Human, Mouse SMN ELISA Kit (Colorimetric)

Novus Biologicals | Catalog # NBP2-62175

Novus Biologicals
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Key Product Details

Sample Type & Volume Required Per Well

Cell Lysates (100 uL)

Sensitivity

50pg/ml

Assay Range

50-3200pg/ml
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Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Species

Human, Mouse

Specificity

No cross reactivity.

精度

Intra-Assay Precision (Precision within an assay) Low Concentration %CV - 3.2% Medium Concentration %CV - 1.0% High - 0.8%

Inter-Assay Precision (Precision between assays) Low Concentration %CV - 11.4% Medium Concentration %CV - 8.9% High - 7.1%

Recovery for Human, Mouse SMN ELISA Kit (Colorimetric)

Recovery

Human PBMC Lysate Low - 99%
Medium - 100%
High - 100%

Mouse Brain Extract Low - 104%
Medium - 86%
High - 84%

Mouse Muscle Extract Low - 125%
Medium - 88%
High - 79%

Mouse Spinal Cord Extract Low - 44%<

Scientific Data Images for Human, Mouse SMN ELISA Kit (Colorimetric)

ELISA: Human, Mouse SMN ELISA Kit (Colorimetric) [NBP2-62175]

ELISA: Human, Mouse SMN ELISA Kit (Colorimetric) [NBP2-62175]

ELISA: Human, Mouse SMN ELISA Kit (Colorimetric) [NBP2-62175] - A typical standard curve for SMN ELISA kit. These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ELISA: Human, Mouse SMN ELISA Kit (Colorimetric) [NBP2-62175]

ELISA: Human, Mouse SMN ELISA Kit (Colorimetric) [NBP2-62175]

ELISA: Human, Mouse SMN ELISA Kit (Colorimetric) [NBP2-62175] - A parallelism experiment was carried out to determine if the recombinant human SMN standard accurately determines SMN concentrations in biological matrices. To assess parallelism, values for human PBMC lysate and mouse tissue extract was obtained from a s

Kit Contents for Human, Mouse SMN ELISA Kit (Colorimetric)

  • Antibody
  • Assay Buffer 13
  • Conjugate
  • Extraction Reagent 4
  • Microtiter Plate
  • Standard
  • Stop Solution 2
  • TMB Substrate
  • Wash Buffer Concentrate

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: SMN

SMN (Gemin 1) is the central component of a large oligomeric complex (SMN complex), including Gemins 2-7, that is necessary and sufficient for the in vivo assembly of Sm proteins onto the small nuclear (sn)RNAs that mediate pre-mRNA splicing. The SMN complex is found in the cytoplasm and nucleus. Within the nucleus, this protein localizes to subnuclear bodies called gems which are found near coiled bodies containing high concentrations of small ribonucleoproteins (snRNPs). Spinal muscular atrophy, a motor neuron disorder, results from reduced levels or a mutation in the Gemin 1 protein.

Alternate Names

Gemin-1, Kugelberg-Welander disease), SMNC, survival motor neuron protein, survival of motor neuron 1, telomeric

Gene Symbol

SMN1

Additional SMN Products

Product Documents for Human, Mouse SMN ELISA Kit (Colorimetric)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Human, Mouse SMN ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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FAQs for Human, Mouse SMN ELISA Kit (Colorimetric)

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  • Q: Could you recommond the Human specific SMN antibody? It will only recognize human SMN, without cross reaction with mouse smn.

    A: We do not have an SMN antibody that has been validated NOT to work in mouse samples. When an antibody is found to not react with a certain species, we mark the data sheet with a (-) next to the species' name. Unfortunately, the SMN antibodies that do not list mouse have not been tested in mouse, and may react.

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