Simple Plex Discovery Human SPARC Cartridge

R&D Systems | Catalog # SPCKB-PS-016134

Simple Plex Discovery Human SPARC assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer. Sample type indicated by PS (Plasma/Serum).
R&D Systems

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Key Product Details

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Cell Culture Supernates (10 µL), Serum (10 µL), EDTA Plasma (10 µL), Platelet-poor EDTA Plasma (10 µL), Heparin Plasma (10 µL), Platelet-poor Heparin Plasma (10 µL), Cerebrospinal Fluid (10 µL)

Sensitivity

6.32 pg/mL

Assay Range

76.8-46856 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Platelet-poor EDTA Plasma, Heparin Plasma, Platelet-poor Heparin Plasma, Cerebrospinal Fluid)

Simple Plex Discovery Human SPARC Cartridge Summary

Simple Plex Discovery assay is for the detection of human Secreted Protein, Acidic and Rich in Cysteine (SPARC) in cell culture supernatant (CCS), serum, plasma, platelet-poor plasma (EDTA/Heparin), and cerebrospinal fluid (CSF) samples using Sample Diluent SD13. 

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View all SPARC Simple Plex »

Compatible Instruments

Ella Automated Immunoassay System

Ella Automated Immunoassay System

Efficiently analyze biomarkers with Ella's advanced technology, providing accurate, precise results in under 90 minutes. Quantify up to 8 targets per assay through a streamlined automated workflow. Choose from >350 highly validated Simple Plex assays or Simple Plex discovery assays.
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Product Specifications

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge with 3 Glass Nano Reactors (GNR)

Species

Human

Specificity

Natural and recombinant human SPARC.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

精度

Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one SPARC assay.

Inter-Assay Precision (Precision between assays) Replicates of each SPARC control were tested in multiple assays performed by at least three technicians using two lots of reagents for human SPARC.

Cell Culture Supernates, Cerebrospinal Fluid, EDTA Plasma, Heparin Plasma, Platelet-poor EDTA Plasma, Platelet-poor Heparin Plasma, Serum

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 2
n 16 16 22 22
Mean (pg/mL) 363 17871 367 19043
Standard Deviation 13.3 933 32.9 1301
CV% 3.7 5.2 9.0 6.8

Recovery for Simple Plex Discovery Human SPARC Cartridge

Recovery for the human SPARC assays was evaluated at three different spiked concentrations of SPARC protein.

Sample Type Average % Recovery Range %
Cell Culture Media (n=2) 102 99-105

Linearity

Samples containing and/or spiked with high concentrations of SPARC were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Simple Plex Discovery Human SPARC Assay Linearity

Scientific Data Images for Simple Plex Discovery Human SPARC Cartridge

Simple Plex Discovery Human SPARC Assay Standard Curve

Simple Plex Discovery Human SPARC Assay Standard Curve

Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Discovery Human SPARC Assay. Assay range is 76.8 - 46,856 pg/mL.

Background: SPARC

SPARC, also known as Osteonectin and Basement Membrane protein BM-40, is a phosphorylated glycoprotein with calcium-binding domains. It is secreted by endothelial cells and is present in large amounts in the parietal endoderm of mouse embryos and in human placenta. SPARC may be involved in cell proliferation, repair of tissue damage, and morphogenic processes such as re-modeling of the extracellular matrix.

Long Name

Secreted Protein Acidic and Rich in Cysteine

Alternate Names

BM-40, Osteonectin

Entrez Gene IDs

6678 (Human); 20692 (Mouse)

Gene Symbol

SPARC

Additional SPARC Products

Product Documents for Simple Plex Discovery Human SPARC Cartridge

Certificate of Analysis

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Product Specific Notices for Simple Plex Discovery Human SPARC Cartridge

For research use only

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