Key Product Details

Species Reactivity

Mouse

Applications

Western Blot

Label

PerCP (Excitation = 490 nm, Emission = 675 nm)

Antibody Source

Polyclonal Goat IgG
View all Siglec-3/CD33 Primary Antibodies »

Product Specifications

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse Siglec-3/CD33
Gln17-Val244
Accession # Q63994

Specificity

Detects mouse Siglec-3 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 10% cross-reactivity with recombinant human (rh) Siglec-7 and rhSiglec-9 and less than 5% cross-reactivity with recombinant mouse (rm) Siglec-F, rmSiglec-2, rhSiglec‑3, rhSiglec-5, and rhSiglec-10 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Description

This conjugate is made on demand and is not held in inventory. Each order represents a unique lot. All conjugates are generated using validated conjugation protocols and meet strict degree of labeling (F/P) specifications; additional information is available upon request. The antibody concentration is provided on the individual product label.

For specialized conjugation needs, like large quantities, specific concentrations, and defined F/P ratios, bulk and custom antibody conjugation services are available.

Applications for Siglec-3/CD33 Antibody [PerCP]

Application
Recommended Usage

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Recommended applications are based on validated applications from the unconjugated base product AF2220. This conjugated antibody is not kept in inventory and is made to order using validated conjugation protocols.

Reviewed Applications

Read 1 review rated 5 using AF2220C in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: Siglec-3/CD33

CD33 is a 67 kDa type I transmembrane glycoprotein and a member of the sialoadhesin family of cell surface receptors. It is absent from pluripotent stem cells but appears on myelomonocytic precursors after CD34. It then continues to be expressed on both the myeloid and monocyte lineages, although it is absent on granulocytes. While it has been reported that CD33 can function as a sialic acid-dependent cell adhesion molecule, cells expressing CD33 require desialylation before they can bind cells bearing the appropriate sialoglycoconjugates. This suggests that inhibitory cis interactions may regulate or block any adhesion function. (1-4)

Long Name

Sialic Acid Binding Ig-like Lectin 3

Alternate Names

CD33, gp67, Siglec3

Gene Symbol

CD33

Additional Siglec-3/CD33 Products

Product Documents for Siglec-3/CD33 Antibody [PerCP]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Siglec-3/CD33 Antibody [PerCP]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for Siglec-3/CD33 Antibody [PerCP] (1)

5 out of 5
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Customer Images

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  • CD33 expression in bone marrow
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: Bone marrow cells
    Species: Mouse
    Verified Customer | Posted 08/02/2026
    Bone marrow cells stained with CD33
    Bone marrow cells were stained directly in 1% BSA at 1:200 dilution in ice for 30 min. The cells were washed and run in flow cytometry.
    Siglec-3/CD33 Antibody [PerCP] AF2220C

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