline)
Alternate Names
3-10C; AMCF-I; C-X-C motif chemokine 8; CXCL8; CXCL8SCYB8; Emoctakin; GCP1; GCP-1TSG-1; IL8; IL-8; interleukin 8; K60; LAI; LECT; LUCT; LYNAP; MDNCF; MDNCFb-ENAP; member 8; MONAP; MONAPGCP1; NAF; NAP1; NAP-1NAP1; NCF; Neutrophil-activating protein 1; Protein 3-10C; T cell chemotactic factor; T-cell chemotactic factor; TCF; TSG1

Citations for Recombinant Porcine IL-8/CXCL8 Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Protective Role of Passively Transferred Maternal Cytokines against Bordetella pertussis Infection in Newborn Piglets
    Authors: S Elahi, DR Thompson, J Van Kessel, LA Babiuk, V Gerdts
    Infect. Immun., 2017;85(4):.
    Species: Porcine
    Sample Types: Serum
    Applications: ELISA (Standard)
  2. Increase in chemokines CXCL10 and CCL2 in blood from pigs infected with high compared to low virulence African swine fever virus isolates.
    Authors: Fishbourne E, Hutet E, Abrams C, Cariolet R, Le Potier M, Takamatsu H, Dixon L
    Vet Res, 2013;44(0):87.
    Species: Porcine
    Sample Types: Plasma
    Applications: Bioassay
  3. Simultaneous detection of porcine proinflammatory cytokines using multiplex flow cytometry by the xMAP technology.
    Authors: Johannisson A, Jonasson R, Dernfalk J, Jensen-Waern M
    Cytometry A, 2006;69(5):391-5.
    Species: N/A
    Sample Types: N/A
    Applications: ELISA (Standard)
  4. Acute hyperinsulinemia restrains endotoxin-induced systemic inflammatory response: an experimental study in a porcine model.
    Authors: Brix-Christensen V, Andersen SK, Andersen R, Mengel A, Dyhr T, Andersen NT, Schmitz O, Orskov H, Tonnesen E
    Anesthesiology, 2004;100(4):861-70.
    Species: N/A
    Sample Types: N/A
    Applications: ELISA (Standard)

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Reviews for Recombinant Porcine IL-8/CXCL8 Protein

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Recombinant Porcine IL-8/CXCL8 Protein
By Anonymous on 11/26/2018
Application: MSD assay

The recombinant porcine IL-8 protein was used as the calibration reagent in MSD assay with biotinylated MAB535 (capture) and Sulfo-Tag labeled AF-535 (detection) antibodies.


Recombinant Porcine IL-8/CXCL8 Protein
By Anonymous on 11/08/2017
Application: Immunoassay Standard

The antibody MAB5351 was used as the capture antibody in a sandwich ELISA for Porcine IL-8 along with the detection antibody, AF535. The calibrator for the assay was recombinant porcine IL-8, 535-IN. It was sensitive and specific and the recombinant IL-8 was stable.