RENT1/UPF1/hUPF1 Antibody

Novus Biologicals | Catalog # NBP1-05967

Novus Biologicals
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Applications

Validated:

Western Blot, Immunoprecipitation

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all RENT1/UPF1 Primary Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 1079 and 1129 of human regulator of nonsense transcripts 1 using the numbering given in entry NP_002902.2 (GeneID 5976).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for RENT1/UPF1/hUPF1 Antibody

Western Blot: RENT1/UPF1/hUPF1 Antibody [NBP1-05967]

Western Blot: RENT1/UPF1/hUPF1 Antibody [NBP1-05967]

Western Blot: RENT1/UPF1/hUPF1 Antibody [NBP1-05967] - Whole cell lysate from HeLa, (15 ug), 293T, (15 ug), Jurkat, (15 ug), mouse TCMK-1, (50 ug), and mouse NIH3T3 (50 ug) cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RENT1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Western Blot: RENT1/UPF1/hUPF1 Antibody [NBP1-05967]

Western Blot: RENT1/UPF1/hUPF1 Antibody [NBP1-05967]

Western Blot: RENT1/UPF1/hUPF1 Antibody [NBP1-05967] - Whole cell lysate from HeLa and mouse NIH3T3.
Immunoprecipitation: RENT1/UPF1/hUPF1 Antibody [NBP1-05967]

Immunoprecipitation: RENT1/UPF1/hUPF1 Antibody [NBP1-05967]

Immunoprecipitation: RENT1/UPF1/hUPF1 Antibody [NBP1-05967] - Detection of human RENT1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-RENT1 antibody NBP1-05967 used for IP at 3 ug per reaction. RENT1 was also immunoprecipitated by rabbit anti-RENT1 antibody NB100-370. For blotting immunoprecipitated RENT1, NBP1-05967 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

Applications for RENT1/UPF1/hUPF1 Antibody

Application
Recommended Usage

Immunoprecipitation

2 - 10 ug/mg lysate

Western Blot

1:2000-1:10000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: RENT1/UPF1

This gene encodes a protein that is part of a post-splicing multiprotein complex involved in both mRNA nuclear export and mRNA surveillance. mRNA surveillance detects exported mRNAs with truncated open reading frames and initiates nonsense-mediated mRNA decay (NMD). When translation ends upstream from the last exon-exon junction, this triggers NMD to degrade mRNAs containing premature stop codons. This protein is located only in the cytoplasm. When translation ends, it interacts with the protein that is a functional homolog of yeast Upf2p to trigger mRNA decapping. Use of multiple polyadenylation sites has been noted for this gene. [provided by RefSeq]

Long Name

Regulator of Nonsense Transcripts 1

Alternate Names

NORF1, UPF1

Entrez Gene IDs

5976 (Human); 19704 (Mouse)

Gene Symbol

UPF1

UniProt

Q92900 (Human)

Additional RENT1/UPF1 Products

Product Documents for RENT1/UPF1/hUPF1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for RENT1/UPF1/hUPF1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for RENT1/UPF1/hUPF1 Antibody

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