Simple Plex Sepsis Panel

R&D Systems | Catalog # ST01C-PS-010887

Simple Plex 32x4 panel containing IL-6 3rd gen, Procalcitonin, TREM-1, uPAR with sample diluent SD13. Part # starting with ST01 ships in 2-3 days. Sample type indicated with PS (Plasma/Serum).
R&D Systems
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Key Product Details

Features

The Simple Plex Human Multianalyte Assay is for the detection of human IL-6 3rd gen, Procalcitonin, TREM-1, uPAR in plasma/serum samples.

 
Sample Type & Volume Required Per Well Plasma/Serum (25 uL)
Sensitivity IL-6 3rd gen 0.11 pg/mL; Procalcitonin 0.79 pg/mL; TREM-1 0.73 pg/mL; uPAR 0.2 pg/mL
Assay Range IL-6 3rd gen 0.28 - 2,652 pg/mL; Procalcitonin 1.58 - 15,100 pg/mL; TREM-1 4.2 - 40,000 pg/mL; uPAR 1.68 - 16,000 pg/mL

Species

Human

Applications

Simple Plex Automated Immunoassay

Product Summary for Simple Plex Sepsis Panel

Product Specifications

Reactivity Human
Specificity Natural and recombinant human IL-6 3rd gen, Procalcitonin, TREM-1, and uPAR.
Cross-reactivity <0.5% cross-reactivity observed with available related molecules. <50% cross-species reactivity observed with species tested.
Interference No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay
Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagent

    Intra-Assay Precision Inter-Assay Precision
  Sample 1 2 1 2
IL-6 3rd gen n 16 16 40 40
Mean (pg/mL) 23.1 876/td> 23.6 1008
Standard Deviation 0.899 72.1 1.82 77.7
CV (%) 3.9 8.2 7.7 7.7
Procalcitonin n 16 16 18 17
Mean (pg/mL) 81.2 4828 83.3 4876
Standard Deviation 5.15 301 4.69 321
CV (%) 6.3 6.2 5.6 6.6
TREM-1 n 16 16 24 23
Mean (pg/mL) 216 11,034 220 11,170
Standard Deviation 17.9 1013 20.1 673
CV (%) 8.3 9.2 9.1 6.0
uPAR n 16 16 36 35
Mean (pg/mL) 120 6099 110 5748
Standard Deviation 6.8 261 8.51 558
CV (%) 5.7 4.3 7.7 9.7

Recovery

Recovery at three different spiked concentrations within the range of the assay was evaluated.

  Sample Type Average % Recovery Range %
IL-6 3rd gen EDTA Plasma (n=4) 92 86-102
Heparin Plasma (n=4) 87 85-90
Serum (n=4) 94 90-99
Procalcitonin EDTA Plasma (n=4) 105 97-111
Heparin Plasma (n=4) 103 98-111
Serum (n=4) 105 96-110
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Formulation, Preparation, and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Multiplex Cytokine Assay Kits

Cytokine Multiplex Assay Kits allow researchers to simultaneously detect the levels of multiple cytokines in a single sample. This is particularly useful for identifying the wide array of changes that can be associated with different pathological conditions. For multianalyte cytokine detection, we offer membrane-based Proteome Profiler Cytokine Antibody Arrays, bead-based Luminex® Assays, and Simple Plex automated ELISAs. For our antibody arrays, we offer human, mouse, or rat XL Cytokine Antibody Arrays that detect 105, 111, or 79 analytes respectively, including interleukins, interferons, chemokines, and members of the tumor necrosis factor superfamily. Our standard Luminex Assays for human, mouse, rat, porcine, and non-human primate (NHP) may also be used to detect many of these same analytes. With a menu of more than 490 analytes to select from, multiplexing with our Luminex assays provides the ability to simultaneously assess up to 50 analytes using magnetic microparticles. Experience consistent biomarker detection and ultra-sensitive results in less than 90 minutes with Ella, our automated benchtop ELISA. Simple Plex offers an extensive selection of over 250 fully validated assays essential to complete your cytokine research. Whether you want a stocked panel, ready to ship in just 2 days, a custom panel with up to 8 assays specific to your research, or a single assay cartridge for up to 72 samples ran simultaneously, Simple Plex has the solution to your research needs.

Additional Multiplex Cytokine Assay Kits Products

Product Documents for Simple Plex Sepsis Panel

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Certificate of Analysis

To download a Certificate of Analysis, please enter your Simple Plex catalog number and lot number in the search boxes below.

Product Specific Notices for Simple Plex Sepsis Panel

For research use only

Citations for Simple Plex Sepsis Panel

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Please visit our Instrument Citation Database on Bio-Techne.com to search our collection of scientific articles that feature our instruments.

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