Simple Plex Human Syndecan-4 Assay Cartridge
R&D Systems | Catalog # SPCKB-PS-009775
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Simple Plex cartridges are custom built to fit your needs. Choose from over 350 assays spanning human, mouse, rat, and non-human primate. Just select your sample type, cartridge size (16, 32, or 72 samples), and up to 8 analytes per cartridge. Customize your cartridge on Bio-Techne.com
Key Product Details
Assay Length
Sample Type & Volume Required Per Well*
Sensitivity
Assay Range
Simple Plex Human Syndecan-4 Assay Cartridge Summary
*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.
Compatible Instruments
Ella Automated Immunoassay System
Product Specifications
Assay Type
Format
Species
Specificity
Cross-reactivity
Interference
精度
Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay.
Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents.
Cell Culture Supernates, Cerebrospinal Fluid, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 16 | 16 | 47 | 46 |
| Mean (pg/mL) | 55.4 | 2887 | 59.0 | 2958 |
| Standard Deviation | 4.55 | 150 | 5.70 | 259 |
| CV% | 8.2 | 5.2 | 9.7 | 8.8 |
Recovery for Simple Plex Human Syndecan-4 Assay Cartridge
Recovery at three different spiked concentrations within the range of the assay was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=2) | 102 | 99-105 |
| Cerebrospinal Fluid (n=3) | 109 | 104-115 |
Linearity
Samples containing and/or spiked with high concentrations of human Syndecan-4 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.
Scientific Data Images for Simple Plex Human Syndecan-4 Assay Cartridge
Human Syndecan-4 Ella Assay Standard Curve
Preparation and Storage
Shipping
Stability & Storage
Background: Syndecan-4
Syndecans are type 1 transmembrane proteoglycans that constitute the major physiological form of heparan sulfate (HS) on the cell surface. The four vertebrate syndecans, Syndecan-1 through -4, have similar short cytoplasmic domains and extracellular portions that diverge, except for HS attachment sites. Structurally diverse side chains add considerably to the size of the core proteins and serve as binding sites for growth factors, cytokines, and extracellular matrix proteins. Syndecans are present as homodimers or multimers, and are often expressed in developmental and cell type-specific patterns. They have many biological roles including regulating cell growth, differentiation, and adhesion.
Alternate Names
Gene Symbol
Additional Syndecan-4 Products
Product Documents for Simple Plex Human Syndecan-4 Assay Cartridge
Product Specific Notices for Simple Plex Human Syndecan-4 Assay Cartridge
For research use only
Citations for Simple Plex Human Syndecan-4 Assay Cartridge
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