Se-NADA

Tocris Bioscience | Catalog # 7449

Orange fluorescent benzoselenadiazole D-amino acid (FDAA) for imaging bacteria; also photosensitizer
Tocris Bioscience
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Key Product Details

Description

Orange fluorescent benzoselenadiazole D-amino acid (FDAA) for imaging bacteria; also photosensitizer

Wavelength

Excitation = 470 nm, Emission = 590 nm

Product Description

Se-NADA is an orange fluorescent benzoselenadiazole D-amino acid (FDAA) for imaging bacteria. It is covalently incorporated into peptidoglycans in the cell walls of live bacteria. It also acts as a photosensitizer, bringing about bacterial cell death on exposure to visible light. Se-NADA is effective against both Gram-positive and Gram-negative bacteria. Ex/Em = 470/590 nm.

Scientific Data Images for Se-NADA

Application of Se-NADA in B subtilis bacteria cells

Application of Se-NADA in B subtilis bacteria cells.

Fluorescence microscopy image of B subtilis bacterial cells treated with SeNADA (250 μM, red) proving incorporation of the D-amino acid into the peptidoglycans of the cell wall. Image kindly provided by Dr Erkin Kuru, Department of Genetics, Harvard Medical School, Boston.

Licensing Information

Sold under license from the University of Edinburgh

Optical Data

Se-NADA Dye Spectra
Emission Color Orange
λabs 470 nm
λem 590 nm
Cell Permeable Yes
Application Fluorescence Microscopy

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

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Product Specifications for Se-NADA

Molecular Weight

366.62

Formula

C9H9N5O4Se.HCl

Storage

Store at -20°C

Purity

≥95% (HPLC)

Chemical Name

3-((7-Nitro-2,1,3-benzoselenadiazol-4-yl)amino)-D-alanine hydrochloride

CAS Number

2571574-70-2

InChI Key

NGEWTJCJCRCRQS-PGMHMLKASA-N

SMILES

N[C@@H](C(O)=O)CNC1=CC=C(N(=O)=O)C2=N[Se]N=C12.Cl

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

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Solubility

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 3.67 10

Preparing Stock Solutions for Se-NADA

The following data is based on the product molecular weight 366.62.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.1 mM 27.28 mL 136.38 mL 272.76 mL
0.5 mM 5.46 mL 27.28 mL 54.55 mL
1 mM 2.73 mL 13.64 mL 27.28 mL
5 mM 0.55 mL 2.73 mL 5.46 mL

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Background References

References are publications that support the biological activity of the product.

Product Documents for Se-NADA

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Se-NADA

For research use only

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