Mouse SPINK5 ELISA Kit (Colorimetric)

Novus Biologicals | Catalog # NBP3-53557

Novus Biologicals

Key Product Details

Sample Type & Volume Required Per Well

Tissue homogenates, cell lysates and other biological fluids (100 uL)

Sensitivity

28 pg/mL (example only; lot dependent)

Assay Range

78.125 - 5000 pg/mL (example only; lot dependent)
View all LEKTI/SPINK5 ELISA Kits »

Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Species

Mouse

Description

Assay Length: 4.5 hours

精度

Intra-Assay Precision (Precision within an assay) %CV 10 (example only; lot dependent)

Inter-Assay Precision (Precision between assays) %CV 12 (example only; lot dependent)

Kit Contents for Mouse SPINK5 ELISA Kit (Colorimetric)

  • Pre-coated 96T strip plate, Plate sealer for 96 wells, Standard, Diluent Buffer, Detection Reagent A, Detection Reagent B, TMB Substrate, Stop Solution, Wash Buffer (30 x concentrate), Instruction manual

Preparation and Storage

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: LEKTI/SPINK5

LEKTI, encoded by the SPINK5 gene, is a serine proteinase inhibitor with activity toward Kallikreins, Plasmin, Subtilisin A, Cathepsin G, Elastase, and Trypsin. It is primarily expressed in differentiated keratinocytes and is found in multiple forms due to alternative splicing and proteolytic cleavage. In the skin, LEKTI regulates the ability of KLK5, KLK7, and KLK14 to degrade the desmosomal proteins Desmoglein-1 and Desmoplakin-1 during desquamation. Mutations in SPINK5 and loss of its expression are associated with the development of various skin disorders such as Netherton syndrome, atopic eczema, and atopic dermatitis.

Long Name

Serine Peptidase Inhibitor, Kazal Type 5

Alternate Names

LETKI, NETS, SPINK5, VAKTI

Gene Symbol

SPINK5

Additional LEKTI/SPINK5 Products

Product Documents for Mouse SPINK5 ELISA Kit (Colorimetric)

Certificate of Analysis

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Product Specific Notices for Mouse SPINK5 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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