Simple Plex Human Progranulin/PGRN Cartridge

R&D Systems | Catalog # SPCKB-PS-016248

Simple Plex Human Progranulin/PGRN assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer. Sample type indicated by PS (Plasma/Serum).
R&D Systems

Customize This Cartridge

Simple Plex cartridges are custom built to fit your needs. Choose from over 350 assays spanning human, mouse, rat, and non-human primate. Just select your sample type, cartridge size (16, 32, or 72 samples), and up to 8 analytes per cartridge. Customize this cartridge.

Customize This Cartridge

Key Product Details

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Cell Culture Supernates (10 µL), Serum (25 µL), EDTA Plasma (25 µL), Heparin Plasma (25 µL), Saliva (25 µL), Urine (25 µL), Human Milk (25 µL), Cerebrospinal Fluid (25 µL)

Sensitivity

4.52 pg/mL

Assay Range

21-200000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Urine, Human Milk, Cerebrospinal Fluid)

Simple Plex Human Progranulin/PGRN Cartridge Summary

Simple Plex assay is for the detection of human Progranulin/PGRN in cell culture supernatant (CCS), serum, plasma (EDTA/Heparin), cerebrospinal fluid (CSF), saliva, urine, and human milk in Sample Diluent SD13. 

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View all Progranulin/PGRN Simple Plex »

Compatible Instruments

Ella Automated Immunoassay System

Ella Automated Immunoassay System

Efficiently analyze biomarkers with Ella's advanced technology, providing accurate, precise results in under 90 minutes. Quantify up to 8 targets per assay through a streamlined automated workflow. Choose from >350 highly validated Simple Plex assays or Simple Plex discovery assays.
了解更多

Product Specifications

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge with 3 Glass Nano Reactors (GNR)

Species

Human

Specificity

Natural and recombinant human Progranulin.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

精度

Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one Progranulin/PGRN assay.

Inter-Assay Precision (Precision between assays) Replicates of each Progranulin/PGRN control were tested in multiple assays performed by at least three technicians using two lots of reagents for human Progranulin/PGRN.

Cell Culture Supernates, Cerebrospinal Fluid, EDTA Plasma, Heparin Plasma, Human Milk, Saliva, Serum, Urine

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 2
n 16 16 49 43
Mean (pg/mL) 1294 65001 1209 62818
Standard Deviation 66.0 1388 132 4030
CV% 5.1 2.1 10.9 6.4

Recovery for Simple Plex Human Progranulin/PGRN Cartridge

Recovery for the human Progranulin/PGRN assays was evaluated at three different spiked concentrations of Progranulin/PGRN protein.

Sample Type Average % Recovery Range %
Cell Culture Media (n=2) 100 99-102

Linearity

Samples containing and/or spiked with high concentrations of Progranulin/PGRN were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Simple Plex Human Progranulin/PGRN Assay Linearity

Scientific Data Images for Simple Plex Human Progranulin/PGRN Cartridge

Simple Plex Human Progranulin/PGRN Assay Standard Curve

Simple Plex Human Progranulin/PGRN Assay Standard Curve

Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Human Progranulin/PGRN Assay. Assay range is 21.0-200,000 pg/mL.

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Progranulin/PGRN

Progranulin, also known as Acrogranin, PCDGF, and Epithelin/Granulin Precursor, is a widely expressed glycoprotein that can undergo proteolysis to generate numerous Granulin peptides. Progranulin plays a role in the regulation of mesothelial differentiation, macrophage development, sexual differentiation of the brain, cartilage proliferation, wound repair, and the development of rheumatoid arthritis and osteoarthritis. It is often upregulated in cancer and contributes to tumor progression. Progranulin upregulation or mutation is associated with several neurological disorders including frontotemporal dementia, Creutzfeldt-Jakob disease, Alzheimer's disease, and motor neuron disease.

Alternate Names

Acrogranin, GEP, GP88, GRN, PCDGF, PEPI, PGRN, Proepithelin

Entrez Gene IDs

2896 (Human); 14824 (Mouse)

Gene Symbol

GRN

Additional Progranulin/PGRN Products

Product Documents for Simple Plex Human Progranulin/PGRN Cartridge

Certificate of Analysis

To download a Certificate of Analysis, please enter your Simple Plex catalog number and lot number in the search boxes below.

Product Specific Notices for Simple Plex Human Progranulin/PGRN Cartridge

For research use only

Related Research Areas

Cancer Biomarkers

Citations for Simple Plex Human Progranulin/PGRN Cartridge

View all citations for the Simple Plex platform.

Please visit our Instrument Citation Database to search our collection of scientific articles that feature our instruments.

Customer Reviews for Simple Plex Human Progranulin/PGRN Cartridge

There are currently no reviews for this product. Be the first to review Simple Plex Human Progranulin/PGRN Cartridge and earn rewards!

Have you used Simple Plex Human Progranulin/PGRN Cartridge?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 for a review with an image

$10/€7/£6/$10CAN/¥1110 for a review without an image

Submit a review
Amazon Gift Card