Simple Plex Human Caspase-1 Cartridge (2nd Gen)

Catalog #: SPCKB-PS-003613 Datasheet / COA / SDS

Simple Plex Human Caspase-1 assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer

Catalog #	Availability	Size / Price	Qty
SPCKB-PS-003613
SPCKA-PS-003613
SPCKC-PS-003613

Control Products Available

898569: Simple Plex Control for Human Caspase-1

Ella Automated ELISA Platform and Simple Plex Human Caspace-1 Assay

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Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Simple Plex Human Caspase-1 Cartridge (2nd Gen) Summary

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Assay Length

80 minutes

Sample Type & Volume Required Per Well*

Cell Culture Supernates (25 uL), Serum (25 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL), Cerebrospinal Fluid (25 uL)

Sensitivity

0.04 pg/mL

Assay Range

0.1 - 422 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Cerebrospinal Fluid)

Specificity

Natural and recombinant human Caspase-1.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

Product Summary

Simple Plex assay is for the detection of human Caspase-1/ICE in cell culture supernatant (CCS), serum, plasma (EDTA/Heparin, and cerebrospinal fluid (CSF) in Sample Diluent SD13.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one Caspase-1 assay

Inter-Assay Precision (Precision between assays) Replicates of each Caspace-1 control were tested in multiple assays performed by at least three technicians using two lots of reagents for human Caspace-1

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Cerebrospinal Fluid

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	29	30
Mean (pg/mL)	2.3	117	2.28	125
Standard Deviation	0.104	4.09	0.259	12.2
CV%	4.5	3.5	11.4	9.8

Recovery

Recovery for the human Caspace-1 assays was evaluated at three different spiked concentrations of Caspace-1 protein.

Sample Type	Average % Recovery	Range %
Cerebrospinal Fluid (n=5)	116	103-123
EDTA Plasma (n=4)	90	78-101
Heparin Plasma (n=4)	90	82-97
Serum (n=4)	91	83-97

Linearity

Samples containing and/or spiked with high concentrations of Caspace-1 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Scientific Data

N/A

Simple Plex Human Caspace-1 Assay Standard Curve

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Human Caspase-1 Simple Plex Assay Standard Curve Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Human Caspace-1 Assay. Assay range is 0.11-422 pg/mL

Product Datasheets

Product Datasheet

COA

SDS

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Caspase-1

Caspases are a family of cytosolic aspartate-specific cysteine proteases involved in the initiation and execution of apoptosis. They are expressed as latent zymogens and are activated by an autoproteolytic mechanism or by processing by other proteases (frequently other caspases). Human caspases can be subdivided into three functional groups: cytokine activation (caspase-1, -4, -5, and -13), apoptosis initiation (caspase-2, -8, -9, -and -10), and apoptosis execution (caspase-3, -6, and -7).

Caspases are regulated by a variety of stimili, including APAF1, CFLAR/FLIP, NOL3/ARC, and members of the inhibitor of apoptosis (IAP) family such as BIRC1/NAIP, BIRC2/cIAP-1, BIRC3/cIAP-2, BIRC4/XIAP, BIRC5/Survivin, and BIRC7/Livin. IAP activity is modulated by DIABLO/SMAC or PRSS25/HTRA2/Omi. Cell-permeable and irreversible peptide inhibitors are also available for different caspases.

Entrez Gene IDs:

834 (Human)

Alternate Names:

CASP1 nirs variant 1; CASP1; CASP-1; caspase 1, apoptosis-related cysteine peptidase; Caspase-1 subunit p10; Caspase-1 subunit p20; Caspase1; Caspase-1; EC 3.4.22.36; EC:3.4.22.36; ICE; IL-1 beta-converting enzyme; IL1BC; IL-1BC; IL1BCE; IL1B-convertase; interleukin 1, beta, convertase; interleukin 1-B converting enzyme; Interleukin-1 beta convertase; Interleukin-1 beta-converting enzyme; p45