Simple Plex Human GDF-8/Myostatin Cartridge New

Catalog #: SPCKB-PS-011933 Datasheet / COA / SDS

Simple Plex Human GDF-8 assay kit for use on Ella instrument. Contains cartridge, Sample Diluent SD63, and Wash Buffer

Catalog #	Availability	Size / Price	Qty
SPCKB-PS-011933
SPCKA-PS-011933
SPCKC-PS-011933

Control Products Available

898090: Simple Plex Control for Human/Mouse/Rat GDF-8

Simple Plex Human/Mouse/Rat GDF-8 Ella Assay

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Summary Sample Values Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Simple Plex Human GDF-8/Myostatin Cartridge Summary

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL)

Sensitivity

0.41 pg/mL

Assay Range

1.9 - 18,091 pg/mL (Serum, EDTA Plasma, Heparin Plasma)

Specificity

Natural and recombinant GDF-8.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

Interference observed with 1 or more available related molecules.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

Sample Values

Activated serum and plasma samples require a further 8-fold dilution (16 for human heparin) after activation with Sample Diluent.

Product Summary

Simple Plex assay for the detection of human, mouse, and rat Growth Differentiation Factor 8 (GDF-8) in serum and plasma (EDTA/Heparin) in Sample Diluent SD63.

Precision

Intra-Assay Precision (Precision within an assay) Multiple replicates of each control were tested in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple runs performed by at least three technicians using two lots of reagents

Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	68	65
Mean (pg/mL)	224	9507	140	6355
Standard Deviation	6.3	460	14.9	574
CV%	2.8	4.8	10.7	9

Recovery

Recovery was not necessary as GDF-8 shows 5 points of natural linearity.

Linearity

Samples containing and/or spiked with high concentrations of GDF-8 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Simple Plex Mouse GDF-8 Ella Assay Linearity

Simple Plex Rat GDF-8 Ella Assay Linearity

Scientific Data

N/A

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Simple Plex Human/Mouse/Rat GDF-8 Ella Assay Standard Curve

Product Datasheets

Product Datasheet

COA

SDS

Preparation and Storage

Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: GDF-8/Myostatin

Growth/differentiation factors (GDF-1 to GDF-15) are members of the BMP family of TGF-beta superfamily proteins. They are produced as inactive preproproteins which are then cleaved and assembled into active secreted homodimers. GDF dimers are disulfide-linked with the exception of GDF-3 and -9. GDF proteins are important during embryonic development, particularly in the skeletal, nervous, and muscular systems.

Long Name:

Growth Differentiation Factor 8

Entrez Gene IDs:

2660 (Human); 17700 (Mouse)

Alternate Names:

GDF8; GDF-8; GDF8growth differentiation factor 8; growth/differentiation factor 8; MSLHP; MSTN; Myostatin