Key Product Details

Species Reactivity

Rat

Applications

Western Blot

Label

CoraFluor 1

Antibody Source

Polyclonal Goat IgG
View all Reg3B Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant rat Reg3B
Glu27-Gly175
Accession # P25031

Specificity

Detects rat Reg3B in direct ELISAs and Western blots. In direct ELISAs, approxmately 75% cross-reactivity with recombinant mouse (rm) Reg3B, and approximately 20% cross-reactivity with rmReg3G is observed, and less than 2% cross-reactivity with recombinant human (rh) Reg3A, rmReg1, rmReg2, rhReg4, rhReg1A, rmReg3A, and rmReg3D is observed. In Western blots, approximately 40% cross-reactivity with rmReg3B is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Description

This conjugate is made on demand and is not held in inventory. Each order represents a unique lot. All conjugates are generated using validated conjugation protocols and meet strict degree of labeling (F/P) specifications; additional information is available upon request. The antibody concentration is provided on the individual product label.

For specialized conjugation needs, like large quantities, specific concentrations, and defined F/P ratios, bulk and custom antibody conjugation services are available.

Scientific Data Images for Reg3B Antibody [CoraFluor™ 1]

Reg3B Antibody [CoraFluor™ 1]

Product Feature: CoraFluor Probes for TR-FRET

CoraFluor™ 1, amine reactive (Catalog:7920) and CoraFluor™ 2, amine reactive (Catalog # 7950) are terbium-based probes that have been developed for use as TR-FRET donors. They emit wavelengths compatible with commonly used fluorescent acceptor dyes such as BODIPY® (or BDY) and Janelia Fluor® dyes, FITC (Catalog # 5440), TMR and Cyanine 5 (Catalog # 5436). CoraFluor™ fluorescence is brighter and more stable in biological media than existing TR-FRET donors, leading to enhanced sensitivity and improved data generation. CoraFluor™ 1 exhibits excitation upon exposure to a 337 nm UV laser.

Applications for Reg3B Antibody [CoraFluor™ 1]

Application
Recommended Usage

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Recommended applications are based on validated applications from the unconjugated base product AF1996. This conjugated antibody is not kept in inventory and is made to order using validated conjugation protocols.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark. Do not freeze.

Background: Reg3B

The Reg family constitutes a subset of the calcium-dependent C-type lectin superfamily. These small, secreted proteins have been implicated in a range of physiological processes including acting as acute phase reactants, lectins, survival/growth factors for insulin-producing pancreatic beta-cells, neural cells, and epithelial cells of the digestive system. Studies also indicate a role for Reg family members in tumor formation and indicate their potential for use as biomarkers of carcinogenesis.

Reg3B (regenerating islet-derived protein III beta; also PAP-I and HIP) is a secreted 17 kDa member of the Reg gene family, C-type lectin gene superfamily. It is found in pancreatic acinar cells, intestinal enterocytes, and developing spinal motoneurons. Reg3B may play a role in intestinal epithelial cell renewal. Mouse Reg3B contains one C-type lectin domain (aa 38 - 173). Mature mouse Reg3B is 59%, 71% and 51% aa identical to the products of three other Reg3 named genes termed Reg III alpha, gamma and delta, respectively. Mature mouse Reg3B is also 91% and 67% aa identical to rat and human Reg3B, respectively.

Long Name

Regenerating Islet-derived IIIb

Alternate Names

PAP1

Gene Symbol

REG3A

Additional Reg3B Products

Product Documents for Reg3B Antibody [CoraFluor™ 1]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Reg3B Antibody [CoraFluor™ 1]

Sold for research purposes only under agreement from Massachusetts General Hospital, US Patent No. 12,134,721. A license is required for development of new commercial assay platforms (e.g., microarrays, microfluidics), diagnostic use or other unlicensed uses. Please contact Technical Support for further details: techsupport@bio-techne.com.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

C-type Lectin Family
Diabetes

Customer Reviews for Reg3B Antibody [CoraFluor™ 1]

There are currently no reviews for this product. Be the first to review Reg3B Antibody [CoraFluor™ 1] and earn rewards!

Have you used Reg3B Antibody [CoraFluor™ 1]?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 for a review with an image

$10/€7/£6/$10CAN/¥1110 for a review without an image

Submit a review
Amazon Gift Card

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies