SP1 Antibody (4B11) - Azide and BSA Free

Novus Biologicals | Catalog # H00006667-M05

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot, ELISA, Sandwich ELISA

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 4B11

Format

Azide and BSA Free
View all SP1 Primary Antibodies »

Product Specifications

Immunogen

SP1 (NP_612482, 522 a.a. ~ 618 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. QLSSMPGLQTINLSALGTSGIQVHPIQGLPLAIANAPGDHGAQLGLHGAGGDGIHDDTAGGEEGENSPDAQPQAGRRTRREACTCPYCKDSEGRGSG*

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Specificity

SP1 (4B11)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for SP1 Antibody (4B11) - Azide and BSA Free

Western Blot: SP1 Antibody (4B11) [H00006667-M05]

Western Blot: SP1 Antibody (4B11) [H00006667-M05]

Western Blot: SP1 Antibody (4B11) [H00006667-M05] - Analysis of SP1 expression in NIH/3T3 (Cat # L018V1).
Western Blot: SP1 Antibody (4B11) [H00006667-M05]

Western Blot: SP1 Antibody (4B11) [H00006667-M05]

Western Blot: SP1 Antibody (4B11) [H00006667-M05] - Analysis of SP1 expression in HeLa (Cat # L013V1).
Western Blot: SP1 Antibody (4B11) [H00006667-M05]

Western Blot: SP1 Antibody (4B11) [H00006667-M05]

Western Blot: SP1 Antibody (4B11) [H00006667-M05] - Analysis of SP1 expression in Raw 264.7 (Cat # L024V1).
Western Blot: SP1 Antibody (4B11) [H00006667-M05]

Western Blot: SP1 Antibody (4B11) [H00006667-M05]

Western Blot: SP1 Antibody (4B11) [H00006667-M05] - Analysis of SP1 expression in transfected 293T cell line by SP1 monoclonal antibody (M05), clone 4B11. Lane 1: SP1 transfected lysatE (80.693 KDa). Lane 2: Non-transfected lysate.
Sandwich ELISA: SP1 Antibody (4B11) [H00006667-M05] - Detection limit for recombinant GST tagged SP1 is approximately 10ng/ml as a capture antibody.

Applications for SP1 Antibody (4B11) - Azide and BSA Free

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Sandwich ELISA

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactivity against cell lysate, transfected lysate and recombinant protein for WB. It has been used for ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: SP1

Transcription factor that can activate or repress transcription in response to physiological and pathological stimuli. Binds with high affinity to GC-rich motifs and regulates the expression of a large number of genes involved in a variety of processes such as cell growth, apoptosis, differentiation and immune responses. Highly regulated by post-translational modifications (phosphorylations, sumoylation, proteolytic cleavage, glycosylation and acetylation). Binds also the PDGFR-alpha G-box promoter. May have a role in modulating the cellular response to DNA damage. Implicated in chromatin remodeling

Alternate Names

Sp1 transcription factor, specificity protein 1, transcription factor Sp1, TSFP1

Entrez Gene IDs

6667 (Human)

Gene Symbol

SP1

OMIM

189906 (Human)

UniProt

NP_612482 (Human)

Additional SP1 Products

Product Documents for SP1 Antibody (4B11) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for SP1 Antibody (4B11) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for SP1 Antibody (4B11) - Azide and BSA Free

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