Simple Plex Non-Human Primate Chitinase 3-like 1 Cartridge

R&D Systems | Catalog # SPCKB-NP-016629

Simple Plex Non-Human Primate Chitinase 3-like 1 assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer. Sample type indicated by PS (Plasma/Serum).
R&D Systems

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Key Product Details

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Cell Culture Supernates (10 µL), Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL), Cerebrospinal Fluid (10 µL)

Sensitivity

2.36 pg/mL

Assay Range

6.68-25500 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Cerebrospinal Fluid)

Simple Plex Non-Human Primate Chitinase 3-like 1 Cartridge Summary

Simple Plex assay is for the detection of non-human primate Chitinase 3-like 1/YKL-40 (CHI3L1) in cell culture supernatant (CCS), serum, plasma (EDTA/Heparin), and cerebrospinal fluid (CSF) in Sample Diluent SD13.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View all Chitinase 3-like 1/YKL-40 Simple Plex »

Compatible Instruments

Ella Automated Immunoassay System

Ella Automated Immunoassay System

Efficiently analyze biomarkers with Ella's advanced technology, providing accurate, precise results in under 90 minutes. Quantify up to 8 targets per assay through a streamlined automated workflow. Choose from >350 highly validated Simple Plex assays or Simple Plex discovery assays.
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Product Specifications

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge with 3 Glass Nano Reactors (GNR)

Species

Non-human Primate

Specificity

Natural and recombinant CHI3L1. 

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

精度

Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one CHI3L1 assay.

Inter-Assay Precision (Precision between assays) Replicates of each CHI3L1 control were tested in multiple assays performed by at least three technicians using two lots of reagents for non-human primate CHI3L1.

Cell Culture Supernates, Cerebrospinal Fluid, EDTA Plasma, Heparin Plasma, Serum

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 2
n 16 16 18 17
Mean (pg/mL) 217 11535 205 11411
Standard Deviation 8.91 328 16.0 675
CV% 4.1 2.8 7.8 5.9

Recovery for Simple Plex Non-Human Primate Chitinase 3-like 1 Cartridge

Recovery for the non-human primate CHI3L1 assays was evaluated at three different spiked concentrations of CHI3L1 protein.

Sample Type Average % Recovery Range %
Cell Culture Media (n=2) 81 76-86

Linearity

Samples containing and/or spiked with high concentrations of CHI3L1 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Simple Plex Non-Human Primate CHI3L1 Assay Linearity

Scientific Data Images for Simple Plex Non-Human Primate Chitinase 3-like 1 Cartridge

Simple Plex Non-Human Primate CHI3L1 Assay Standard Curve

Simple Plex Non-Human Primate CHI3L1 Assay Standard Curve

Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Non-Human Primate CHI3L1 Assay. Assay range is 6.68-25,500 pg/mL.

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Chitinase 3-like 1/YKL-40

Chitinase 3-like 1/YKL-40 (CHI3L1), also called BRP39 in mouse, is a 39 kDa glycoprotein member of the glycosyl hydrolase 18 family although it does not show chitotriosidase activity. CHI3L1 is expressed by articular chondrocytes, synovial cells, activated monocyte-derived macrophages, neutrophils, endothelial cells, vascular smooth muscle cells, and some cancer cells. CHI3L1 binds to chitin and heparins, and it enhances cell adhesion, proliferation and tumor angiogenesis. Circulating CHI3L1 is elevated during inflammation and connective tissue remodeling such as arthritis, chronic obstructive pulmonary disease, diabetes, cardiovascular disease, inflammatory bowel disease, and liver cirrhosis. It is frequently upregulated in glioblastoma, myxoid chondrosarcoma, melanoma and carcinomas of the breast, thyroid, colon, lung, kidney, and ovary.

Alternate Names

CHI3L1, Chitinase 3 like 1, HCgp39, YKL-40

Entrez Gene IDs

1116 (Human); 12654 (Mouse); 102138036 (Cynomolgus Monkey)

Gene Symbol

CHI3L1

Additional Chitinase 3-like 1/YKL-40 Products

Product Documents for Simple Plex Non-Human Primate Chitinase 3-like 1 Cartridge

Certificate of Analysis

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Product Specific Notices for Simple Plex Non-Human Primate Chitinase 3-like 1 Cartridge

For research use only

Citations for Simple Plex Non-Human Primate Chitinase 3-like 1 Cartridge

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