Simple Plex Mouse/Rat beta-Synuclein Cartridge

Catalog #: SPCKB-MP-010280 Datasheet / COA / SDS

Simple Plex Mouse/Rat beta-Synuclein assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer

Catalog #	Availability	Size / Price	Qty
SPCKB-MP-010280
SPCKA-MP-010280
SPCKC-MP-010280

Control Products Available

897123: Simple Plex Control for Human/Mouse/Rat beta-Synuclein

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Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Simple Plex Mouse/Rat beta-Synuclein Cartridge Summary

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Cell Culture Supernates (10 uL), Serum (25 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL), Cerebrospinal Fluid (25 uL)

Sensitivity

1.83 pg/mL

Assay Range

8.0 - 4,856 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Cerebrospinal Fluid)

Specificity

Natural and recombinant beta -Synuclein.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

Product Summary

Simple Plex assay for the detection of mouse and rat beta -Synuclein in cell culture supernates (CCS), serum, plasma (EDTA/Heparin), and cerebrospinal fluid (CSF).

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Cerebrospinal Fluid

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	41	40
Mean (pg/mL)	44.6	2168	44.3	2056
Standard Deviation	1.4	72.4	4.1	122
CV%	3.2	3.3	9.2	6

Recovery

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
Mouse Cell Culture Media (n=2)	105	100-109
Mouse EDTA Plasma (n=4)	102	94-105
Mouse Heparin Plasma (n=4)	102	95-107
Mouse Serum (n=4)	103	95-108
Rat EDTA Plasma (n=4)	104	98-111
Rat Heparin Plasma (n=4)	101	97-101
Rat Serum (n=4)	105	98-116

Linearity

Samples containing and/or spiked with high concentrations of beta -Synuclein were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Scientific Data

N/A

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Mouse/Rat beta-Synuclein Ella Assay Standard Curve

Product Datasheets

Product Datasheet

COA

SDS

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: beta-Synuclein

The synuclein family includes alpha-Synuclein, beta-Synuclein, gamma-Synuclein, and synoretin. These small, highly conserved proteins are localized predominantly in the brain, concentrated in presynaptic nerve terminals. Alpha-Synuclein is normally a soluble protein. Abnormal alpha-synuclein forms filamentous inclusions (Lewy bodies and Lewy neurites) in several neurodegenerative diseases, including Parkinson's disease (PD), dementia with Lewy bodies, and Alzheimer's disease. Beta-Synuclein and gamma-Synuclein (synoretin) associate with alpha-synuclein and inhibit its aggregation.

Entrez Gene IDs:

6620 (Human); 104069 (Mouse); 113893 (Rat)

Alternate Names:

beta-Synuclein; SNCB; synuclein, beta; Synuclein-beta