Key Product Details
Validated by
Independent Antibodies
Species Reactivity
Validated:
Human, Mouse
Predicted:
Bovine (100%), Rat (100%). Backed by our 100% Guarantee.
Applications
Western Blot, Simple Western, Immunoprecipitation
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
This antibody maps to a region between residue 243 and 293 of human Ribosomal Protein S2 using the numbering given in entry NP_002943.2 (GeneID 6187).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for RPS2 Antibody - BSA Free
Western Blot: RPS2 Antibody [NBP2-14853]
Western Blot: RPS2 Antibody [NBP2-14853] - Detection of human and mouse RPS2 by western blot (h and m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 ug), Jurkat (J; 50 ug) and mouse NIH 3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-RPS2 antibody NBP2-14853 used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. RPS2 was also immunoprecipitated by rabbit anti-RPS2 antibody NBP2-14852, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 10 seconds (A) and 3 seconds (B).Applications for RPS2 Antibody - BSA Free
Application
Recommended Usage
Immunoprecipitation
2-10 ug/mg lysate
Simple Western
1:25
Western Blot
1:2000-1:10000
Application Notes
Western blot of lysates performed using standard western blot reagents and 4-20% SDS-PAGE.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: RPS2
Alternate Names
LLREP3, MGC102851, MGC117344, MGC117345,40S ribosomal protein S4, OK/KNS-cl.6,40S ribosomal protein S2, Protein LLRep3, ribosomal protein S2, RPS4
Gene Symbol
RPS2
UniProt
Additional RPS2 Products
Product Documents for RPS2 Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for RPS2 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- Immunoprecipitation Protocol
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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