Simple Plex Mouse CXCL12/SDF-1 alpha Cartridge

R&D Systems | Catalog # SPCKB-MP-001192

Simple Plex Mouse CXCL12/SDF-1 alpha assay kit for use on Ella instrument. Contains cartridge, sample diluent SD09, and wash buffer.
R&D Systems

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Key Product Details

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Serum (25 µL)

Sensitivity

3.32 pg/mL

Assay Range

26-15860 pg/mL (Serum)

Simple Plex Mouse CXCL12/SDF-1 alpha Cartridge Summary

Simple Plex assay for the detection of mouse SDF-1 alpha in serum.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

Compatible Instruments

Ella Automated Immunoassay System

Ella Automated Immunoassay System

Efficiently analyze biomarkers with Ella's advanced technology, providing accurate, precise results in under 90 minutes. Quantify up to 8 targets per assay through a streamlined automated workflow. Choose from >350 highly validated Simple Plex assays or Simple Plex discovery assays.
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Product Specifications

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Species

Mouse

Specificity

Natural and recombinant mouse SDF-1 alpha.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

精度

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents.

Serum

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 2
n 16 16 21 22
Mean (pg/mL) 96.1 4737 91.9 4984
Standard Deviation 9.57 389 7.36 350
CV% 10.0 8.2 8.0 7.0

Recovery for Simple Plex Mouse CXCL12/SDF-1 alpha Cartridge

Recovery was not necessary as mouse SDF-1 alpha shows natural linearity data from a neat sample to a 1:16 dilution.

Linearity

Samples containing and/or spiked with high concentrations mouse SDF-1 alpha were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Mouse CXCL12/SDF-1 alpha Simple Plex Assay Linearity

Scientific Data Images for Simple Plex Mouse CXCL12/SDF-1 alpha Cartridge

Mouse CXCL12/SDF-1 alpha Simple Plex Assay Standard Curve

Mouse CXCL12/SDF-1 alpha Ella Assay Standard Curve

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: CXCL12/SDF-1 alpha

CXCL12 is a heparin-binding chemokine that regulates lymphopoiesis, enhances the survival of myeloid progenitor cells, regulates the patterning and cell number of neural progenitors, and promotes angiogenesis. CXCL12/SDF-1 alpha is one of several SDF-1 isoforms, all of which can undergo proteolytic processing of the first two N-terminal amino acids by DPPIV/CD26. SDF-1 isoforms signal through CXCR4 and CXCR7 and can also bind Syndecan-4. Their binding to CXCR4 may block the cellular entry of HIV.

 

Alternate Names

SDF1 alpha, SDF1a

Entrez Gene IDs

6387 (Human); 20315 (Mouse); 493806 (Feline)

Gene Symbol

CXCL12

Additional CXCL12/SDF-1 alpha Products

Product Documents for Simple Plex Mouse CXCL12/SDF-1 alpha Cartridge

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Certificate of Analysis

To download a Certificate of Analysis, please enter your Simple Plex catalog number and lot number in the search boxes below.

Product Specific Notices for Simple Plex Mouse CXCL12/SDF-1 alpha Cartridge

For research use only

Citations for Simple Plex Mouse CXCL12/SDF-1 alpha Cartridge

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