Simple Plex Mouse CXCL15/Lungkine Cartridge

Catalog #: SPCKB-MP-006576 Datasheet / COA / SDS

Simple Plex Mouse CXCL15/Lungkine assay kit for use on Ella instrument. Contains cartridge, sample diluent SD09, and wash buffer

Catalog #	Availability	Size / Price	Qty
SPCKB-MP-006576
SPCKA-MP-006576
SPCKC-MP-006576

3 Images

Product Details

Reviews

Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Simple Plex Mouse CXCL15/Lungkine Cartridge Summary

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Bronchoalveolar Lavage Fluid (5 uL), Cell Culture Supernates (10 uL), Serum (25 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL)

Sensitivity

1.37 pg/mL

Assay Range

10.9 - 16,640 pg/mL (Bronchoalveolar Lavage Fluid, Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)

Specificity

This assay recognizes natural and recombinant Lungkine

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

Product Summary

Simple Plex assay for the detection of mouse CXCL15/Lungkine in cell culture supernatant (CCS), serum, plasma (EDTA/Heparin), and bronchoalveolar lavage fluid (BALF).

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents.

Bronchoalveolar Lavage Fluid, Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	51	59
Mean (pg/mL)	111	5785	114	5818
Standard Deviation	7.69	286	12.1	516
CV%	6.9	4.9	10.6	8.9

Recovery

Recovery was not necessary as mouse Lungkine shows natural linearity data from a neat sample to a 1:16 dilution.

Linearity

Samples containing and/or spiked with high concentrations of CXCL15/Lungkine were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.