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Key Product Details
Species Reactivity
Validated:
Human
Predicted:
Mouse (93%). Backed by our 100% Guarantee.
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
A portion of amino acids 1-50 of human Rictor was used as the immunogen.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
Novus Biologicals Rabbit Rictor Antibody - BSA Free (NB100-56427) is a polyclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for Rictor Antibody - BSA Free
Western Blot: Rictor Antibody [NB100-56427]
Western Blot: Rictor Antibody [NB100-56427] - Analysis of Rictor in Jurkat cell lysate using this antibody.Applications for Rictor Antibody - BSA Free
Application
Recommended Usage
Western Blot
5-7 ug/ml
Reviewed Applications
Read 1 review rated 3 using NB100-56427 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS containing 0.2% gelatin
Format
BSA Free
Preservative
0.05% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Rictor
Long Name
Rapamycin-insensitive Companion of mTOR
Alternate Names
mAVO3, Pianissimo
Gene Symbol
RICTOR
UniProt
Additional Rictor Products
Product Documents for Rictor Antibody - BSA Free
Product Specific Notices for Rictor Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
Customer Reviews for Rictor Antibody - BSA Free (1)
3 out of 5
1 Customer Rating
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Application: Western BlotSample Tested: Adipose tissueSpecies: MouseVerified Customer | Posted 10/23/2019Mouse white adipose tissue was homogenised and protein content was quantified by a BCA assay. Twenty micrograms of protein were resolved on a 4-12% Bis-Tris gel and transferred to nitrocellulose membranes. Membranes were probed with primary antibody Rictor diluted 1:1000 in 5% BSA 2, before incubation with anti rabbit secondary horseradish peroxidase-conjugated antibody 1:5000. Blots were visualised with Immobilon Western Chemiluminescence HRP Substrate and imaged with Syngene chemiluminescence imaging system.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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